Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors
Abstract Celosia argentea is a plant known for producing bioactive compounds, including betalains, which possess various biological and pharmaceutical properties. This study aimed to investigate the effect of biotic and abiotic elicitors on betalains production and their antioxidant activity in cell...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-83096-x |
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| author | Kanchanok Mueangnak Haruthairat Kitwetcharoen Sudarat Thanonkeo Preekamol Klanrit Jirawan Apiraksakorn Poramaporn Klanrit Poramate Klanrit Pornthap Thanonkeo |
| author_facet | Kanchanok Mueangnak Haruthairat Kitwetcharoen Sudarat Thanonkeo Preekamol Klanrit Jirawan Apiraksakorn Poramaporn Klanrit Poramate Klanrit Pornthap Thanonkeo |
| author_sort | Kanchanok Mueangnak |
| collection | DOAJ |
| description | Abstract Celosia argentea is a plant known for producing bioactive compounds, including betalains, which possess various biological and pharmaceutical properties. This study aimed to investigate the effect of biotic and abiotic elicitors on betalains production and their antioxidant activity in cell suspension cultures of C. argentea. Various concentrations of chitosan, yeast extract, salicylic acid, methyl jasmonate, copper sulfate (CuSO4), and cobalt chloride (CoCl2) were evaluated. The results revealed that chitosan, salicylic acid, methyl jasmonate, and CuSO4 significantly improved betalains production in the cell suspension cultures. Among these elicitors, chitosan at 5.0 mg/L and CuSO4 at 6.4 µM were the most effective in enhancing betalains production, yielding the highest concentrations of 4.65 and 4.99 mg/g dry weight, respectively. Notably, the betalains derived from the elicitor-treated cultures exhibited greater antioxidant activity compared to the control. These findings suggest that chitosan and CuSO4 are promising elicitors for sustainable in vitro production of betalains from C. argentea cell suspension cultures on a commercial scale, owing to their ability to enhance betalains production and antioxidant activity. |
| format | Article |
| id | doaj-art-dcebbfe750894ac18ccf5e59054b5a31 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-dcebbfe750894ac18ccf5e59054b5a312025-01-05T12:15:22ZengNature PortfolioScientific Reports2045-23222025-01-0115111710.1038/s41598-024-83096-xEnhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitorsKanchanok Mueangnak0Haruthairat Kitwetcharoen1Sudarat Thanonkeo2Preekamol Klanrit3Jirawan Apiraksakorn4Poramaporn Klanrit5Poramate Klanrit6Pornthap Thanonkeo7Department of Biotechnology, Faculty of Technology, Khon Kaen UniversityWalai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham UniversityWalai Rukhavej Botanical Research Institute (WRBRI), Mahasarakham UniversityDepartment of Biotechnology, Faculty of Technology, Khon Kaen UniversityDepartment of Biotechnology, Faculty of Technology, Khon Kaen UniversityResearch Group of Chronic Inflammatory Oral Diseases and Systemic Diseases Associated with Oral Health, Department of Oral Biomedical Sciences, Faculty of Dentistry, Khon Kaen UniversityDepartment of System Biosciences and Computational Medicine, Faculty of Medicine, Khon Kaen UniversityDepartment of Biotechnology, Faculty of Technology, Khon Kaen UniversityAbstract Celosia argentea is a plant known for producing bioactive compounds, including betalains, which possess various biological and pharmaceutical properties. This study aimed to investigate the effect of biotic and abiotic elicitors on betalains production and their antioxidant activity in cell suspension cultures of C. argentea. Various concentrations of chitosan, yeast extract, salicylic acid, methyl jasmonate, copper sulfate (CuSO4), and cobalt chloride (CoCl2) were evaluated. The results revealed that chitosan, salicylic acid, methyl jasmonate, and CuSO4 significantly improved betalains production in the cell suspension cultures. Among these elicitors, chitosan at 5.0 mg/L and CuSO4 at 6.4 µM were the most effective in enhancing betalains production, yielding the highest concentrations of 4.65 and 4.99 mg/g dry weight, respectively. Notably, the betalains derived from the elicitor-treated cultures exhibited greater antioxidant activity compared to the control. These findings suggest that chitosan and CuSO4 are promising elicitors for sustainable in vitro production of betalains from C. argentea cell suspension cultures on a commercial scale, owing to their ability to enhance betalains production and antioxidant activity.https://doi.org/10.1038/s41598-024-83096-xAntioxidant activityBetalainsCell suspension cultureCelosia argenteaElicitationPlant secondary metabolites |
| spellingShingle | Kanchanok Mueangnak Haruthairat Kitwetcharoen Sudarat Thanonkeo Preekamol Klanrit Jirawan Apiraksakorn Poramaporn Klanrit Poramate Klanrit Pornthap Thanonkeo Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors Scientific Reports Antioxidant activity Betalains Cell suspension culture Celosia argentea Elicitation Plant secondary metabolites |
| title | Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors |
| title_full | Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors |
| title_fullStr | Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors |
| title_full_unstemmed | Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors |
| title_short | Enhancing betalains production and antioxidant activity in Celosia argentea cell suspension cultures using biotic and abiotic elicitors |
| title_sort | enhancing betalains production and antioxidant activity in celosia argentea cell suspension cultures using biotic and abiotic elicitors |
| topic | Antioxidant activity Betalains Cell suspension culture Celosia argentea Elicitation Plant secondary metabolites |
| url | https://doi.org/10.1038/s41598-024-83096-x |
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