Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt
Introduction: Hepatitis C is a liver disease caused by the hepatitis C virus (HCV). It can cause both acute and chronic hepatitis infection. Based on secretion of required cytokines upon infection, HCV can improve its own RNA and successfully complete the replication cycle. Importantly, single nucle...
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The Journal of Infection in Developing Countries
2017-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/8127 |
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| author | Hany Khalil Mohamed Arfa Samir El-Masrey Sherif M EL-Sherbini Amal A Abd-Elaziz |
| author_facet | Hany Khalil Mohamed Arfa Samir El-Masrey Sherif M EL-Sherbini Amal A Abd-Elaziz |
| author_sort | Hany Khalil |
| collection | DOAJ |
| description | Introduction: Hepatitis C is a liver disease caused by the hepatitis C virus (HCV). It can cause both acute and chronic hepatitis infection. Based on secretion of required cytokines upon infection, HCV can improve its own RNA and successfully complete the replication cycle. Importantly, single nucleotide polymorphisms (SNPs) are the most common type of genetic variation and have been found to play a critical role in modulation of cellular cytokine production and interaction.
Methodology: A total of 100 blood samples were obtained from HCV patients, and 120 samples were obtained from healthy individuals who served as controls. SNPs of interleukin-10/592 (IL-10/592) and IL-4/589 were investigated for possible connection with HCV infection. Relative expression of IL-4, IL-6, and IL-10 were detected using real-time polymerase chain reaction and enzyme-linked immunosorbent assay
Results: The polymorphisms of IL-10 revealed a high rate of mutant genotype CC within the location IL-10/592 in HCV patients and controls, which resulted in low secretion of IL-10. Interestingly, the findings here demonstrate a positive association between HCV load of viremia and the mutant genotype IL-4-589/TT accompanied with low expression IL-4 in comparison with IL-6 expression.
Conclusions: These data suggest that the expression of IL-4 is inversely proportional to HCV load of viremia, and this connection is due to the high level of mutant genotype IL-4-589/TT in infected patients located in gene promoter and inhibits gene expression.
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| format | Article |
| id | doaj-art-dd098dfb06f14c60b2f97e4f54333df1 |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2017-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-dd098dfb06f14c60b2f97e4f54333df12025-08-20T02:57:21ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802017-03-01110310.3855/jidc.8127Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in EgyptHany Khalil0Mohamed Arfa1Samir El-Masrey2Sherif M EL-Sherbini3Amal A Abd-Elaziz4Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptGenetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptGenetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptGenetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptGenetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, EgyptIntroduction: Hepatitis C is a liver disease caused by the hepatitis C virus (HCV). It can cause both acute and chronic hepatitis infection. Based on secretion of required cytokines upon infection, HCV can improve its own RNA and successfully complete the replication cycle. Importantly, single nucleotide polymorphisms (SNPs) are the most common type of genetic variation and have been found to play a critical role in modulation of cellular cytokine production and interaction. Methodology: A total of 100 blood samples were obtained from HCV patients, and 120 samples were obtained from healthy individuals who served as controls. SNPs of interleukin-10/592 (IL-10/592) and IL-4/589 were investigated for possible connection with HCV infection. Relative expression of IL-4, IL-6, and IL-10 were detected using real-time polymerase chain reaction and enzyme-linked immunosorbent assay Results: The polymorphisms of IL-10 revealed a high rate of mutant genotype CC within the location IL-10/592 in HCV patients and controls, which resulted in low secretion of IL-10. Interestingly, the findings here demonstrate a positive association between HCV load of viremia and the mutant genotype IL-4-589/TT accompanied with low expression IL-4 in comparison with IL-6 expression. Conclusions: These data suggest that the expression of IL-4 is inversely proportional to HCV load of viremia, and this connection is due to the high level of mutant genotype IL-4-589/TT in infected patients located in gene promoter and inhibits gene expression. https://jidc.org/index.php/journal/article/view/8127single nucleotide polymorphismSNPinterleukinshepatitis C virus |
| spellingShingle | Hany Khalil Mohamed Arfa Samir El-Masrey Sherif M EL-Sherbini Amal A Abd-Elaziz Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt Journal of Infection in Developing Countries single nucleotide polymorphism SNP interleukins hepatitis C virus |
| title | Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt |
| title_full | Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt |
| title_fullStr | Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt |
| title_full_unstemmed | Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt |
| title_short | Single nucleotide polymorphisms of interleukins associated with hepatitis C virus infection in Egypt |
| title_sort | single nucleotide polymorphisms of interleukins associated with hepatitis c virus infection in egypt |
| topic | single nucleotide polymorphism SNP interleukins hepatitis C virus |
| url | https://jidc.org/index.php/journal/article/view/8127 |
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