Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses

In order to counteract harmful effects of oxidative stress due to pathological conditions or physical exercise, horses are often administered dietary supplements having supposed high antioxidant activities. The aim of the present study was to identify the in vitro antioxidant potential of “ImmuPlus”...

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Main Authors: Stefano Cecchini, Mariateresa Paciolla, Anna Rocchina Caputo, Alfonso Bavoso
Format: Article
Language:English
Published: Wiley 2014-01-01
Series:Veterinary Medicine International
Online Access:http://dx.doi.org/10.1155/2014/434239
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author Stefano Cecchini
Mariateresa Paciolla
Anna Rocchina Caputo
Alfonso Bavoso
author_facet Stefano Cecchini
Mariateresa Paciolla
Anna Rocchina Caputo
Alfonso Bavoso
author_sort Stefano Cecchini
collection DOAJ
description In order to counteract harmful effects of oxidative stress due to pathological conditions or physical exercise, horses are often administered dietary supplements having supposed high antioxidant activities. The aim of the present study was to identify the in vitro antioxidant potential of “ImmuPlus”, a polyherbal formulation (Global Herbs LTD, Chichester, West Sussex, Great Britain), containing three medicinal plants (Withania somnifera, Tinospora cordifolia, and Emblica officinalis), known in Ayurveda for their use in human disease treatment. Extracts obtained by different solvents (water, methanol, ethanol, acetone, and hexane) were tested for total antioxidant capacity, total reducing power, scavenging activity against DPPH radical, and total polyphenol and flavonoid contents. Our results showed that, except as regards hexane, all the used solvents are able to extract compounds having high antioxidant activity, even when compared to ascorbic acid. Regression analysis showed significant correlations between antioxidant properties and polyphenol/flavonoid contents, indicating the latter, known for their beneficial effects on health of human and animal beings, as major components responsible for the strong antioxidant capacities. Moreover, obtained results suggest the effective role of the polyherbal mixture as good source of antioxidants in horses.
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series Veterinary Medicine International
spelling doaj-art-dd4e290912d84fb3933427a8b3c321bc2025-08-20T03:26:29ZengWileyVeterinary Medicine International2090-81132042-00482014-01-01201410.1155/2014/434239434239Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for HorsesStefano Cecchini0Mariateresa Paciolla1Anna Rocchina Caputo2Alfonso Bavoso3Dipartimento di Scienze, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyDipartimento di Scienze, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyConsiglio per la Ricerca e la Sperimentazione in Agricoltura-Unità di Ricerca per la Zootecnia Estensiva, 85054 Muro Lucano, ItalyDipartimento di Scienze, Università degli Studi della Basilicata, Viale dell’Ateneo Lucano 10, 85100 Potenza, ItalyIn order to counteract harmful effects of oxidative stress due to pathological conditions or physical exercise, horses are often administered dietary supplements having supposed high antioxidant activities. The aim of the present study was to identify the in vitro antioxidant potential of “ImmuPlus”, a polyherbal formulation (Global Herbs LTD, Chichester, West Sussex, Great Britain), containing three medicinal plants (Withania somnifera, Tinospora cordifolia, and Emblica officinalis), known in Ayurveda for their use in human disease treatment. Extracts obtained by different solvents (water, methanol, ethanol, acetone, and hexane) were tested for total antioxidant capacity, total reducing power, scavenging activity against DPPH radical, and total polyphenol and flavonoid contents. Our results showed that, except as regards hexane, all the used solvents are able to extract compounds having high antioxidant activity, even when compared to ascorbic acid. Regression analysis showed significant correlations between antioxidant properties and polyphenol/flavonoid contents, indicating the latter, known for their beneficial effects on health of human and animal beings, as major components responsible for the strong antioxidant capacities. Moreover, obtained results suggest the effective role of the polyherbal mixture as good source of antioxidants in horses.http://dx.doi.org/10.1155/2014/434239
spellingShingle Stefano Cecchini
Mariateresa Paciolla
Anna Rocchina Caputo
Alfonso Bavoso
Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses
Veterinary Medicine International
title Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses
title_full Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses
title_fullStr Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses
title_full_unstemmed Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses
title_short Antioxidant Potential of the Polyherbal Formulation “ImmuPlus”: A Nutritional Supplement for Horses
title_sort antioxidant potential of the polyherbal formulation immuplus a nutritional supplement for horses
url http://dx.doi.org/10.1155/2014/434239
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AT annarocchinacaputo antioxidantpotentialofthepolyherbalformulationimmuplusanutritionalsupplementforhorses
AT alfonsobavoso antioxidantpotentialofthepolyherbalformulationimmuplusanutritionalsupplementforhorses