A 96‐Well Ultrafiltration Approach for the High‐Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium

A 96‐Well Ultrafiltration Approach for the High‐Throughput Proteome Analysis of Extracellular Vesicles Isolated From Conditioned Medium

ABSTRACT Extracellular vesicles (EVs), nanoscale vesicles that are secreted by cells, are critical mediators of intercellular communication and play a crucial role in diverse pathologies such as cancer development. Therefore, EVs are regarded as having high potential in the clinic, both for diagnost...

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Bibliographic Details
Main Authors: Jarne Pauwels, Tessa Van de Steene, Freya De Muyer, Danaë De Pauw, Femke Baeke, Sven Eyckerman, Kris Gevaert
Format: Article
Language:English
Published: Wiley 2025-07-01
Series:Journal of Extracellular Vesicles
Subjects:
300 kDa MWCO 96‐well ultrafiltration
conditioned medium
proteomics
Tween‐20
Online Access:https://doi.org/10.1002/jev2.70103
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