Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species
The rapid spread of <i>tet</i>(X) genes capable of inactivating tigecycline represents a critical challenge to global public health. This study aims to explore the distribution, genetic diversity, and transferability of <i>tet</i>(X) genes in <i>Myroides</i>, a ge...
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2025-05-01
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| author | Chong Chen Taotao Wu Jing Liu Yilin Lv |
| author_facet | Chong Chen Taotao Wu Jing Liu Yilin Lv |
| author_sort | Chong Chen |
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| description | The rapid spread of <i>tet</i>(X) genes capable of inactivating tigecycline represents a critical challenge to global public health. This study aims to explore the distribution, genetic diversity, and transferability of <i>tet</i>(X) genes in <i>Myroides</i>, a genus of Gram-negative bacteria increasingly implicated in multidrug-resistant (MDR) bacterial infections. From 2021 to 2024, 646 samples of chicken, sheep, soil, and water were randomly collected, yielding nine chicken-derived <i>tet</i>(X)-positive <i>Myroides</i> sp. strains in Shandong, China. All of them were MDR to tetracycline, ceftazidime, gentamicin, amikacin, colistin, ciprofloxacin, gatifloxacin, and trimethoprim-sulfamethoxazole, with elevated minimum inhibitory concentrations (MICs) for tigecycline, florfenicol, and macrolides, but exhibited susceptibility to meropenem (100%), ampicillin-sulbactam (66.7%), and cefotaxime (33.3%). A genomic analysis of the isolates and 86 public <i>tet</i>(X)-positive <i>Myroides</i> genomes revealed the widespread distribution of <i>tet</i>(X) and macrolide-inactivating <i>estT</i> genes across 12 <i>Myroides</i> species, including 7 novel species. Eight <i>tet</i>(X) and eight estT variants were identified, half of which were novel. The phylogenetic analysis highlighted interspecies transmission risks, with IS<i>CR2</i>-mediated transposons of <i>tet</i>(X6) and <i>estT-2</i> across <i>Myroides</i>, <i>Riemerella</i>, <i>Empedobacter</i>, <i>Providencia</i>, <i>Acinetobacter</i>, and <i>Proteus</i> species. These findings illuminate the genomic diversity driving antibiotic resistance in understudied bacterial taxa, with implications for global One Health strategies. |
| format | Article |
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| institution | OA Journals |
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| language | English |
| publishDate | 2025-05-01 |
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| series | Microorganisms |
| spelling | doaj-art-dd9136edabaa4f298bb98beb7819aa3a2025-08-20T02:20:59ZengMDPI AGMicroorganisms2076-26072025-05-01136118010.3390/microorganisms13061180Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> SpeciesChong Chen0Taotao Wu1Jing Liu2Yilin Lv3Joint International Research Laboratory of Agriculture and Agri-Product Safety, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, ChinaJoint International Research Laboratory of Agriculture and Agri-Product Safety, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, ChinaThe rapid spread of <i>tet</i>(X) genes capable of inactivating tigecycline represents a critical challenge to global public health. This study aims to explore the distribution, genetic diversity, and transferability of <i>tet</i>(X) genes in <i>Myroides</i>, a genus of Gram-negative bacteria increasingly implicated in multidrug-resistant (MDR) bacterial infections. From 2021 to 2024, 646 samples of chicken, sheep, soil, and water were randomly collected, yielding nine chicken-derived <i>tet</i>(X)-positive <i>Myroides</i> sp. strains in Shandong, China. All of them were MDR to tetracycline, ceftazidime, gentamicin, amikacin, colistin, ciprofloxacin, gatifloxacin, and trimethoprim-sulfamethoxazole, with elevated minimum inhibitory concentrations (MICs) for tigecycline, florfenicol, and macrolides, but exhibited susceptibility to meropenem (100%), ampicillin-sulbactam (66.7%), and cefotaxime (33.3%). A genomic analysis of the isolates and 86 public <i>tet</i>(X)-positive <i>Myroides</i> genomes revealed the widespread distribution of <i>tet</i>(X) and macrolide-inactivating <i>estT</i> genes across 12 <i>Myroides</i> species, including 7 novel species. Eight <i>tet</i>(X) and eight estT variants were identified, half of which were novel. The phylogenetic analysis highlighted interspecies transmission risks, with IS<i>CR2</i>-mediated transposons of <i>tet</i>(X6) and <i>estT-2</i> across <i>Myroides</i>, <i>Riemerella</i>, <i>Empedobacter</i>, <i>Providencia</i>, <i>Acinetobacter</i>, and <i>Proteus</i> species. These findings illuminate the genomic diversity driving antibiotic resistance in understudied bacterial taxa, with implications for global One Health strategies.https://www.mdpi.com/2076-2607/13/6/1180<i>Myroides</i> spp.tetracyclines<i>tet</i>(X)macrolides<i>estT</i>IS<i>CR2</i> |
| spellingShingle | Chong Chen Taotao Wu Jing Liu Yilin Lv Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species Microorganisms <i>Myroides</i> spp. tetracyclines <i>tet</i>(X) macrolides <i>estT</i> IS<i>CR2</i> |
| title | Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species |
| title_full | Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species |
| title_fullStr | Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species |
| title_full_unstemmed | Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species |
| title_short | Genomic Diversity of the <i>tet</i>(X)-Positive <i>Myroides</i> Species |
| title_sort | genomic diversity of the i tet i x positive i myroides i species |
| topic | <i>Myroides</i> spp. tetracyclines <i>tet</i>(X) macrolides <i>estT</i> IS<i>CR2</i> |
| url | https://www.mdpi.com/2076-2607/13/6/1180 |
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