How do amoebae swim and crawl?
The surface behaviour of swimming amoebae was followed in cells bearing a cAR1-paGFP (cyclic AMP receptor fused to a photoactivatable-GFP) construct. Sensitized amoebae were placed in a buoyant medium where they could swim toward a chemoattractant cAMP source. paGFP, activated at the cell's fro...
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0074382&type=printable |
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| author | Jonathan D Howe Nicholas P Barry Mark S Bretscher |
| author_facet | Jonathan D Howe Nicholas P Barry Mark S Bretscher |
| author_sort | Jonathan D Howe |
| collection | DOAJ |
| description | The surface behaviour of swimming amoebae was followed in cells bearing a cAR1-paGFP (cyclic AMP receptor fused to a photoactivatable-GFP) construct. Sensitized amoebae were placed in a buoyant medium where they could swim toward a chemoattractant cAMP source. paGFP, activated at the cell's front, remained fairly stationary in the cell's frame as the cell advanced; the label was not swept rearwards. Similar experiments with chemotaxing cells attached to a substratum gave the same result. Furthermore, if the region around a lateral projection near a crawling cell's front is marked, the projection and the labelled cAR1 behave differently. The label spreads by diffusion but otherwise remains stationary in the cell's frame; the lateral projection moves rearwards on the cell (remaining stationary with respect to the substrate), so that it ends up outside the labelled region. Furthermore, as cAR1-GFP cells move, they occasionally do so in a remarkably straight line; this suggests they do not need to snake to move on a substratum. Previously, we suggested that the surface membrane of a moving amoeba flows from front to rear as part of a polarised membrane trafficking cycle. This could explain how swimming amoebae are able to exert a force against the medium. Our present results indicate that, in amoebae, the suggested surface flow does not exist: this implies that they swim by shape changes. |
| format | Article |
| id | doaj-art-dd8a75eb254e48299bdcc3b7288a2605 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-dd8a75eb254e48299bdcc3b7288a26052025-08-20T02:35:44ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7438210.1371/journal.pone.0074382How do amoebae swim and crawl?Jonathan D HoweNicholas P BarryMark S BretscherThe surface behaviour of swimming amoebae was followed in cells bearing a cAR1-paGFP (cyclic AMP receptor fused to a photoactivatable-GFP) construct. Sensitized amoebae were placed in a buoyant medium where they could swim toward a chemoattractant cAMP source. paGFP, activated at the cell's front, remained fairly stationary in the cell's frame as the cell advanced; the label was not swept rearwards. Similar experiments with chemotaxing cells attached to a substratum gave the same result. Furthermore, if the region around a lateral projection near a crawling cell's front is marked, the projection and the labelled cAR1 behave differently. The label spreads by diffusion but otherwise remains stationary in the cell's frame; the lateral projection moves rearwards on the cell (remaining stationary with respect to the substrate), so that it ends up outside the labelled region. Furthermore, as cAR1-GFP cells move, they occasionally do so in a remarkably straight line; this suggests they do not need to snake to move on a substratum. Previously, we suggested that the surface membrane of a moving amoeba flows from front to rear as part of a polarised membrane trafficking cycle. This could explain how swimming amoebae are able to exert a force against the medium. Our present results indicate that, in amoebae, the suggested surface flow does not exist: this implies that they swim by shape changes.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0074382&type=printable |
| spellingShingle | Jonathan D Howe Nicholas P Barry Mark S Bretscher How do amoebae swim and crawl? PLoS ONE |
| title | How do amoebae swim and crawl? |
| title_full | How do amoebae swim and crawl? |
| title_fullStr | How do amoebae swim and crawl? |
| title_full_unstemmed | How do amoebae swim and crawl? |
| title_short | How do amoebae swim and crawl? |
| title_sort | how do amoebae swim and crawl |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0074382&type=printable |
| work_keys_str_mv | AT jonathandhowe howdoamoebaeswimandcrawl AT nicholaspbarry howdoamoebaeswimandcrawl AT marksbretscher howdoamoebaeswimandcrawl |