Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers
Bee venom (BV) and its components, secretory phospholipase A2 (sPLA2) and Apis cerana secapin-1 (AcSecapin-1), have potential effects on wound healing. This study aims to evaluate impact of BV, sPLA2, and AcSecapin-1 on full-thickness wound healing in male Wistar Albino rats over a 7-day period. Var...
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Sciendo
2024-09-01
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| Series: | Acta Veterinaria |
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| Online Access: | https://doi.org/10.2478/acve-2024-0025 |
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| author | Denk Bariş Yaprakci Volkan Dayi Belma Sevimli Alper Stevanović Jevrosima Glavinić Uroš Stanimirović Zoran |
| author_facet | Denk Bariş Yaprakci Volkan Dayi Belma Sevimli Alper Stevanović Jevrosima Glavinić Uroš Stanimirović Zoran |
| author_sort | Denk Bariş |
| collection | DOAJ |
| description | Bee venom (BV) and its components, secretory phospholipase A2 (sPLA2) and Apis cerana secapin-1 (AcSecapin-1), have potential effects on wound healing. This study aims to evaluate impact of BV, sPLA2, and AcSecapin-1 on full-thickness wound healing in male Wistar Albino rats over a 7-day period. Various morphometric (body weight, wound contraction), biochemical (hydroxyproline, oleic acid, IL-8, TGF-β1, redox parameters), and histopathological markers (reepithelialization, inflammatory cells, angiogenesis, fibroblast activation, and collagen density) were assessed. Treatment with sPLA2 and AcSecapin-1 increased oleic acid levels. IL-8 levels increased with sPLA2 treatment, and TGF-β1 levels increased with AcSecapin-1 treatment. BV and its components led to a decrease in FRAP levels. Additionally, BV treatment resulted in reduced angiogenesis, and both BV and sPLA2 treatments reduced inflammatory cells. All groups exhibited wound contraction without delay or regression. sPLA2 and AcSecapin-1 induced alterations in the wound healing milieu, without systemic changes. The treatment groups, except for the AcSecapin-1 group, showed an anti-inflammatory effect, identified by reduced inflammatory cell accumulation. Only the BV treatment suppressed angiogenesis. In conclusion, BV, sPLA2, and AcSecapin-1 demonstrate distinct effects on wound healing, with BV showing notable anti-inflammatory and anti-angiogenic properties, while sPLA2 and AcSecapin-1 influenced cytokine and oleic acid levels. |
| format | Article |
| id | doaj-art-ddb5a27687804f58a88a2b69bc65a762 |
| institution | OA Journals |
| issn | 1820-7448 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Veterinaria |
| spelling | doaj-art-ddb5a27687804f58a88a2b69bc65a7622025-08-20T01:47:50ZengSciendoActa Veterinaria1820-74482024-09-0174334736610.2478/acve-2024-0025Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological MarkersDenk Bariş0Yaprakci Volkan1Dayi Belma2Sevimli Alper3Stevanović Jevrosima4Glavinić Uroš5Stanimirović Zoran61Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Biochemistry, Afyonkarahisar, Turkiye2Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Surgery, Afyonkarahisar, Turkiye3Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pathology, Afyonkarahisar, Turkiye3Afyon Kocatepe University, Faculty of Veterinary Medicine, Department of Pathology, Afyonkarahisar, Turkiye4University of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Serbia4University of Belgrade, Faculty of Veterinary Medicine, Department of Biology, Serbia4University of Belgrade, Faculty of Veterinary Medicine, Department of Biology, SerbiaBee venom (BV) and its components, secretory phospholipase A2 (sPLA2) and Apis cerana secapin-1 (AcSecapin-1), have potential effects on wound healing. This study aims to evaluate impact of BV, sPLA2, and AcSecapin-1 on full-thickness wound healing in male Wistar Albino rats over a 7-day period. Various morphometric (body weight, wound contraction), biochemical (hydroxyproline, oleic acid, IL-8, TGF-β1, redox parameters), and histopathological markers (reepithelialization, inflammatory cells, angiogenesis, fibroblast activation, and collagen density) were assessed. Treatment with sPLA2 and AcSecapin-1 increased oleic acid levels. IL-8 levels increased with sPLA2 treatment, and TGF-β1 levels increased with AcSecapin-1 treatment. BV and its components led to a decrease in FRAP levels. Additionally, BV treatment resulted in reduced angiogenesis, and both BV and sPLA2 treatments reduced inflammatory cells. All groups exhibited wound contraction without delay or regression. sPLA2 and AcSecapin-1 induced alterations in the wound healing milieu, without systemic changes. The treatment groups, except for the AcSecapin-1 group, showed an anti-inflammatory effect, identified by reduced inflammatory cell accumulation. Only the BV treatment suppressed angiogenesis. In conclusion, BV, sPLA2, and AcSecapin-1 demonstrate distinct effects on wound healing, with BV showing notable anti-inflammatory and anti-angiogenic properties, while sPLA2 and AcSecapin-1 influenced cytokine and oleic acid levels.https://doi.org/10.2478/acve-2024-0025angiogenesishoneybeeoleic acidphospholipase a2secapinwound healing |
| spellingShingle | Denk Bariş Yaprakci Volkan Dayi Belma Sevimli Alper Stevanović Jevrosima Glavinić Uroš Stanimirović Zoran Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers Acta Veterinaria angiogenesis honeybee oleic acid phospholipase a2 secapin wound healing |
| title | Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers |
| title_full | Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers |
| title_fullStr | Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers |
| title_full_unstemmed | Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers |
| title_short | Exploring the Therapeutic Potential of Bee Venom Components in Wound Healing: A Comprehensive Evaluation of Morphometric, Biochemical, and Histopathological Markers |
| title_sort | exploring the therapeutic potential of bee venom components in wound healing a comprehensive evaluation of morphometric biochemical and histopathological markers |
| topic | angiogenesis honeybee oleic acid phospholipase a2 secapin wound healing |
| url | https://doi.org/10.2478/acve-2024-0025 |
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