Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal
Peanut is a key cash crop worldwide, yet the limited availability of functional genes and markers for breeding hinders further progress, largely due to the lack of an efficient and user-friendly transformation system. This study aimed to comprehensively evaluate the effectiveness of nodal agroinject...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/15/2/384 |
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| author | Malizukiswe Vincent Vacu Chunjiao Jiang Haojie Sun Guangdi Yuan Jing Yu Jun Zhang Chuantang Wang |
| author_facet | Malizukiswe Vincent Vacu Chunjiao Jiang Haojie Sun Guangdi Yuan Jing Yu Jun Zhang Chuantang Wang |
| author_sort | Malizukiswe Vincent Vacu |
| collection | DOAJ |
| description | Peanut is a key cash crop worldwide, yet the limited availability of functional genes and markers for breeding hinders further progress, largely due to the lack of an efficient and user-friendly transformation system. This study aimed to comprehensively evaluate the effectiveness of nodal agroinjection, a novel transformation technique we developed for peanut, by introducing the soybean cold-tolerance gene <i>SCTF-1</i>. Putative transgenic seeds and seedlings were screened using genomic DNA PCR, while transgene expression was analyzed via qRT-PCR and phenotypic assessments. Southern blotting confirmed the stable integration of <i>SCTF-1</i>. The transgenic seedlings displayed enhanced chilling tolerance, characterized by increased proline accumulation, reduced malondialdehyde (MDA), and elevated peroxidase (POD) activity. These findings demonstrate that nodal agroinjection is an efficient and reliable approach for generating transgenic peanut and analyzing gene function. This method offers a promising alternative to conventional tissue culture-based transformation strategies. |
| format | Article |
| id | doaj-art-dda9ae64d0fb4897be138012f5dbbaf7 |
| institution | DOAJ |
| issn | 2073-4395 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj-art-dda9ae64d0fb4897be138012f5dbbaf72025-08-20T02:44:36ZengMDPI AGAgronomy2073-43952025-01-0115238410.3390/agronomy15020384Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An AppraisalMalizukiswe Vincent Vacu0Chunjiao Jiang1Haojie Sun2Guangdi Yuan3Jing Yu4Jun Zhang5Chuantang Wang6Shandong Peanut Research Institute (SPRI), Qingdao 266100, ChinaShandong Peanut Research Institute (SPRI), Qingdao 266100, ChinaShandong Peanut Research Institute (SPRI), Qingdao 266100, ChinaShandong Peanut Research Institute (SPRI), Qingdao 266100, ChinaShandong Peanut Research Institute (SPRI), Qingdao 266100, ChinaCollege of Agronomy, Jilin Agricultural University, Changchun 130118, ChinaShandong Peanut Research Institute (SPRI), Qingdao 266100, ChinaPeanut is a key cash crop worldwide, yet the limited availability of functional genes and markers for breeding hinders further progress, largely due to the lack of an efficient and user-friendly transformation system. This study aimed to comprehensively evaluate the effectiveness of nodal agroinjection, a novel transformation technique we developed for peanut, by introducing the soybean cold-tolerance gene <i>SCTF-1</i>. Putative transgenic seeds and seedlings were screened using genomic DNA PCR, while transgene expression was analyzed via qRT-PCR and phenotypic assessments. Southern blotting confirmed the stable integration of <i>SCTF-1</i>. The transgenic seedlings displayed enhanced chilling tolerance, characterized by increased proline accumulation, reduced malondialdehyde (MDA), and elevated peroxidase (POD) activity. These findings demonstrate that nodal agroinjection is an efficient and reliable approach for generating transgenic peanut and analyzing gene function. This method offers a promising alternative to conventional tissue culture-based transformation strategies.https://www.mdpi.com/2073-4395/15/2/384<i>Agrobacterium tumefaciens</i>peanutgenetic transformationcold tolerancenodal injection |
| spellingShingle | Malizukiswe Vincent Vacu Chunjiao Jiang Haojie Sun Guangdi Yuan Jing Yu Jun Zhang Chuantang Wang Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal Agronomy <i>Agrobacterium tumefaciens</i> peanut genetic transformation cold tolerance nodal injection |
| title | Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal |
| title_full | Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal |
| title_fullStr | Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal |
| title_full_unstemmed | Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal |
| title_short | Nodal Injection of <i>Agrobacterium tumefaciens</i> for Gene Functional Analysis in Peanut: An Appraisal |
| title_sort | nodal injection of i agrobacterium tumefaciens i for gene functional analysis in peanut an appraisal |
| topic | <i>Agrobacterium tumefaciens</i> peanut genetic transformation cold tolerance nodal injection |
| url | https://www.mdpi.com/2073-4395/15/2/384 |
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