Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study
Abstract Background Areca nut (AN) was classified as a carcinogen by the International Agency for Research on Cancer (IARC) of the WHO in 2003. AN has the same carcinogenic components as cigarettes, such as benzo[a]pyrene (B[a]P) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), but its effe...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-04-01
|
| Series: | BMC Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12885-025-14020-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849737675359125504 |
|---|---|
| author | Yixuan Li Jing Zhou Lirong Liu Chaoyong Zhu Ziyue Luo Na Li Pengfei Lyu Jing Zhang Tian Xie Yipeng Ding Sha Xiao |
| author_facet | Yixuan Li Jing Zhou Lirong Liu Chaoyong Zhu Ziyue Luo Na Li Pengfei Lyu Jing Zhang Tian Xie Yipeng Ding Sha Xiao |
| author_sort | Yixuan Li |
| collection | DOAJ |
| description | Abstract Background Areca nut (AN) was classified as a carcinogen by the International Agency for Research on Cancer (IARC) of the WHO in 2003. AN has the same carcinogenic components as cigarettes, such as benzo[a]pyrene (B[a]P) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), but its effects on interactions with genetic factors related to lung cancer have rarely been investigated. Methods Here, a propensity score-matched case‒control study was conducted in Hainan, which included 445 patients with lung cancer and 445 cancer-free controls. Then, the associations between single-nucleotide polymorphisms (SNPs) in the CHRNA5-CHRNA3-CHRNB4 gene cluster and their interaction effects with AN chewing and smoking on lung cancer were analyzed. In addition, we explored the associations among AN, cigarettes, and genes related to lung cancer using the Comparative Toxicogenomics Database (CTD). Results The results indicate that the CHRNA3 rs938682 (A > G) GG genotype (OR = 0.669, 95% CI = 0.454 ~ 0.984, P = 0.042) can decrease the risk of lung cancer. The CHRNB4 rs7178270 (C > G) GG genotype (OR = 1.729, 95% CI = 1.168 ~ 2.571, P = 0.006) can increase the risk of lung cancer. The CHRNA5 rs17486278 CC genotype was associated with a high risk in males, smokers, and drinkers. The CHRNA3 rs938682 GG genotype was associated with a low risk in AN chewers. The CHRNB4 rs7178270 GG genotype was associated with high risk in drinkers and AN chewers. CHRNB4 rs7178270 and AN chewing have an interaction effect on lung cancer in Hainan. Conclusions This study is the first to elucidate the hidden impacts of AN on lung cancer and provides a key evidence regarding the interactive effects of AN and cigarettes with SNPs in nAChRs genes on lung cancer. Graphical Abstract |
| format | Article |
| id | doaj-art-dd0c258ef18841b99677b66e81270e55 |
| institution | DOAJ |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-dd0c258ef18841b99677b66e81270e552025-08-20T03:06:51ZengBMCBMC Cancer1471-24072025-04-0125111710.1186/s12885-025-14020-3Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control studyYixuan Li0Jing Zhou1Lirong Liu2Chaoyong Zhu3Ziyue Luo4Na Li5Pengfei Lyu6Jing Zhang7Tian Xie8Yipeng Ding9Sha Xiao10School of Public Health, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Heinz Mehlhorn Academician Workstation, Hainan Medical UniversitySchool of Public Health, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Heinz Mehlhorn Academician Workstation, Hainan Medical UniversityDepartment of Respiratory and Critical Care Medicine, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityMedical Examination Center of Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversitySchool of Public Health, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Heinz Mehlhorn Academician Workstation, Hainan Medical UniversitySchool of Public Health, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Heinz Mehlhorn Academician Workstation, Hainan Medical UniversityDepartment of Breast Surgery, The First Affiliated Hospital of Hainan Medical UniversitySchool of Public Health, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Heinz Mehlhorn Academician Workstation, Hainan Medical UniversityDepartment of Respiratory and Critical Care Medicine, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversityDepartment of Respiratory and Critical Care Medicine, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical UniversitySchool of Public Health, Key Laboratory of Tropical Translational Medicine of Ministry of Education, Heinz Mehlhorn Academician Workstation, Hainan Medical UniversityAbstract Background Areca nut (AN) was classified as a carcinogen by the International Agency for Research on Cancer (IARC) of the WHO in 2003. AN has the same carcinogenic components as cigarettes, such as benzo[a]pyrene (B[a]P) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK), but its effects on interactions with genetic factors related to lung cancer have rarely been investigated. Methods Here, a propensity score-matched case‒control study was conducted in Hainan, which included 445 patients with lung cancer and 445 cancer-free controls. Then, the associations between single-nucleotide polymorphisms (SNPs) in the CHRNA5-CHRNA3-CHRNB4 gene cluster and their interaction effects with AN chewing and smoking on lung cancer were analyzed. In addition, we explored the associations among AN, cigarettes, and genes related to lung cancer using the Comparative Toxicogenomics Database (CTD). Results The results indicate that the CHRNA3 rs938682 (A > G) GG genotype (OR = 0.669, 95% CI = 0.454 ~ 0.984, P = 0.042) can decrease the risk of lung cancer. The CHRNB4 rs7178270 (C > G) GG genotype (OR = 1.729, 95% CI = 1.168 ~ 2.571, P = 0.006) can increase the risk of lung cancer. The CHRNA5 rs17486278 CC genotype was associated with a high risk in males, smokers, and drinkers. The CHRNA3 rs938682 GG genotype was associated with a low risk in AN chewers. The CHRNB4 rs7178270 GG genotype was associated with high risk in drinkers and AN chewers. CHRNB4 rs7178270 and AN chewing have an interaction effect on lung cancer in Hainan. Conclusions This study is the first to elucidate the hidden impacts of AN on lung cancer and provides a key evidence regarding the interactive effects of AN and cigarettes with SNPs in nAChRs genes on lung cancer. Graphical Abstracthttps://doi.org/10.1186/s12885-025-14020-3Areca nutCigarettenAChRsSNPLung cancer |
| spellingShingle | Yixuan Li Jing Zhou Lirong Liu Chaoyong Zhu Ziyue Luo Na Li Pengfei Lyu Jing Zhang Tian Xie Yipeng Ding Sha Xiao Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study BMC Cancer Areca nut Cigarette nAChRs SNP Lung cancer |
| title | Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study |
| title_full | Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study |
| title_fullStr | Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study |
| title_full_unstemmed | Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study |
| title_short | Association of SNPs in nAChRs genes, areca nut chewing and smoking, and their interaction with lung cancer in Hainan, China: a case control study |
| title_sort | association of snps in nachrs genes areca nut chewing and smoking and their interaction with lung cancer in hainan china a case control study |
| topic | Areca nut Cigarette nAChRs SNP Lung cancer |
| url | https://doi.org/10.1186/s12885-025-14020-3 |
| work_keys_str_mv | AT yixuanli associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT jingzhou associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT lirongliu associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT chaoyongzhu associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT ziyueluo associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT nali associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT pengfeilyu associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT jingzhang associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT tianxie associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT yipengding associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy AT shaxiao associationofsnpsinnachrsgenesarecanutchewingandsmokingandtheirinteractionwithlungcancerinhainanchinaacasecontrolstudy |