Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway

Endometriosis is a benign disease with similar characteristics to tumors. Recent studies have found that the erythropoietin-producing hepatoma receptor A2 (EphA2) has the dual effect of promoting tumor and inhibiting tumor. The objective of this study was to explore the specific regulatory mechanism...

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Main Authors: Chaoyi Yang, Shujun Wang, Mengru Li, Xiangli Pang, Aili Tan
Format: Article
Language:English
Published: PAGEPress Publications 2025-06-01
Series:European Journal of Histochemistry
Subjects:
Online Access:https://www.ejh.it/ejh/article/view/4168
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author Chaoyi Yang
Shujun Wang
Mengru Li
Xiangli Pang
Aili Tan
author_facet Chaoyi Yang
Shujun Wang
Mengru Li
Xiangli Pang
Aili Tan
author_sort Chaoyi Yang
collection DOAJ
description Endometriosis is a benign disease with similar characteristics to tumors. Recent studies have found that the erythropoietin-producing hepatoma receptor A2 (EphA2) has the dual effect of promoting tumor and inhibiting tumor. The objective of this study was to explore the specific regulatory mechanism of EphA2 in endometriosis. The expression level of Eph protein family in endometriosis was analyzed by bioinformatics method. At the clinical level, qPCR, Western blot and immunohistochemistry were used to verify the correlation between increased EphA2 levels and endometriosis. The effects of blocking EphA2 on cell migration, invasion, proliferation and apoptosis of primary eutopic endometriotic stromal cells were explored in vitro. Our study indicated that EphA2 expression was elevated in endometriosis patients, and blocking EphA2 in vitro inhibited cell proliferation, migration and invasion through AMPK signaling pathway. Targeting EphA2 can inhibit the progression of endometriosis through the AMPK signaling pathway.
format Article
id doaj-art-dd613600042447e280cff2c94ab6ff95
institution DOAJ
issn 1121-760X
2038-8306
language English
publishDate 2025-06-01
publisher PAGEPress Publications
record_format Article
series European Journal of Histochemistry
spelling doaj-art-dd613600042447e280cff2c94ab6ff952025-08-20T03:11:39ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062025-06-0169310.4081/ejh.2025.4168Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathwayChaoyi Yang0Shujun Wang1Mengru Li2Xiangli Pang3Aili Tan4Department of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, HubeiDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, HubeiDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, HubeiDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, HubeiDepartment of Obstetrics and Gynecology, Renmin Hospital of Wuhan University, Wuhan, HubeiEndometriosis is a benign disease with similar characteristics to tumors. Recent studies have found that the erythropoietin-producing hepatoma receptor A2 (EphA2) has the dual effect of promoting tumor and inhibiting tumor. The objective of this study was to explore the specific regulatory mechanism of EphA2 in endometriosis. The expression level of Eph protein family in endometriosis was analyzed by bioinformatics method. At the clinical level, qPCR, Western blot and immunohistochemistry were used to verify the correlation between increased EphA2 levels and endometriosis. The effects of blocking EphA2 on cell migration, invasion, proliferation and apoptosis of primary eutopic endometriotic stromal cells were explored in vitro. Our study indicated that EphA2 expression was elevated in endometriosis patients, and blocking EphA2 in vitro inhibited cell proliferation, migration and invasion through AMPK signaling pathway. Targeting EphA2 can inhibit the progression of endometriosis through the AMPK signaling pathway. https://www.ejh.it/ejh/article/view/4168EndometriosisEphA2AMPK signaling pathwayALW-II-41-27
spellingShingle Chaoyi Yang
Shujun Wang
Mengru Li
Xiangli Pang
Aili Tan
Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway
European Journal of Histochemistry
Endometriosis
EphA2
AMPK signaling pathway
ALW-II-41-27
title Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway
title_full Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway
title_fullStr Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway
title_full_unstemmed Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway
title_short Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway
title_sort targeting epha2 suppresses the proliferation migration and invasion of endometriosis via the ampk signaling pathway
topic Endometriosis
EphA2
AMPK signaling pathway
ALW-II-41-27
url https://www.ejh.it/ejh/article/view/4168
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AT mengruli targetingepha2suppressestheproliferationmigrationandinvasionofendometriosisviatheampksignalingpathway
AT xianglipang targetingepha2suppressestheproliferationmigrationandinvasionofendometriosisviatheampksignalingpathway
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