Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry
Gelatin from deer has garnered attention as a high-value health-promoting resource given its history of usage as a traditional Chinese medicine and recent studies demonstrating its biological activities. Mass spectrometry-based methods have increasingly been employed for species identification in co...
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MDPI AG
2025-03-01
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| author | Kouharu Otsuki Aya Nomizo Mi Zhang Dongxia Li Takashi Kikuchi Wei Li |
| author_facet | Kouharu Otsuki Aya Nomizo Mi Zhang Dongxia Li Takashi Kikuchi Wei Li |
| author_sort | Kouharu Otsuki |
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| description | Gelatin from deer has garnered attention as a high-value health-promoting resource given its history of usage as a traditional Chinese medicine and recent studies demonstrating its biological activities. Mass spectrometry-based methods have increasingly been employed for species identification in collagen-based materials, effectively addressing challenges in quality control and authenticity verification. This study aims to identify characteristic marker peptides in gelatins from sika deer (<i>Cervus nippon</i>) to support their effective use as a health-promoting resource. Gelatin samples were enzymatically digested, and the resulting peptide mixtures were analyzed using ultra-high-performance liquid chromatography coupled with quadrupole Q-Exactive-Orbitrap mass spectrometry (UHPLC-Q-Exactive-Orbitrap MS). Marker peptide candidates were selected based on their high detection intensity and a literature review. Among the 28 selected marker peptide candidates, four peptides (P11, R2, R3, and R4) were defined as characteristic of sika deer gelatin. Comparative analyses with gelatins derived from donkey hide, bovine, porcine, and fish samples further confirmed the specificity of these peptides. These findings establish a robust analytical method for verifying the authenticity of sika deer gelatin, contributing to its safe and effective use as a health-promoting resource. |
| format | Article |
| id | doaj-art-dd13ced07f5f413aa8a312eea887068f |
| institution | DOAJ |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-03-01 |
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| series | Molecules |
| spelling | doaj-art-dd13ced07f5f413aa8a312eea887068f2025-08-20T03:03:28ZengMDPI AGMolecules1420-30492025-03-01307152810.3390/molecules30071528Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass SpectrometryKouharu Otsuki0Aya Nomizo1Mi Zhang2Dongxia Li3Takashi Kikuchi4Wei Li5Faculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, JapanDepartment of Medical Laboratory, Medical College of Dalian University, Dalian 116622, ChinaFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, JapanFaculty of Pharmaceutical Sciences, Toho University, Miyama 2-2-1, Funabashi 274-8510, JapanGelatin from deer has garnered attention as a high-value health-promoting resource given its history of usage as a traditional Chinese medicine and recent studies demonstrating its biological activities. Mass spectrometry-based methods have increasingly been employed for species identification in collagen-based materials, effectively addressing challenges in quality control and authenticity verification. This study aims to identify characteristic marker peptides in gelatins from sika deer (<i>Cervus nippon</i>) to support their effective use as a health-promoting resource. Gelatin samples were enzymatically digested, and the resulting peptide mixtures were analyzed using ultra-high-performance liquid chromatography coupled with quadrupole Q-Exactive-Orbitrap mass spectrometry (UHPLC-Q-Exactive-Orbitrap MS). Marker peptide candidates were selected based on their high detection intensity and a literature review. Among the 28 selected marker peptide candidates, four peptides (P11, R2, R3, and R4) were defined as characteristic of sika deer gelatin. Comparative analyses with gelatins derived from donkey hide, bovine, porcine, and fish samples further confirmed the specificity of these peptides. These findings establish a robust analytical method for verifying the authenticity of sika deer gelatin, contributing to its safe and effective use as a health-promoting resource.https://www.mdpi.com/1420-3049/30/7/1528sika deergelatinmarker peptideLC-MS/MS |
| spellingShingle | Kouharu Otsuki Aya Nomizo Mi Zhang Dongxia Li Takashi Kikuchi Wei Li Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry Molecules sika deer gelatin marker peptide LC-MS/MS |
| title | Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry |
| title_full | Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry |
| title_fullStr | Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry |
| title_full_unstemmed | Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry |
| title_short | Identification of Marker Peptides in Gelatins from Sika Deer (<i>Cervus nippon</i>) Using Ultra-High-Performance Liquid Chromatography–Quadrupole-Exactive-Orbitrap Mass Spectrometry |
| title_sort | identification of marker peptides in gelatins from sika deer i cervus nippon i using ultra high performance liquid chromatography quadrupole exactive orbitrap mass spectrometry |
| topic | sika deer gelatin marker peptide LC-MS/MS |
| url | https://www.mdpi.com/1420-3049/30/7/1528 |
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