Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats
Background Hexavalent chromium [Cr(VI)] is a heavy metal, extensively used in the stainless steel industry. It causes various metabolic oxidative stress and is hepatotoxic. 1,25-Dihydroxy vitamin-D3 (1,25(OH)2D3) synthesized in the body is an antioxidant against metabolic oxidative stress. Objective...
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| Language: | English |
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Wolters Kluwer Medknow Publications
2024-10-01
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| Series: | Medical Journal of Dr. D.Y. Patil Vidyapeeth |
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| Online Access: | https://journals.lww.com/10.4103/mjdrdypu.mjdrdypu_156_24 |
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| author | Saeed M. Yendigeri Surekha U. Arakeri Kusal K. Das |
| author_facet | Saeed M. Yendigeri Surekha U. Arakeri Kusal K. Das |
| author_sort | Saeed M. Yendigeri |
| collection | DOAJ |
| description | Background
Hexavalent chromium [Cr(VI)] is a heavy metal, extensively used in the stainless steel industry. It causes various metabolic oxidative stress and is hepatotoxic. 1,25-Dihydroxy vitamin-D3 (1,25(OH)2D3) synthesized in the body is an antioxidant against metabolic oxidative stress.
Objectives
The present study aimed to assess the possible protective effects of 1,25(OH)2D3 supplementation on Cr(VI) induced hepatotoxicity in albino rats.
Methods
Twenty-four male adult Wister rats were divided into four groups (n = 6 in each group) as follows: Group-1 (control); group-2 (K2Cr2O7, 5.0 mg/kg body weight intraperitoneally for 10 dosages on every alternate day), group-3 (1,25(OH)2D3, 12.5 μg/kg/d, orally daily till 20 days) and group-4 (K2Cr2O7+1,25(OH)2D3). At the end of the 10th dosage after overnight fasting, i.e., (on day 21) gravimetry was obtained, blood samples were collected for analysis of hematology, liver function tests, serum oxidative stress markers (MDA and SOD), nitrosative stress markers like nitric oxide (NO), antioxidants (ascorbic acid, α-tocopherol, and vitamin D3) were estimated. Histopathology of the liver was done after sacrificing the animals.
Results
Cr(VI)-treated rats showed a significant alteration of hematological parameters, liver enzymes, and oxidative and nitrosative stress parameters as compared to their respective controls. Simultaneous supplementation of 1,25(OH)2D3 with Cr(VI) showed significant beneficial effects on rodent hematology, oxidative stress, and antioxidant defence system against hexavalent chromium-induced hepatotoxicities. Histopathology of the liver showed several hepatic damages in Cr(VI)-induced group. The recorded data showed that liver damages in the 1,25(OH)2D3 supplemented group were partially protected hepatic tissues.
Conclusion
This study showed 1,25(OH)2D3 as a hepatoprotective antioxidant against hexavalent chromium-induced hepatotoxicity. |
| format | Article |
| id | doaj-art-dd938b76ae7b4f2eb16add3cb4715a90 |
| institution | OA Journals |
| issn | 2589-8302 2589-8310 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Medical Journal of Dr. D.Y. Patil Vidyapeeth |
| spelling | doaj-art-dd938b76ae7b4f2eb16add3cb4715a902025-08-20T02:12:42ZengWolters Kluwer Medknow PublicationsMedical Journal of Dr. D.Y. Patil Vidyapeeth2589-83022589-83102024-10-0117Suppl 1S186S19210.4103/mjdrdypu.mjdrdypu_156_24Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino RatsSaeed M. YendigeriSurekha U. ArakeriKusal K. DasBackground Hexavalent chromium [Cr(VI)] is a heavy metal, extensively used in the stainless steel industry. It causes various metabolic oxidative stress and is hepatotoxic. 1,25-Dihydroxy vitamin-D3 (1,25(OH)2D3) synthesized in the body is an antioxidant against metabolic oxidative stress. Objectives The present study aimed to assess the possible protective effects of 1,25(OH)2D3 supplementation on Cr(VI) induced hepatotoxicity in albino rats. Methods Twenty-four male adult Wister rats were divided into four groups (n = 6 in each group) as follows: Group-1 (control); group-2 (K2Cr2O7, 5.0 mg/kg body weight intraperitoneally for 10 dosages on every alternate day), group-3 (1,25(OH)2D3, 12.5 μg/kg/d, orally daily till 20 days) and group-4 (K2Cr2O7+1,25(OH)2D3). At the end of the 10th dosage after overnight fasting, i.e., (on day 21) gravimetry was obtained, blood samples were collected for analysis of hematology, liver function tests, serum oxidative stress markers (MDA and SOD), nitrosative stress markers like nitric oxide (NO), antioxidants (ascorbic acid, α-tocopherol, and vitamin D3) were estimated. Histopathology of the liver was done after sacrificing the animals. Results Cr(VI)-treated rats showed a significant alteration of hematological parameters, liver enzymes, and oxidative and nitrosative stress parameters as compared to their respective controls. Simultaneous supplementation of 1,25(OH)2D3 with Cr(VI) showed significant beneficial effects on rodent hematology, oxidative stress, and antioxidant defence system against hexavalent chromium-induced hepatotoxicities. Histopathology of the liver showed several hepatic damages in Cr(VI)-induced group. The recorded data showed that liver damages in the 1,25(OH)2D3 supplemented group were partially protected hepatic tissues. Conclusion This study showed 1,25(OH)2D3 as a hepatoprotective antioxidant against hexavalent chromium-induced hepatotoxicity.https://journals.lww.com/10.4103/mjdrdypu.mjdrdypu_156_241,25(oh)2d3chromium (vi) [cr(vi)]hepatotoxicity and oxidative stress |
| spellingShingle | Saeed M. Yendigeri Surekha U. Arakeri Kusal K. Das Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats Medical Journal of Dr. D.Y. Patil Vidyapeeth 1,25(oh)2d3 chromium (vi) [cr(vi)] hepatotoxicity and oxidative stress |
| title | Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats |
| title_full | Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats |
| title_fullStr | Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats |
| title_full_unstemmed | Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats |
| title_short | Effect of 1,25-Dihydroxy Vitamin D3 (1,25(OH)2D3) Supplementation on Hexavalent Chromium [Cr(VI)]-Induced Hepatoxicities in Male Albino Rats |
| title_sort | effect of 1 25 dihydroxy vitamin d3 1 25 oh 2d3 supplementation on hexavalent chromium cr vi induced hepatoxicities in male albino rats |
| topic | 1,25(oh)2d3 chromium (vi) [cr(vi)] hepatotoxicity and oxidative stress |
| url | https://journals.lww.com/10.4103/mjdrdypu.mjdrdypu_156_24 |
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