Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1.
Lyme disease is caused by spirochaetes of the Borrelia burgdorferi sensu lato genospecies. Complete genome assemblies are available for fewer than ten strains of Borrelia burgdorferi sensu stricto, the primary cause of Lyme disease in North America. MM1 is a sensu stricto strain originally isolated...
Saved in:
| _version_ | 1850076692343685120 |
|---|---|
| author | Neda Jabbari Gustavo Glusman Lena M Joesch-Cohen Panga Jaipal Reddy Robert L Moritz Leroy Hood Christopher G Lausted |
| author_facet | Neda Jabbari Gustavo Glusman Lena M Joesch-Cohen Panga Jaipal Reddy Robert L Moritz Leroy Hood Christopher G Lausted |
| author_sort | Neda Jabbari |
| collection | DOAJ |
| description | Lyme disease is caused by spirochaetes of the Borrelia burgdorferi sensu lato genospecies. Complete genome assemblies are available for fewer than ten strains of Borrelia burgdorferi sensu stricto, the primary cause of Lyme disease in North America. MM1 is a sensu stricto strain originally isolated in the midwestern United States. Aside from a small number of genes, the complete genome sequence of this strain has not been reported. Here we present the complete genome sequence of MM1 in relation to other sensu stricto strains and in terms of its Multi Locus Sequence Typing. Our results indicate that MM1 is a new sequence type which contains a conserved main chromosome and 15 plasmids. Our results include the first contiguous 28.5 kb assembly of lp28-8, a linear plasmid carrying the vls antigenic variation system, from a Borrelia burgdorferi sensu stricto strain. |
| format | Article |
| id | doaj-art-dd9099edf16c4fc2a6fe8ccba0f03f26 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-dd9099edf16c4fc2a6fe8ccba0f03f262025-08-20T02:45:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019813510.1371/journal.pone.0198135Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1.Neda JabbariGustavo GlusmanLena M Joesch-CohenPanga Jaipal ReddyRobert L MoritzLeroy HoodChristopher G LaustedLyme disease is caused by spirochaetes of the Borrelia burgdorferi sensu lato genospecies. Complete genome assemblies are available for fewer than ten strains of Borrelia burgdorferi sensu stricto, the primary cause of Lyme disease in North America. MM1 is a sensu stricto strain originally isolated in the midwestern United States. Aside from a small number of genes, the complete genome sequence of this strain has not been reported. Here we present the complete genome sequence of MM1 in relation to other sensu stricto strains and in terms of its Multi Locus Sequence Typing. Our results indicate that MM1 is a new sequence type which contains a conserved main chromosome and 15 plasmids. Our results include the first contiguous 28.5 kb assembly of lp28-8, a linear plasmid carrying the vls antigenic variation system, from a Borrelia burgdorferi sensu stricto strain.https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198135/1/pone.0198135.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210221%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210221T172056Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=7a6c2006364a108d53ac7dcac9f355725c47adb7c6c91840f7d172a919d16a4c4dcffca4cd96f4ef1595a1828ecbddbde82232ede6386707144464b2784d03153a5cefc9eff82821f52ae42972d31f66a0e7532074a209727bef073fa273753216a4d1d1f80f58b3bf8e1a9bad7443fbd1903b671856a8bcadc70174abb1d145435dfa80b62f4bf9e12b0922eb80f7b365737352a35e14654a60e9c4425f7d18685038b5cb6a5180ae898e7a7a4091ca50bcaa255d9a8043f2fb32626c4976ccd07376b8a219eb2b4e07ce3f2bca815e09b5cb0bb2491dd335c417eae005590b09dac4b2d099246c78236e11a445734d7c9a208aab894c830fd392a92eb969ae |
| spellingShingle | Neda Jabbari Gustavo Glusman Lena M Joesch-Cohen Panga Jaipal Reddy Robert L Moritz Leroy Hood Christopher G Lausted Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1. PLoS ONE |
| title | Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1. |
| title_full | Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1. |
| title_fullStr | Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1. |
| title_full_unstemmed | Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1. |
| title_short | Whole genome sequence and comparative analysis of Borrelia burgdorferi MM1. |
| title_sort | whole genome sequence and comparative analysis of borrelia burgdorferi mm1 |
| url | https://storage.googleapis.com/plos-corpus-prod/10.1371/journal.pone.0198135/1/pone.0198135.pdf?X-Goog-Algorithm=GOOG4-RSA-SHA256&X-Goog-Credential=wombat-sa%40plos-prod.iam.gserviceaccount.com%2F20210221%2Fauto%2Fstorage%2Fgoog4_request&X-Goog-Date=20210221T172056Z&X-Goog-Expires=3600&X-Goog-SignedHeaders=host&X-Goog-Signature=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 |
| work_keys_str_mv | AT nedajabbari wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 AT gustavoglusman wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 AT lenamjoeschcohen wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 AT pangajaipalreddy wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 AT robertlmoritz wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 AT leroyhood wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 AT christopherglausted wholegenomesequenceandcomparativeanalysisofborreliaburgdorferimm1 |