DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress in <i>Arabidopsis thaliana</i>

DRB1 and DRB2 Are Required for an Appropriate miRNA-Mediated Molecular Response to Salt Stress in <i>Arabidopsis thaliana</i>

In plants, microRNAs (miRNAs) and their target genes have been demonstrated to form an essential component of the molecular response to salt stress. In <i>Arabidopsis thaliana</i> (<i>Arabidopsis</i>), DOUBLE-STRANDED RNA BINDING1 (DRB1) and DRB2 are required to produce speci...

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Bibliographic Details
Main Authors: Joseph L. Pegler, Jackson M. J. Oultram, Christopher P. L. Grof, Andrew L. Eamens
Format: Article
Language:English
Published: MDPI AG 2025-03-01
Series:Plants
Subjects:
<i>Arabidopsis thaliana</i> (<i>Arabidopsis</i>)
salt stress
microRNA (miRNA)
gene expression regulation
DOUBLE-STRANDED RNA BINDING (DRB) protein
DRB1
Online Access:https://www.mdpi.com/2223-7747/14/6/924
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Summary:In plants, microRNAs (miRNAs) and their target genes have been demonstrated to form an essential component of the molecular response to salt stress. In <i>Arabidopsis thaliana</i> (<i>Arabidopsis</i>), DOUBLE-STRANDED RNA BINDING1 (DRB1) and DRB2 are required to produce specific miRNA populations throughout normal development and in response to abiotic stress. The phenotypic and physiological assessment of 15-day-old wild-type <i>Arabidopsis</i> seedlings, and of the <i>drb1</i> and <i>drb2</i> mutants following a 7-day period of salt stress, revealed the <i>drb2</i> mutant to be more sensitive to salt stress than the <i>drb1</i> mutant. However, the assessment of miRNA abundance and miRNA target gene expression showed that the ability of both <i>drb</i> mutants to mount an appropriate miRNA-mediated molecular response to salt stress is defective. Furthermore, molecular profiling also showed that DRB1 and DRB2 are both required for miRNA production during salt stress, and that both a target transcript cleavage mode and a translational repression mode of RNA silencing are required to appropriately regulate miRNA target gene expression as part of the molecular response of <i>Arabidopsis</i> to salt stress. Taken together, the phenotypic, physiological, and molecular analyses performed here clearly show that all components of the miRNA pathway must be fully functional for <i>Arabidopsis</i> to mount an appropriate miRNA-mediated molecular response to salt stress.
ISSN:2223-7747

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