In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA
Background: Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) are significant pathogens responsible for a range of infections, including those in diabetic patients. The increasing resistance to antibiotics necessitates the exploration of altern...
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| Format: | Article |
| Language: | English |
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AOSIS
2025-05-01
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| Series: | Journal of Medicinal Plants for Economic Development |
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| Online Access: | https://jomped.org/index.php/jomped/article/view/280 |
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| author | Nhlanhla W. Nsele Kegomoditswe P. Mathobela Siyabonga P. Radebe Nokukhanya Thembane |
| author_facet | Nhlanhla W. Nsele Kegomoditswe P. Mathobela Siyabonga P. Radebe Nokukhanya Thembane |
| author_sort | Nhlanhla W. Nsele |
| collection | DOAJ |
| description | Background: Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) are significant pathogens responsible for a range of infections, including those in diabetic patients. The increasing resistance to antibiotics necessitates the exploration of alternative antibacterial agents. The Psidium guajava (guava) plant has demonstrated antimicrobial properties, but its efficacy against S. aureus strains remains underexplored.
Aim: To evaluate the in vitro antibacterial activity of ethanol and water extracts of guava leaf extracts against MRSA and MSSA.
Setting: In vitro study conducted under controlled laboratory settings at Mangosuthu University of Technology, Department of Biomedical Sciences, Kwa-Zulu Natal, South Africa.
Methods: Guava leaf extracts were prepared using ethanol and water. Antibacterial activity was assessed using the Kirby-Bauer disc diffusion method and minimum inhibitory concentration (MIC) assays. The zone of inhibition size and MIC values were measured for both MRSA and MSSA.
Results: The water-based extract produced larger zones of inhibition and lower MIC values compared to the ethanol extract, indicating higher potency. However, both extracts showed reduced activity against MRSA, which may be attributed to the presence of the mecA gene, conferring resistance to β-lactam antibiotics.
Conclusion: While the water-based guava extract demonstrated significant antibacterial potential against both MRSA and MSSA, further research is needed to isolate specific bioactive compounds and assess clinical applicability.
Contribution: These findings suggest that guava leaves may offer a natural alternative or adjunctive treatment for S. aureus infections, including those caused by antibiotic-resistant strains. |
| format | Article |
| id | doaj-art-dd219042f013424f8d6809fcc5e59e17 |
| institution | DOAJ |
| issn | 2519-559X 2616-4809 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | AOSIS |
| record_format | Article |
| series | Journal of Medicinal Plants for Economic Development |
| spelling | doaj-art-dd219042f013424f8d6809fcc5e59e172025-08-20T03:07:34ZengAOSISJournal of Medicinal Plants for Economic Development2519-559X2616-48092025-05-0191e1e710.4102/jomped.v9i1.280125In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSANhlanhla W. Nsele0Kegomoditswe P. Mathobela1Siyabonga P. Radebe2Nokukhanya Thembane3Department of Biomedical Sciences, Faculty of Applied and Health Sciences, Mangosuthu University of Technology, DurbanDepartment of Biomedical Sciences, Faculty of Applied and Health Sciences, Mangosuthu University of Technology, DurbanDepartment of Biomedical Sciences, Faculty of Applied and Health Sciences, Mangosuthu University of Technology, DurbanDepartment of Biomedical Sciences, Faculty of Applied and Health Sciences, Mangosuthu University of Technology, DurbanBackground: Methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) are significant pathogens responsible for a range of infections, including those in diabetic patients. The increasing resistance to antibiotics necessitates the exploration of alternative antibacterial agents. The Psidium guajava (guava) plant has demonstrated antimicrobial properties, but its efficacy against S. aureus strains remains underexplored. Aim: To evaluate the in vitro antibacterial activity of ethanol and water extracts of guava leaf extracts against MRSA and MSSA. Setting: In vitro study conducted under controlled laboratory settings at Mangosuthu University of Technology, Department of Biomedical Sciences, Kwa-Zulu Natal, South Africa. Methods: Guava leaf extracts were prepared using ethanol and water. Antibacterial activity was assessed using the Kirby-Bauer disc diffusion method and minimum inhibitory concentration (MIC) assays. The zone of inhibition size and MIC values were measured for both MRSA and MSSA. Results: The water-based extract produced larger zones of inhibition and lower MIC values compared to the ethanol extract, indicating higher potency. However, both extracts showed reduced activity against MRSA, which may be attributed to the presence of the mecA gene, conferring resistance to β-lactam antibiotics. Conclusion: While the water-based guava extract demonstrated significant antibacterial potential against both MRSA and MSSA, further research is needed to isolate specific bioactive compounds and assess clinical applicability. Contribution: These findings suggest that guava leaves may offer a natural alternative or adjunctive treatment for S. aureus infections, including those caused by antibiotic-resistant strains.https://jomped.org/index.php/jomped/article/view/280guavatype 2 diabetesmethicillin-resistant staphylococcus aureusmethicillin-sensitive staphylococcus aureusantimicrobial resistance |
| spellingShingle | Nhlanhla W. Nsele Kegomoditswe P. Mathobela Siyabonga P. Radebe Nokukhanya Thembane In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA Journal of Medicinal Plants for Economic Development guava type 2 diabetes methicillin-resistant staphylococcus aureus methicillin-sensitive staphylococcus aureus antimicrobial resistance |
| title | In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA |
| title_full | In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA |
| title_fullStr | In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA |
| title_full_unstemmed | In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA |
| title_short | In vitro antibacterial activity of Psidium guajava (guava) extracts against MRSA and MSSA |
| title_sort | in vitro antibacterial activity of psidium guajava guava extracts against mrsa and mssa |
| topic | guava type 2 diabetes methicillin-resistant staphylococcus aureus methicillin-sensitive staphylococcus aureus antimicrobial resistance |
| url | https://jomped.org/index.php/jomped/article/view/280 |
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