Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows
Mastitis is the leading cause of economic losses in dairy farming, significantly impairing animal welfare and the quality and quantity of milk production. MicroRNAs are increasingly gaining attention, in both human and veterinary medicine, as biomarkers for various diseases. This study evaluated the...
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MDPI AG
2025-02-01
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| Online Access: | https://www.mdpi.com/2218-273X/15/2/235 |
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| author | Eleonora Dall’Olio Fabio De Rensis Eugenio Martignani Silvia Miretti Ugo Ala Valeria Cavalli Claudio Cipolat-Gotet Melania Andrani Mario Baratta Roberta Saleri |
| author_facet | Eleonora Dall’Olio Fabio De Rensis Eugenio Martignani Silvia Miretti Ugo Ala Valeria Cavalli Claudio Cipolat-Gotet Melania Andrani Mario Baratta Roberta Saleri |
| author_sort | Eleonora Dall’Olio |
| collection | DOAJ |
| description | Mastitis is the leading cause of economic losses in dairy farming, significantly impairing animal welfare and the quality and quantity of milk production. MicroRNAs are increasingly gaining attention, in both human and veterinary medicine, as biomarkers for various diseases. This study evaluated the diagnostic potential of four circulating microRNAs (miR-26-5p, miR-142-5p, miR-146a, and miR-223-3p) by examining changes in their expression in milk samples from dairy cows at different immune-cell subpopulations correlated to different stage of mastitis with a validated method. Additionally, this study has analyzed the possible source of these circulating microRNAs by the measurement of their secretion from activated immune cells (lymphocytes, monocytes, and neutrophils). miR-223-3p has been significantly expressed in an acute stage of mastitis (<i>p</i> < 0.01) but not in the chronic or susceptible stages. Conversely, mir-26-5p has been significantly reduced in acute, chronic, and susceptible groups of animals. In immune-cell cultures, miR-26 has been shown to be down-regulated in lipopolysaccharide (LPS)-stimulated neutrophils, while miR-223 has been shown to be up-regulated in phytohemagglutinin (PHA)-stimulated lymphocytes. The differential expression of miR-223-3p and miR-26-5p, combined with differential and total somatic cell count, could serve as a useful tool for identifying the evolutionary stage of mastitis-related inflammatory pathology. |
| format | Article |
| id | doaj-art-ddb58697843d45a29f4e729d0862090b |
| institution | DOAJ |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj-art-ddb58697843d45a29f4e729d0862090b2025-08-20T02:44:59ZengMDPI AGBiomolecules2218-273X2025-02-0115223510.3390/biom15020235Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy CowsEleonora Dall’Olio0Fabio De Rensis1Eugenio Martignani2Silvia Miretti3Ugo Ala4Valeria Cavalli5Claudio Cipolat-Gotet6Melania Andrani7Mario Baratta8Roberta Saleri9Department of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Sciences, University of Torino, Largo Paolo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyDepartment of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Parco Area delle Scienze 11a, 43124 Parma, ItalyDepartment of Veterinary Science, University of Parma, Strada del Taglio 10, 43126 Parma, ItalyMastitis is the leading cause of economic losses in dairy farming, significantly impairing animal welfare and the quality and quantity of milk production. MicroRNAs are increasingly gaining attention, in both human and veterinary medicine, as biomarkers for various diseases. This study evaluated the diagnostic potential of four circulating microRNAs (miR-26-5p, miR-142-5p, miR-146a, and miR-223-3p) by examining changes in their expression in milk samples from dairy cows at different immune-cell subpopulations correlated to different stage of mastitis with a validated method. Additionally, this study has analyzed the possible source of these circulating microRNAs by the measurement of their secretion from activated immune cells (lymphocytes, monocytes, and neutrophils). miR-223-3p has been significantly expressed in an acute stage of mastitis (<i>p</i> < 0.01) but not in the chronic or susceptible stages. Conversely, mir-26-5p has been significantly reduced in acute, chronic, and susceptible groups of animals. In immune-cell cultures, miR-26 has been shown to be down-regulated in lipopolysaccharide (LPS)-stimulated neutrophils, while miR-223 has been shown to be up-regulated in phytohemagglutinin (PHA)-stimulated lymphocytes. The differential expression of miR-223-3p and miR-26-5p, combined with differential and total somatic cell count, could serve as a useful tool for identifying the evolutionary stage of mastitis-related inflammatory pathology.https://www.mdpi.com/2218-273X/15/2/235mastitisdairy cowsbiomarkersdifferential somatic cell countmiR-223miR-26 |
| spellingShingle | Eleonora Dall’Olio Fabio De Rensis Eugenio Martignani Silvia Miretti Ugo Ala Valeria Cavalli Claudio Cipolat-Gotet Melania Andrani Mario Baratta Roberta Saleri Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows Biomolecules mastitis dairy cows biomarkers differential somatic cell count miR-223 miR-26 |
| title | Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows |
| title_full | Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows |
| title_fullStr | Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows |
| title_full_unstemmed | Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows |
| title_short | Differential Expression of miR-223-3p and miR-26-5p According to Different Stages of Mastitis in Dairy Cows |
| title_sort | differential expression of mir 223 3p and mir 26 5p according to different stages of mastitis in dairy cows |
| topic | mastitis dairy cows biomarkers differential somatic cell count miR-223 miR-26 |
| url | https://www.mdpi.com/2218-273X/15/2/235 |
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