Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model
Objective(s): Acute myocardial infarction causes the heart to lose its proper function due to contractile dysfunction within the damaged ischemic cardiac tissue. Different studies have presented various cardiac patches based on different biomaterials to support the infarcted myocardium to recover ca...
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Mashhad University of Medical Sciences
2025-04-01
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| Series: | Nanomedicine Journal |
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| Online Access: | https://nmj.mums.ac.ir/article_25204_1987f08f748c2862b60a3d6689df976f.pdf |
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| author | Safieh Boroumand Parisa Ahmadi Faraz Sigaroodi Mohammad Mahdi Khani Fatemeh Ghanbari Shahram Rabbani Hossein Ahmadi Tafti Asieh Heirani-Tabasi Hossein Ghanbari |
| author_facet | Safieh Boroumand Parisa Ahmadi Faraz Sigaroodi Mohammad Mahdi Khani Fatemeh Ghanbari Shahram Rabbani Hossein Ahmadi Tafti Asieh Heirani-Tabasi Hossein Ghanbari |
| author_sort | Safieh Boroumand |
| collection | DOAJ |
| description | Objective(s): Acute myocardial infarction causes the heart to lose its proper function due to contractile dysfunction within the damaged ischemic cardiac tissue. Different studies have presented various cardiac patches based on different biomaterials to support the infarcted myocardium to recover cardiac function. Materials and Methods: In this study, we developed a nanofiber cardiac patch with antioxidant and antibacterial properties. Polycaprolactone (PCL) nanofibers were enriched with chitosan-coated selenium nanoparticles (Cs-SeNPs) using an electrospinning technique. Results: The PCL/Cs-SeNPs nanofibers, with an average diameter of 648.36±259.19 nm, displayed antioxidant properties in the DPPH assay. Additionally, viable cell count assessment demonstrated the antibacterial effects of PCL/Cs-SeNPs nanofibers against Staphylococcus aureus and Escherichia coli bacteria. MTT assay results revealed improved proliferation for the PCL/Cs-SeNPs nanofibers compared to the PCL nanofibers scaffold, with no significant cell toxicity. The SEM imaging and DAPI/Phalloidin staining supported the improved cell adhesion with well-expanded cytoskeleton of 3T3 cells on PCL/Cs-SeNPs nanofibers. In the rat model of myocardial infarction, improved cardiac function and reduced post-surgical adhesion were observed 28 days after surgery. Conclusion: The results of this study suggest that the PCL/Cs-SeNPs nanofibers cardiac patch can be considered a potent supportive strategy for myocardial rehabilitation after myocardial infarction. |
| format | Article |
| id | doaj-art-ddb21b9bdace4cf496cdc25a58c09408 |
| institution | DOAJ |
| issn | 2322-3049 2322-5904 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Nanomedicine Journal |
| spelling | doaj-art-ddb21b9bdace4cf496cdc25a58c094082025-08-20T02:46:07ZengMashhad University of Medical SciencesNanomedicine Journal2322-30492322-59042025-04-0112223825110.22038/nmj.2024.79242.195025204Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI modelSafieh Boroumand0Parisa Ahmadi1Faraz Sigaroodi2Mohammad Mahdi Khani3Fatemeh Ghanbari4Shahram Rabbani5Hossein Ahmadi Tafti6Asieh Heirani-Tabasi7Hossein Ghanbari8Research Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranSchool of Medicine, Tehran University of Medical Sciences, Tehran, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranDepartment of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Cardiovascular Diseases Research Institute, Tehran University of Medical Sciences, Tehran, IranObjective(s): Acute myocardial infarction causes the heart to lose its proper function due to contractile dysfunction within the damaged ischemic cardiac tissue. Different studies have presented various cardiac patches based on different biomaterials to support the infarcted myocardium to recover cardiac function. Materials and Methods: In this study, we developed a nanofiber cardiac patch with antioxidant and antibacterial properties. Polycaprolactone (PCL) nanofibers were enriched with chitosan-coated selenium nanoparticles (Cs-SeNPs) using an electrospinning technique. Results: The PCL/Cs-SeNPs nanofibers, with an average diameter of 648.36±259.19 nm, displayed antioxidant properties in the DPPH assay. Additionally, viable cell count assessment demonstrated the antibacterial effects of PCL/Cs-SeNPs nanofibers against Staphylococcus aureus and Escherichia coli bacteria. MTT assay results revealed improved proliferation for the PCL/Cs-SeNPs nanofibers compared to the PCL nanofibers scaffold, with no significant cell toxicity. The SEM imaging and DAPI/Phalloidin staining supported the improved cell adhesion with well-expanded cytoskeleton of 3T3 cells on PCL/Cs-SeNPs nanofibers. In the rat model of myocardial infarction, improved cardiac function and reduced post-surgical adhesion were observed 28 days after surgery. Conclusion: The results of this study suggest that the PCL/Cs-SeNPs nanofibers cardiac patch can be considered a potent supportive strategy for myocardial rehabilitation after myocardial infarction.https://nmj.mums.ac.ir/article_25204_1987f08f748c2862b60a3d6689df976f.pdfchitosanmyocardial infarctionnanofiberspolycaprolactoneselenium nanoparticles |
| spellingShingle | Safieh Boroumand Parisa Ahmadi Faraz Sigaroodi Mohammad Mahdi Khani Fatemeh Ghanbari Shahram Rabbani Hossein Ahmadi Tafti Asieh Heirani-Tabasi Hossein Ghanbari Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model Nanomedicine Journal chitosan myocardial infarction nanofibers polycaprolactone selenium nanoparticles |
| title | Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model |
| title_full | Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model |
| title_fullStr | Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model |
| title_full_unstemmed | Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model |
| title_short | Selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat MI model |
| title_sort | selenium nanoparticles loaded nanofibers ameliorate cardiac function after acute myocardial infarction in rat mi model |
| topic | chitosan myocardial infarction nanofibers polycaprolactone selenium nanoparticles |
| url | https://nmj.mums.ac.ir/article_25204_1987f08f748c2862b60a3d6689df976f.pdf |
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