Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui
Abstract Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward CO2 redu...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-53946-3 |
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| _version_ | 1849221116044771328 |
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| author | Weisong Liu Kuncheng Zhang Jiang Liu Yuanming Wang Meng Zhang Huijuan Cui Junsong Sun Lingling Zhang |
| author_facet | Weisong Liu Kuncheng Zhang Jiang Liu Yuanming Wang Meng Zhang Huijuan Cui Junsong Sun Lingling Zhang |
| author_sort | Weisong Liu |
| collection | DOAJ |
| description | Abstract Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward CO2 reduction. Herein, we engineer TkHDCR to generate an electro-responsive carbon dioxide reductase considering the safety and convenience. To achieve this purpose, a recombinant Escherichia coli TkHDCR overexpression system is established. The formate dehydrogenase is obtained via subunit truncation and rational design, which enables direct electron transfer (DET)-type bioelectrocatalysis with a near-zero overpotential. By applying a constant voltage of −500 mV (vs. SHE) to a mediated electrolytic cell, 22.8 ± 1.6 mM formate is synthesized in 16 h with an average production rate of 7.1 ± 0.5 μmol h−1cm−2, a Faradaic efficiency of 98.9% and a half-cell energy efficiency of 94.4%. This study provides an enzyme candidate for high efficient CO2 reduction and opens up a way to develop paradigm for CO2-based bio-manufacturing. |
| format | Article |
| id | doaj-art-dd3ea099b03a4a81b3fd394791163a98 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-dd3ea099b03a4a81b3fd394791163a982024-11-24T12:32:50ZengNature PortfolioNature Communications2041-17232024-11-0115111110.1038/s41467-024-53946-3Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivuiWeisong Liu0Kuncheng Zhang1Jiang Liu2Yuanming Wang3Meng Zhang4Huijuan Cui5Junsong Sun6Lingling Zhang7Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 west 7th Avenue, Tianjin Airport Economic AreaKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 west 7th Avenue, Tianjin Airport Economic AreaIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesIn vitro Synthetic Biology Center, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 west 7th Avenue, Tianjin Airport Economic AreaUniversity of Chinese Academy of SciencesKey Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, 32 west 7th Avenue, Tianjin Airport Economic AreaAbstract Electrocatalytic carbon dioxide (CO2) reduction by CO2 reductases is a promising approach for biomanufacturing. Among all known biological or chemical catalysts, hydrogen-dependent carbon dioxide reductase from Thermoanaerobacter kivui (TkHDCR) possesses the highest activity toward CO2 reduction. Herein, we engineer TkHDCR to generate an electro-responsive carbon dioxide reductase considering the safety and convenience. To achieve this purpose, a recombinant Escherichia coli TkHDCR overexpression system is established. The formate dehydrogenase is obtained via subunit truncation and rational design, which enables direct electron transfer (DET)-type bioelectrocatalysis with a near-zero overpotential. By applying a constant voltage of −500 mV (vs. SHE) to a mediated electrolytic cell, 22.8 ± 1.6 mM formate is synthesized in 16 h with an average production rate of 7.1 ± 0.5 μmol h−1cm−2, a Faradaic efficiency of 98.9% and a half-cell energy efficiency of 94.4%. This study provides an enzyme candidate for high efficient CO2 reduction and opens up a way to develop paradigm for CO2-based bio-manufacturing.https://doi.org/10.1038/s41467-024-53946-3 |
| spellingShingle | Weisong Liu Kuncheng Zhang Jiang Liu Yuanming Wang Meng Zhang Huijuan Cui Junsong Sun Lingling Zhang Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui Nature Communications |
| title | Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui |
| title_full | Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui |
| title_fullStr | Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui |
| title_full_unstemmed | Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui |
| title_short | Bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from Thermoanaerobacter kivui |
| title_sort | bioelectrocatalytic carbon dioxide reduction by an engineered formate dehydrogenase from thermoanaerobacter kivui |
| url | https://doi.org/10.1038/s41467-024-53946-3 |
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