Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis
Background The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.Materials and methods PRF membranes and liquid-PRF were prepared usi...
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Taylor & Francis Group
2025-12-01
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| Series: | Journal of Oral Microbiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20002297.2024.2445598 |
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| author | Fabio Hideaki Uyeda Gustavo Quilles Vargas Larissa Matias Malavazi Tatiane Tiemi Macedo Aline Paim de Abreu Paulo Gomes Manuela Rocha Bueno Pedro Henrique Moreira Paulo Tolentino Lucas Daylor Aguiar da Silva Luciene Cristina Figueiredo Jamil Awad Shibli Bruno Bueno-Silva |
| author_facet | Fabio Hideaki Uyeda Gustavo Quilles Vargas Larissa Matias Malavazi Tatiane Tiemi Macedo Aline Paim de Abreu Paulo Gomes Manuela Rocha Bueno Pedro Henrique Moreira Paulo Tolentino Lucas Daylor Aguiar da Silva Luciene Cristina Figueiredo Jamil Awad Shibli Bruno Bueno-Silva |
| author_sort | Fabio Hideaki Uyeda |
| collection | DOAJ |
| description | Background The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.Materials and methods PRF membranes and liquid-PRF were prepared using two protocols: centrifuge 1 uses fixed acceleration while centrifuge 2, progressive acceleration. PRF samples were introduced into device concurrently with 33-species bacterial inoculum. After seven days, biofilm metabolic activity (MA) and microbial profile were evaluated through colorimetric reaction and DNA–DNA hybridization, respectively.Results Among PRF membranes, the ones from centrifuge 1 led to better reduction in MA, total biofilm, and F. periodonticum, P. gingivalis and T. forsythia counts when compared to untreated/centrifuge 2 treated biofilms. However, centrifuge 2 liquid-PRF reduced MA, total biofilm and F. periodonticum counts when compared to untreated/centrifuge 1 treated-biofilms.Conclusion PRF membrane and exhibited comparable antibiofilm activity. However, PRF distinct forms, obtained by same centrifugation protocol, may present different antimicrobial properties. |
| format | Article |
| id | doaj-art-dd457d9a67384792aaf7c6d154b9e66a |
| institution | Kabale University |
| issn | 2000-2297 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Oral Microbiology |
| spelling | doaj-art-dd457d9a67384792aaf7c6d154b9e66a2025-01-30T05:46:57ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972025-12-0117110.1080/20002297.2024.2445598Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitisFabio Hideaki Uyeda0Gustavo Quilles Vargas1Larissa Matias Malavazi2Tatiane Tiemi Macedo3Aline Paim de Abreu Paulo Gomes4Manuela Rocha Bueno5Pedro Henrique Moreira Paulo Tolentino6Lucas Daylor Aguiar da Silva7Luciene Cristina Figueiredo8Jamil Awad Shibli9Bruno Bueno-Silva10Dental Research Division, Guarulhos University, Guarulhos, BrazilDepartment of Biosciences; Piracicaba School of Dentistry, University of Campinas, Piracicaba, BrazilDepartment of Biosciences; Piracicaba School of Dentistry, University of Campinas, Piracicaba, BrazilDental Research Division, Guarulhos University, Guarulhos, BrazilDental Research Division, Guarulhos University, Guarulhos, BrazilDepartment of Periodontology, São Leopoldo Mandic Faculty, Campinas, BrazilDental Research Division, Guarulhos University, Guarulhos, BrazilDental Research Division, Guarulhos University, Guarulhos, BrazilDental Research Division, Guarulhos University, Guarulhos, BrazilDental Research Division, Guarulhos University, Guarulhos, BrazilDepartment of Biosciences; Piracicaba School of Dentistry, University of Campinas, Piracicaba, BrazilBackground The aim of this article is to evaluate the effect of different portions of Platelet Rich Fibrin (PRF) membranes and liquid-PRF, prepared by two distinct protocols/centrifuges each, on the multispecies subgingival biofilm.Materials and methods PRF membranes and liquid-PRF were prepared using two protocols: centrifuge 1 uses fixed acceleration while centrifuge 2, progressive acceleration. PRF samples were introduced into device concurrently with 33-species bacterial inoculum. After seven days, biofilm metabolic activity (MA) and microbial profile were evaluated through colorimetric reaction and DNA–DNA hybridization, respectively.Results Among PRF membranes, the ones from centrifuge 1 led to better reduction in MA, total biofilm, and F. periodonticum, P. gingivalis and T. forsythia counts when compared to untreated/centrifuge 2 treated biofilms. However, centrifuge 2 liquid-PRF reduced MA, total biofilm and F. periodonticum counts when compared to untreated/centrifuge 1 treated-biofilms.Conclusion PRF membrane and exhibited comparable antibiofilm activity. However, PRF distinct forms, obtained by same centrifugation protocol, may present different antimicrobial properties.https://www.tandfonline.com/doi/10.1080/20002297.2024.2445598Subgingival biofilmperiodontitisantimicrobialplatelet-rich fibriniPRF |
| spellingShingle | Fabio Hideaki Uyeda Gustavo Quilles Vargas Larissa Matias Malavazi Tatiane Tiemi Macedo Aline Paim de Abreu Paulo Gomes Manuela Rocha Bueno Pedro Henrique Moreira Paulo Tolentino Lucas Daylor Aguiar da Silva Luciene Cristina Figueiredo Jamil Awad Shibli Bruno Bueno-Silva Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis Journal of Oral Microbiology Subgingival biofilm periodontitis antimicrobial platelet-rich fibrin iPRF |
| title | Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis |
| title_full | Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis |
| title_fullStr | Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis |
| title_full_unstemmed | Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis |
| title_short | Platelet-rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis |
| title_sort | platelet rich fibrin obtained from different protocols affects the formation of the in vitro multispecies subgingival biofilm associated with periodontitis |
| topic | Subgingival biofilm periodontitis antimicrobial platelet-rich fibrin iPRF |
| url | https://www.tandfonline.com/doi/10.1080/20002297.2024.2445598 |
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