Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity
IntroductionThe global health challenge of ulcerative colitis (UC) has been classified by the WHO as a modern refractory disease, commonly referred to as green cancer, with limited treatment options still available, highlighting the urgent need for the development of new therapeutic strategies. Rece...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1528578/full |
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| author | Wen-lin Pang Wen-lin Pang Tian-gang Li Tian-gang Li Yin-ying Wang Yin-ying Wang Li-yun Song Li-yun Song Li Li Li Li Xiao-ya Li Xiao-ya Li Yong Qiu Yong Qiu Zhong-shan Yang Zhong-shan Yang |
| author_facet | Wen-lin Pang Wen-lin Pang Tian-gang Li Tian-gang Li Yin-ying Wang Yin-ying Wang Li-yun Song Li-yun Song Li Li Li Li Xiao-ya Li Xiao-ya Li Yong Qiu Yong Qiu Zhong-shan Yang Zhong-shan Yang |
| author_sort | Wen-lin Pang |
| collection | DOAJ |
| description | IntroductionThe global health challenge of ulcerative colitis (UC) has been classified by the WHO as a modern refractory disease, commonly referred to as green cancer, with limited treatment options still available, highlighting the urgent need for the development of new therapeutic strategies. Recent pharmacological research has shown that traditional Chinese medicine saussurea costus (SC) possesses beneficial antibacterial and anti-inflammatory properties. Nevertheless, its underlying mechanism remains elusive.MethodsFirstly, we identified the main active components of SC through UHPLC-QTOF-MS analysis. Subsequently, UC mice were induced using DSS and administered different doses of SC to evaluate its efficacy. Additionally, the impact of SC on the repair of the intestinal mucosal barrier was evaluated through immunofluorescence and western blot. Furthermore, 16s rRNA gene sequencing was conducted to elucidate the contribution of gut microbiota to UC pathogenesis.ResultsThe primary components of SC include Proline, Phenylalanine, Isoleucine, Lucidenic acid M, and Pyroglutamic acid. The efficacy of SC was concurrently assessed, revealing its potential to ameliorate histological injury in colitis mice. Furthermore, SC was found to decrease levels of TNF-α, IL-1β, IL-8, and IL-18 while promoting the expression of IL-10 and IL-22. Similarly, we also found that the expression of ZO-1 and Occludin was reversed by SC in colitis mice. In addition, analysis of 16S rRNA gene sequencing indicated that SC reduced harmful bacterial populations, such as Proteobacteria, while simultaneously enhancing the levels of beneficial bacteria like Lactobacillus, thereby contributing to the improvement of UC pathology.ConclusionThis study highlights the therapeutic potential of SC in managing UC through its ability to attenuate inflammatory responses, restore intestinal barrier functionality, and modulate gut microbiota composition, which findings offer insights into potential strategies for advancing UC treatment. |
| format | Article |
| id | doaj-art-dd2631d7bbc34dcf821606acba44c7c8 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-dd2631d7bbc34dcf821606acba44c7c82025-01-28T06:41:18ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-01-011510.3389/fcimb.2025.15285781528578Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrityWen-lin Pang0Wen-lin Pang1Tian-gang Li2Tian-gang Li3Yin-ying Wang4Yin-ying Wang5Li-yun Song6Li-yun Song7Li Li8Li Li9Xiao-ya Li10Xiao-ya Li11Yong Qiu12Yong Qiu13Zhong-shan Yang14Zhong-shan Yang15Yunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaZhongshan Hospital of Traditional Chinese Medicine, Affiliated to Guangzhou University of Chinese Medicine, Zhongshan, Guangdong, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaYunnan Provincial Key Laboratory of Integrated Traditional Chinese and Western Medicine for Chronic Disease in Prevention and Treatment, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan, ChinaYunnan Provincial Department of Education, Engineering Research Center of Classic Formula Regulate Immunity in Chronic Disease Prevention and Treatment, Kunming, Yunnan, ChinaIntroductionThe global health challenge of ulcerative colitis (UC) has been classified by the WHO as a modern refractory disease, commonly referred to as green cancer, with limited treatment options still available, highlighting the urgent need for the development of new therapeutic strategies. Recent pharmacological research has shown that traditional Chinese medicine saussurea costus (SC) possesses beneficial antibacterial and anti-inflammatory properties. Nevertheless, its underlying mechanism remains elusive.MethodsFirstly, we identified the main active components of SC through UHPLC-QTOF-MS analysis. Subsequently, UC mice were induced using DSS and administered different doses of SC to evaluate its efficacy. Additionally, the impact of SC on the repair of the intestinal mucosal barrier was evaluated through immunofluorescence and western blot. Furthermore, 16s rRNA gene sequencing was conducted to elucidate the contribution of gut microbiota to UC pathogenesis.ResultsThe primary components of SC include Proline, Phenylalanine, Isoleucine, Lucidenic acid M, and Pyroglutamic acid. The efficacy of SC was concurrently assessed, revealing its potential to ameliorate histological injury in colitis mice. Furthermore, SC was found to decrease levels of TNF-α, IL-1β, IL-8, and IL-18 while promoting the expression of IL-10 and IL-22. Similarly, we also found that the expression of ZO-1 and Occludin was reversed by SC in colitis mice. In addition, analysis of 16S rRNA gene sequencing indicated that SC reduced harmful bacterial populations, such as Proteobacteria, while simultaneously enhancing the levels of beneficial bacteria like Lactobacillus, thereby contributing to the improvement of UC pathology.ConclusionThis study highlights the therapeutic potential of SC in managing UC through its ability to attenuate inflammatory responses, restore intestinal barrier functionality, and modulate gut microbiota composition, which findings offer insights into potential strategies for advancing UC treatment.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1528578/fullgut microbiotaintestinal barrierinflammationsaussurea costusulcerative colitis |
| spellingShingle | Wen-lin Pang Wen-lin Pang Tian-gang Li Tian-gang Li Yin-ying Wang Yin-ying Wang Li-yun Song Li-yun Song Li Li Li Li Xiao-ya Li Xiao-ya Li Yong Qiu Yong Qiu Zhong-shan Yang Zhong-shan Yang Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity Frontiers in Cellular and Infection Microbiology gut microbiota intestinal barrier inflammation saussurea costus ulcerative colitis |
| title | Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity |
| title_full | Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity |
| title_fullStr | Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity |
| title_full_unstemmed | Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity |
| title_short | Saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity |
| title_sort | saussurea costus alleviates ulcerative colitis by regulating the gut microbiota and improving intestinal barrier integrity |
| topic | gut microbiota intestinal barrier inflammation saussurea costus ulcerative colitis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1528578/full |
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