Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells
ABSTRACT Background The development of novel therapies for sarcoma is urgently required because most sarcomas are refractory to adjuvant therapy and the treatment options are limited. Although antitumor effects of blue light (BL) have been reported for several malignant tumors, its impact on various...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-03-01
|
| Series: | Cancer Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/cam4.70770 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850150550757179392 |
|---|---|
| author | Shinji Kawaguchi Toshihiko Nishisho Shunichi Toki Makoto Takeuchi Shunsuke Tamaki Koichi Sairyo |
| author_facet | Shinji Kawaguchi Toshihiko Nishisho Shunichi Toki Makoto Takeuchi Shunsuke Tamaki Koichi Sairyo |
| author_sort | Shinji Kawaguchi |
| collection | DOAJ |
| description | ABSTRACT Background The development of novel therapies for sarcoma is urgently required because most sarcomas are refractory to adjuvant therapy and the treatment options are limited. Although antitumor effects of blue light (BL) have been reported for several malignant tumors, its impact on various sarcomas remains unknown. In this study, we examined the antitumor effects of BL on several kinds of bone and soft tissue sarcomas. Methods We used human osteosarcoma, undifferentiated pleomorphic sarcoma, liposarcoma, and myxofibrosarcoma cell lines, as well as a human dermal fibroblast cell line as normal cells. We continuously irradiated these cells with BL in an incubator. Results BL inhibited cell proliferation in sarcoma cells, but hardly affected normal cells. BL increased intracellular reactive oxygen species (ROS) levels in sarcoma cells, but not in normal cells. Interestingly, oxidative stress occurred after BL irradiation in both sarcoma and normal cells. In addition, apoptosis, autophagy, and mitochondrial dysfunction, which were induced by ROS, occurred in sarcoma cells. In undifferentiated pleomorphic sarcoma cells, BL caused cell death through activation of the endogenous apoptotic pathway, and autophagy counteracted the apoptosis. Conclusion Our results indicate that BL might specifically kill sarcoma cells without injuring normal cells and may become a new treatment option for sarcoma. |
| format | Article |
| id | doaj-art-ddc4199ba374411bbfb7c362218ccf3a |
| institution | OA Journals |
| issn | 2045-7634 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Cancer Medicine |
| spelling | doaj-art-ddc4199ba374411bbfb7c362218ccf3a2025-08-20T02:26:31ZengWileyCancer Medicine2045-76342025-03-01146n/an/a10.1002/cam4.70770Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal CellsShinji Kawaguchi0Toshihiko Nishisho1Shunichi Toki2Makoto Takeuchi3Shunsuke Tamaki4Koichi Sairyo5Department of Orthopedics, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima JapanDepartment of Orthopedics, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima JapanDepartment of Orthopedics, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima JapanDepartment of Orthopedics, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima JapanDepartment of Orthopedics, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima JapanDepartment of Orthopedics, Institute of Biomedical Sciences Tokushima University Graduate School Tokushima JapanABSTRACT Background The development of novel therapies for sarcoma is urgently required because most sarcomas are refractory to adjuvant therapy and the treatment options are limited. Although antitumor effects of blue light (BL) have been reported for several malignant tumors, its impact on various sarcomas remains unknown. In this study, we examined the antitumor effects of BL on several kinds of bone and soft tissue sarcomas. Methods We used human osteosarcoma, undifferentiated pleomorphic sarcoma, liposarcoma, and myxofibrosarcoma cell lines, as well as a human dermal fibroblast cell line as normal cells. We continuously irradiated these cells with BL in an incubator. Results BL inhibited cell proliferation in sarcoma cells, but hardly affected normal cells. BL increased intracellular reactive oxygen species (ROS) levels in sarcoma cells, but not in normal cells. Interestingly, oxidative stress occurred after BL irradiation in both sarcoma and normal cells. In addition, apoptosis, autophagy, and mitochondrial dysfunction, which were induced by ROS, occurred in sarcoma cells. In undifferentiated pleomorphic sarcoma cells, BL caused cell death through activation of the endogenous apoptotic pathway, and autophagy counteracted the apoptosis. Conclusion Our results indicate that BL might specifically kill sarcoma cells without injuring normal cells and may become a new treatment option for sarcoma.https://doi.org/10.1002/cam4.70770blue lightendogenous apoptosisnormal human dermal cellosteosarcomareactive oxygen speciessoft tissue sarcoma |
| spellingShingle | Shinji Kawaguchi Toshihiko Nishisho Shunichi Toki Makoto Takeuchi Shunsuke Tamaki Koichi Sairyo Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells Cancer Medicine blue light endogenous apoptosis normal human dermal cell osteosarcoma reactive oxygen species soft tissue sarcoma |
| title | Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells |
| title_full | Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells |
| title_fullStr | Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells |
| title_full_unstemmed | Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells |
| title_short | Blue Light Emitting Diode Suppresses Sarcoma Cell Proliferation via the Endogenous Apoptotic Pathway Without Damaging Normal Cells |
| title_sort | blue light emitting diode suppresses sarcoma cell proliferation via the endogenous apoptotic pathway without damaging normal cells |
| topic | blue light endogenous apoptosis normal human dermal cell osteosarcoma reactive oxygen species soft tissue sarcoma |
| url | https://doi.org/10.1002/cam4.70770 |
| work_keys_str_mv | AT shinjikawaguchi bluelightemittingdiodesuppressessarcomacellproliferationviatheendogenousapoptoticpathwaywithoutdamagingnormalcells AT toshihikonishisho bluelightemittingdiodesuppressessarcomacellproliferationviatheendogenousapoptoticpathwaywithoutdamagingnormalcells AT shunichitoki bluelightemittingdiodesuppressessarcomacellproliferationviatheendogenousapoptoticpathwaywithoutdamagingnormalcells AT makototakeuchi bluelightemittingdiodesuppressessarcomacellproliferationviatheendogenousapoptoticpathwaywithoutdamagingnormalcells AT shunsuketamaki bluelightemittingdiodesuppressessarcomacellproliferationviatheendogenousapoptoticpathwaywithoutdamagingnormalcells AT koichisairyo bluelightemittingdiodesuppressessarcomacellproliferationviatheendogenousapoptoticpathwaywithoutdamagingnormalcells |