Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam
In this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1T could highly tolerate Cu2+ up to 10 mM, and the isolates could also grow in a wide range of pH (3–7), and temperature (10–40 °C). Dried bi...
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| Format: | Article |
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Taylor & Francis Group
2023-11-01
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| Series: | Mycobiology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2023.2274648 |
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| author | Kim Cuc Thi Nguyen Phuc Hung Truong Cuong Tu Ho Cong Tuan Le Khoa Dang Tran Tien Long Nguyen Manh Tuan Nguyen Phu Van Nguyen |
| author_facet | Kim Cuc Thi Nguyen Phuc Hung Truong Cuong Tu Ho Cong Tuan Le Khoa Dang Tran Tien Long Nguyen Manh Tuan Nguyen Phu Van Nguyen |
| author_sort | Kim Cuc Thi Nguyen |
| collection | DOAJ |
| description | In this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1T could highly tolerate Cu2+ up to 10 mM, and the isolates could also grow in a wide range of pH (3–7), and temperature (10–40 °C). Dried biomass of GL1 was able to remove Cu2+ effectively up to 90.49% with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 °C, and incubation time 60 min. Sequence analysis of rDNA indicated this strain was closely related to Rhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2 domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respectively. Based on phylogenetic tree analysis and the biochemical characteristics, the strain appears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is proposed. This study provides us with more information about heavy metal-tolerant yeasts and it may produce a new tool for environmental control and metal recovery operations. |
| format | Article |
| id | doaj-art-dd2436e608c8429fbb44bce772ea1ef1 |
| institution | Kabale University |
| issn | 1229-8093 2092-9323 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj-art-dd2436e608c8429fbb44bce772ea1ef12025-08-20T03:58:11ZengTaylor & Francis GroupMycobiology1229-80932092-93232023-11-0151637938710.1080/12298093.2023.2274648Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, VietnamKim Cuc Thi Nguyen0Phuc Hung Truong1Cuong Tu Ho2Cong Tuan Le3Khoa Dang Tran4Tien Long Nguyen5Manh Tuan Nguyen6Phu Van Nguyen7Institute of Biotechnology, Hue University, Thua Thien Hue, VietnamFaculty of Biotechnology, TNU- University of Sciences, Thai Nguyen City, VietnamInstitute of Environmental Technology, Vietnam Academy of Science and Technology, Hanoi, VietnamDepartment of Environmental Science, University of Sciences, Hue University, Thua Thien Hue, VietnamFaculty of Agronomy, University of Agriculture and Forestry, Hue University, Thua Thien Hue, VietnamDepartment of Educational Management, University of Agriculture and Forestry, Hue University, Thua Thien Hue, VietnamInstitute of Life Science, Thai Nguyen University of Agriculture and Forestry, Thai Nguyen City, VietnamInstitute of Biotechnology, Hue University, Thua Thien Hue, VietnamIn this study, twenty-five yeast strains were isolated from soil samples collected in the gold mining ore in Gia Lai, Vietnam. Among them, one isolate named GL1T could highly tolerate Cu2+ up to 10 mM, and the isolates could also grow in a wide range of pH (3–7), and temperature (10–40 °C). Dried biomass of GL1 was able to remove Cu2+ effectively up to 90.49% with a maximal biosorption capacity of 18.1 mg/g at pH 6, temperature 30 °C, and incubation time 60 min. Sequence analysis of rDNA indicated this strain was closely related to Rhodotorula mucilaginosa but with 1.53 and 3.46% nucleotide differences in the D1/D2 domain of the 28S rRNA gene and the ITS1-5.8S rRNA gene-ITS2 region sequence, respectively. Based on phylogenetic tree analysis and the biochemical characteristics, the strain appears to be a novel Rhodotorula species, and the name Rhodotorula aurum sp. nov. is proposed. This study provides us with more information about heavy metal-tolerant yeasts and it may produce a new tool for environmental control and metal recovery operations.https://www.tandfonline.com/doi/10.1080/12298093.2023.2274648Biosorptioncopper-tolerant yeastRhodotorulacopper stressgold mining |
| spellingShingle | Kim Cuc Thi Nguyen Phuc Hung Truong Cuong Tu Ho Cong Tuan Le Khoa Dang Tran Tien Long Nguyen Manh Tuan Nguyen Phu Van Nguyen Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam Mycobiology Biosorption copper-tolerant yeast Rhodotorula copper stress gold mining |
| title | Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam |
| title_full | Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam |
| title_fullStr | Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam |
| title_full_unstemmed | Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam |
| title_short | Copper Tolerance of Novel Rhodotorula sp. Yeast Isolated from Gold Mining Ore in Gia Lai, Vietnam |
| title_sort | copper tolerance of novel rhodotorula sp yeast isolated from gold mining ore in gia lai vietnam |
| topic | Biosorption copper-tolerant yeast Rhodotorula copper stress gold mining |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2023.2274648 |
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