The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper
Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium r...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Tropical Medicine |
| Online Access: | http://dx.doi.org/10.1155/2022/5715436 |
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| author | Zhi-yong Tao Pei-yi Zhang Lu Zhang Chun-cao Li Rui Hu Han-wu Zhu Bei Zhou Kai Wu Ling-xu Li Da-wei Yao Yu-jie Cao Dao-jin Wang Chen-chen Zheng Run-qi Fang Xiu-min Hua Yi-xuan Ni Xiao-xia Jin Hui Xia Qiang Fang |
| author_facet | Zhi-yong Tao Pei-yi Zhang Lu Zhang Chun-cao Li Rui Hu Han-wu Zhu Bei Zhou Kai Wu Ling-xu Li Da-wei Yao Yu-jie Cao Dao-jin Wang Chen-chen Zheng Run-qi Fang Xiu-min Hua Yi-xuan Ni Xiao-xia Jin Hui Xia Qiang Fang |
| author_sort | Zhi-yong Tao |
| collection | DOAJ |
| description | Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas. |
| format | Article |
| id | doaj-art-dd76c25e5dbe4d28ac4dc31f70e26d25 |
| institution | OA Journals |
| issn | 1687-9694 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Tropical Medicine |
| spelling | doaj-art-dd76c25e5dbe4d28ac4dc31f70e26d252025-08-20T02:21:30ZengWileyJournal of Tropical Medicine1687-96942022-01-01202210.1155/2022/5715436The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter PaperZhi-yong Tao0Pei-yi Zhang1Lu Zhang2Chun-cao Li3Rui Hu4Han-wu Zhu5Bei Zhou6Kai Wu7Ling-xu Li8Da-wei Yao9Yu-jie Cao10Dao-jin Wang11Chen-chen Zheng12Run-qi Fang13Xiu-min Hua14Yi-xuan Ni15Xiao-xia Jin16Hui Xia17Qiang Fang18Department of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyChenzhou Center for Disease Control and PreventionCollege of Veterinary MedicineWuhan Center for Disease Control and PreventionCollege of Veterinary MedicineCollege of Veterinary MedicineDepartment of Microbiology and ParasitologyAnhui Key Laboratory of Infection and ImmunologyAnhui Key Laboratory of Infection and ImmunologyAnhui Key Laboratory of Infection and ImmunologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDepartment of Microbiology and ParasitologyDried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plasmodium. vivax (P. vivax) in archived DBS samples and Babesia gibsoni (B. gibsoni) in fresh pet DBS samples. Our results provided a useful reference for the detection of blood pathogens with DBS samples and direct PCR, especially for screening the cost-efficacy combination of filter paper and PCR kit in resource-limited areas.http://dx.doi.org/10.1155/2022/5715436 |
| spellingShingle | Zhi-yong Tao Pei-yi Zhang Lu Zhang Chun-cao Li Rui Hu Han-wu Zhu Bei Zhou Kai Wu Ling-xu Li Da-wei Yao Yu-jie Cao Dao-jin Wang Chen-chen Zheng Run-qi Fang Xiu-min Hua Yi-xuan Ni Xiao-xia Jin Hui Xia Qiang Fang The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper Journal of Tropical Medicine |
| title | The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper |
| title_full | The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper |
| title_fullStr | The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper |
| title_full_unstemmed | The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper |
| title_short | The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper |
| title_sort | comparison of pcr kits for the detection of erythrocytic parasites on filter paper |
| url | http://dx.doi.org/10.1155/2022/5715436 |
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