Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood
ABSTRACT Extracellular vesicles (EVs) are nanosized, membrane‐bound particles released by virtually all cell types, serving as messengers within tissues and across organs via the bloodstream. EVs encapsulate diverse molecular cargo that reflects the phenotypic state of their originating cells, makin...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2025-06-01
|
| Series: | Journal of Extracellular Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/jex2.70059 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849430694804062208 |
|---|---|
| author | Lauren Newman Andrew Rowland |
| author_facet | Lauren Newman Andrew Rowland |
| author_sort | Lauren Newman |
| collection | DOAJ |
| description | ABSTRACT Extracellular vesicles (EVs) are nanosized, membrane‐bound particles released by virtually all cell types, serving as messengers within tissues and across organs via the bloodstream. EVs encapsulate diverse molecular cargo that reflects the phenotypic state of their originating cells, making them promising candidates for liquid biopsy applications. However, the heterogeneity of circulating EVs, comprising particles from various cell types and non‐vesicular entities like lipoproteins, poses significant challenges for isolating tissue‐specific EV populations. This review examines current methodologies for detecting and isolating tissue‐specific EVs from blood, focusing on immunoaffinity capture (IAC) strategies that leverage surface marker expression for specificity. Key considerations, including the selection and validation of markers, are discussed alongside advances in EV subtyping and isolation protocols. Challenges such as marker cross‐reactivity, EV biogenesis and transport dynamics are highlighted to underscore the complexity of achieving clinical utility. By providing an overview of validated tissue‐specific markers and isolation techniques, this review aims to facilitate the development of EV‐based biomarkers with enhanced specificity and sensitivity, enabling minimally invasive monitoring of organ function and disease. |
| format | Article |
| id | doaj-art-dd54dcb578db4400be94ac13ef58efec |
| institution | Kabale University |
| issn | 2768-2811 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Extracellular Biology |
| spelling | doaj-art-dd54dcb578db4400be94ac13ef58efec2025-08-20T03:27:53ZengWileyJournal of Extracellular Biology2768-28112025-06-0146n/an/a10.1002/jex2.70059Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the BloodLauren Newman0Andrew Rowland1College of Medicine and Public Health Flinders University Bedford Park South Australia AustraliaCollege of Medicine and Public Health Flinders University Bedford Park South Australia AustraliaABSTRACT Extracellular vesicles (EVs) are nanosized, membrane‐bound particles released by virtually all cell types, serving as messengers within tissues and across organs via the bloodstream. EVs encapsulate diverse molecular cargo that reflects the phenotypic state of their originating cells, making them promising candidates for liquid biopsy applications. However, the heterogeneity of circulating EVs, comprising particles from various cell types and non‐vesicular entities like lipoproteins, poses significant challenges for isolating tissue‐specific EV populations. This review examines current methodologies for detecting and isolating tissue‐specific EVs from blood, focusing on immunoaffinity capture (IAC) strategies that leverage surface marker expression for specificity. Key considerations, including the selection and validation of markers, are discussed alongside advances in EV subtyping and isolation protocols. Challenges such as marker cross‐reactivity, EV biogenesis and transport dynamics are highlighted to underscore the complexity of achieving clinical utility. By providing an overview of validated tissue‐specific markers and isolation techniques, this review aims to facilitate the development of EV‐based biomarkers with enhanced specificity and sensitivity, enabling minimally invasive monitoring of organ function and disease.https://doi.org/10.1002/jex2.70059cell originEV subtypesextracellular vesiclesimmunoaffinity isolationorgan‐derivedtissue‐specific |
| spellingShingle | Lauren Newman Andrew Rowland Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood Journal of Extracellular Biology cell origin EV subtypes extracellular vesicles immunoaffinity isolation organ‐derived tissue‐specific |
| title | Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood |
| title_full | Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood |
| title_fullStr | Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood |
| title_full_unstemmed | Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood |
| title_short | Detection and Isolation of Tissue‐Specific Extracellular Vesicles From the Blood |
| title_sort | detection and isolation of tissue specific extracellular vesicles from the blood |
| topic | cell origin EV subtypes extracellular vesicles immunoaffinity isolation organ‐derived tissue‐specific |
| url | https://doi.org/10.1002/jex2.70059 |
| work_keys_str_mv | AT laurennewman detectionandisolationoftissuespecificextracellularvesiclesfromtheblood AT andrewrowland detectionandisolationoftissuespecificextracellularvesiclesfromtheblood |