Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle
Objective: There are a few studies related to nitric oxide (NO) and leptin interaction in the regulation of physiological events in skeletal muscle. Therefore, the aim of the present study is to investigate the interaction of leptin and NO in response to blood flow and nitric oxide synthase (NOS) di...
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Istanbul University Press
2022-06-01
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| Series: | European Journal of Biology |
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| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/B3A769BF61D3439583BC2EB1CE835716 |
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| author | Savaş Üstünova Ayşegül Kapucu Cihan Demirci Tansel |
| author_facet | Savaş Üstünova Ayşegül Kapucu Cihan Demirci Tansel |
| author_sort | Savaş Üstünova |
| collection | DOAJ |
| description | Objective: There are a few studies related to nitric oxide (NO) and leptin interaction in the regulation of physiological events in skeletal muscle. Therefore, the aim of the present study is to investigate the interaction of leptin and NO in response to blood flow and nitric oxide synthase (NOS) distribution on rat skeletal muscle. Materials and Methods: Twenty-four adult-male Wistar albino rats were divided into 4 groups: control (C), Leptin (LP) (50 μg/kg), L-NG-nitroarginine methyl ester (LN) (a non-specific nitric oxide synthase inhibitor, 10 mg/kg,) and L-NAME+Leptin (LN+LP) administrated groups. Drugs were administered via the right jugular vein. Hemodynamic parameters: mean arterial pressure, heart rate and blood flow were recorded during the experiment and at the end blood samples for biochemical analyses of leptin and nitrite/nitrate levels and gastrocnemius muscle tissues from the right hindlimb for NOS distribution were taken. Results: Leptin infusion after L-NAME administration significantly decreased heart rate and blood flow (p<0.05, p<0.001). In addition, there was no change in the mean arterial pressure in the leptin group and leptin-administered L-NAME group. Leptin levels were the highest in the LP+LN group (p<0.01). Decreased nitrate/nitrate levels with L-NAME administration (p<0.01 vs C) reached control values by leptin infusion. Both endothelial NOS (eNOS) and neuronal NOS (nNOS) distributions were observed in the skeletal muscle cells of the leptin group. Conclusion: Although NO synthesis is inhibited by L-NAME, it is concluded that leptin partially enhances NO production and leptin uses NO as a mediator in its physiological effects. |
| format | Article |
| id | doaj-art-ddc6f7954b0147508f027595c032db7b |
| institution | OA Journals |
| issn | 2618-6144 |
| language | English |
| publishDate | 2022-06-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-ddc6f7954b0147508f027595c032db7b2025-08-20T02:10:39ZengIstanbul University PressEuropean Journal of Biology2618-61442022-06-01811182510.26650/EurJBiol.2022.1082814123456Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal MuscleSavaş Üstünova0https://orcid.org/0000-0003-1870-229XAyşegül Kapucu1https://orcid.org/0000-0002-0946-1407Cihan Demirci Tansel2https://orcid.org/0000-0001-7926-3089Bezmiâlem Vakıf Üniversitesi, Istanbul, Turkiyeİstanbul Üniversitesi, İstanbul, Türkiyeİstanbul Üniversitesi, İstanbul, TürkiyeObjective: There are a few studies related to nitric oxide (NO) and leptin interaction in the regulation of physiological events in skeletal muscle. Therefore, the aim of the present study is to investigate the interaction of leptin and NO in response to blood flow and nitric oxide synthase (NOS) distribution on rat skeletal muscle. Materials and Methods: Twenty-four adult-male Wistar albino rats were divided into 4 groups: control (C), Leptin (LP) (50 μg/kg), L-NG-nitroarginine methyl ester (LN) (a non-specific nitric oxide synthase inhibitor, 10 mg/kg,) and L-NAME+Leptin (LN+LP) administrated groups. Drugs were administered via the right jugular vein. Hemodynamic parameters: mean arterial pressure, heart rate and blood flow were recorded during the experiment and at the end blood samples for biochemical analyses of leptin and nitrite/nitrate levels and gastrocnemius muscle tissues from the right hindlimb for NOS distribution were taken. Results: Leptin infusion after L-NAME administration significantly decreased heart rate and blood flow (p<0.05, p<0.001). In addition, there was no change in the mean arterial pressure in the leptin group and leptin-administered L-NAME group. Leptin levels were the highest in the LP+LN group (p<0.01). Decreased nitrate/nitrate levels with L-NAME administration (p<0.01 vs C) reached control values by leptin infusion. Both endothelial NOS (eNOS) and neuronal NOS (nNOS) distributions were observed in the skeletal muscle cells of the leptin group. Conclusion: Although NO synthesis is inhibited by L-NAME, it is concluded that leptin partially enhances NO production and leptin uses NO as a mediator in its physiological effects.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/B3A769BF61D3439583BC2EB1CE835716blood flowleptinnitric oxiderat; skeletal muscle |
| spellingShingle | Savaş Üstünova Ayşegül Kapucu Cihan Demirci Tansel Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle European Journal of Biology blood flow leptin nitric oxide rat; skeletal muscle |
| title | Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle |
| title_full | Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle |
| title_fullStr | Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle |
| title_full_unstemmed | Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle |
| title_short | Leptin Enhances Nitric Oxide Production and Decreases Blood Flow in Rat Skeletal Muscle |
| title_sort | leptin enhances nitric oxide production and decreases blood flow in rat skeletal muscle |
| topic | blood flow leptin nitric oxide rat; skeletal muscle |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/B3A769BF61D3439583BC2EB1CE835716 |
| work_keys_str_mv | AT savasustunova leptinenhancesnitricoxideproductionanddecreasesbloodflowinratskeletalmuscle AT aysegulkapucu leptinenhancesnitricoxideproductionanddecreasesbloodflowinratskeletalmuscle AT cihandemircitansel leptinenhancesnitricoxideproductionanddecreasesbloodflowinratskeletalmuscle |