Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates
Introduction: Tuberculosis (TB) caused by the bacterium Mycobacterium tuberculosis remains a critical global public health concern due to the high morbidity and mortality rates. Mutation in atpE and Rv0678 genes contributes to drug resistance in M. tuberculosis. This study investigates the antib...
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| Format: | Article |
| Language: | English |
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Pasteur Institute of Iran
2023-12-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
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| author | Nahid Madadi-Goli1, 2, 3 Kamal Ahmadi1, 2 , 3 Sharareh Khanipour1, 2 Shahin Pourazar Dizaji1, 2 Mahshid Nasehi4, 5 Seyed Davar Siadat1, 2 Farzam Vaziri1, 2 Abolfazl Fateh1, 2* |
| author_facet | Nahid Madadi-Goli1, 2, 3 Kamal Ahmadi1, 2 , 3 Sharareh Khanipour1, 2 Shahin Pourazar Dizaji1, 2 Mahshid Nasehi4, 5 Seyed Davar Siadat1, 2 Farzam Vaziri1, 2 Abolfazl Fateh1, 2* |
| author_sort | Nahid Madadi-Goli1, 2, 3 |
| collection | DOAJ |
| description | Introduction: Tuberculosis (TB) caused by the bacterium Mycobacterium
tuberculosis remains a critical global public health concern due to the high
morbidity and mortality rates. Mutation in atpE and Rv0678 genes
contributes to drug resistance in M. tuberculosis. This study investigates the
antibiotic resistance patterns and mutations in atpE and Rv0678 genes in 22
M. tuberculosis clinical isolates. Methods: Drug susceptibility testing (DST)
for rifampin, isoniazid, streptomycin, capreomycin, ofloxacin, kanamycin,
and ethambutol was conducted using the proportional method. This was
followed by determining the minimum inhibitory concentration (MIC) for
bedaquiline (BDQ) via the microplate Alamar blue assay (MABA). Genomic
regions encompassing atpE and Rv0678 genes were amplified and
sequenced for mutation analysis. Data analysis was performed using SPSS
software to interpret mutation patterns concerning drug susceptibility
profiles. Results: Of 22 isolates, 5 (27.8%) were extensively drug-resistant
tuberculosis (XDR-TB), and 13 (72.2%) were multi-drug resistant
tuberculosis (MDR-TB). Resistance rates to kanamycin, ofloxacin,
capreomycin, and streptomycin were 40.6%, 46.3%, 85%, and 74.6%,
respectively. Additionally, phenotypic resistance to bedaquiline was
observed in 12 (54.5%) isolates. Sequencing revealed no resistanceconferring
mutations in the atpE or Rv0678 genes among the isolates.
Conclusion: Our findings showed substantial resistance to first- and secondline
drugs in M. tuberculosis clinical isolates. This highlights the necessity
for ongoing, comprehensive studies to elucidate the evolving drug resistance
patterns and understand the underlying mechanisms in clinical isolates. |
| format | Article |
| id | doaj-art-dd67ef63c0524925a8504dddae26fbf3 |
| institution | OA Journals |
| issn | 2345-5349 2345-5330 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Pasteur Institute of Iran |
| record_format | Article |
| series | Journal of Medical Microbiology and Infectious Diseases |
| spelling | doaj-art-dd67ef63c0524925a8504dddae26fbf32025-08-20T02:15:08ZengPasteur Institute of IranJournal of Medical Microbiology and Infectious Diseases2345-53492345-53302023-12-0111417918410.61186/JoMMID.11.4.179Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical IsolatesNahid Madadi-Goli1, 2, 30https://orcid.org/0000-0002-4531-3081Kamal Ahmadi1, 2 , 31https://orcid.org/0000-0002-9830-6651Sharareh Khanipour1, 22https://orcid.org/0009-0000-2188-136XShahin Pourazar Dizaji1, 23https://orcid.org/0009-0003-3960-6819Mahshid Nasehi4, 54https://orcid.org/0000-0002-2913-4294Seyed Davar Siadat1, 25https://orcid.org/0000-0002-6892-5603Farzam Vaziri1, 26https://orcid.org/0000-0001-7788-2548Abolfazl Fateh1, 2*7https://orcid.org/0000-0002-2043-85231Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran; 3Student Research Committee, Pasteur Institute of Iran, Tehran, Iran1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran; 3Student Research Committee, Pasteur Institute of Iran, Tehran, Iran1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran4Department of Epidemiology and Biostatistics, School of Public Health, Iran University of Medical Sciences, Tehran, Iran; 5Center for Communicable Diseases Control, Ministry of Health and Medical Education, Tehran, Iran1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran1Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; 2Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, IranIntroduction: Tuberculosis (TB) caused by the bacterium Mycobacterium tuberculosis remains a critical global public health concern due to the high morbidity and mortality rates. Mutation in atpE and Rv0678 genes contributes to drug resistance in M. tuberculosis. This study investigates the antibiotic resistance patterns and mutations in atpE and Rv0678 genes in 22 M. tuberculosis clinical isolates. Methods: Drug susceptibility testing (DST) for rifampin, isoniazid, streptomycin, capreomycin, ofloxacin, kanamycin, and ethambutol was conducted using the proportional method. This was followed by determining the minimum inhibitory concentration (MIC) for bedaquiline (BDQ) via the microplate Alamar blue assay (MABA). Genomic regions encompassing atpE and Rv0678 genes were amplified and sequenced for mutation analysis. Data analysis was performed using SPSS software to interpret mutation patterns concerning drug susceptibility profiles. Results: Of 22 isolates, 5 (27.8%) were extensively drug-resistant tuberculosis (XDR-TB), and 13 (72.2%) were multi-drug resistant tuberculosis (MDR-TB). Resistance rates to kanamycin, ofloxacin, capreomycin, and streptomycin were 40.6%, 46.3%, 85%, and 74.6%, respectively. Additionally, phenotypic resistance to bedaquiline was observed in 12 (54.5%) isolates. Sequencing revealed no resistanceconferring mutations in the atpE or Rv0678 genes among the isolates. Conclusion: Our findings showed substantial resistance to first- and secondline drugs in M. tuberculosis clinical isolates. This highlights the necessity for ongoing, comprehensive studies to elucidate the evolving drug resistance patterns and understand the underlying mechanisms in clinical isolates.mycobacterium tuberculosisdstatperv0678bedaquilineextensively drug-resistant tuberculosis (xdr-tb)multi-drug resistant tuberculosis (mdr-tb) |
| spellingShingle | Nahid Madadi-Goli1, 2, 3 Kamal Ahmadi1, 2 , 3 Sharareh Khanipour1, 2 Shahin Pourazar Dizaji1, 2 Mahshid Nasehi4, 5 Seyed Davar Siadat1, 2 Farzam Vaziri1, 2 Abolfazl Fateh1, 2* Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates Journal of Medical Microbiology and Infectious Diseases mycobacterium tuberculosis dst atpe rv0678 bedaquiline extensively drug-resistant tuberculosis (xdr-tb) multi-drug resistant tuberculosis (mdr-tb) |
| title | Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates |
| title_full | Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates |
| title_fullStr | Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates |
| title_full_unstemmed | Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates |
| title_short | Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates |
| title_sort | investigating the mutations in atpe and rv0678 genes in mycobacterium tuberculosis clinical isolates |
| topic | mycobacterium tuberculosis dst atpe rv0678 bedaquiline extensively drug-resistant tuberculosis (xdr-tb) multi-drug resistant tuberculosis (mdr-tb) |
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