Diffusion models for super-resolution microscopy: a tutorial

Diffusion models have emerged as a prominent technique in generative modeling with neural networks, making their mark in tasks like text-to-image translation and super-resolution. In this tutorial, we provide a comprehensive guide to build denoising diffusion probabilistic models from scratch, with...

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Bibliographic Details
Main Authors: Harshith Bachimanchi, Giovanni Volpe
Format: Article
Language:English
Published: IOP Publishing 2025-01-01
Series:JPhys Photonics
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Online Access:https://doi.org/10.1088/2515-7647/ada101
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Summary:Diffusion models have emerged as a prominent technique in generative modeling with neural networks, making their mark in tasks like text-to-image translation and super-resolution. In this tutorial, we provide a comprehensive guide to build denoising diffusion probabilistic models from scratch, with a specific focus on transforming low-resolution microscopy images into their corresponding high-resolution versions in the context of super-resolution microscopy. We provide the necessary theoretical background, the essential mathematical derivations, and a detailed Python code implementation using PyTorch. We discuss the metrics to quantitatively evaluate the model, illustrate the model performance at different noise levels of the input low-resolution images, and briefly discuss how to adapt the tutorial for other applications. The code provided in this tutorial is also available as a Python notebook in the supplementary information.
ISSN:2515-7647