The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin

<i>Background and Objectives</i>: Obesity has become one of the most significant health problems nowadays, with its prevalence rapidly increasing. Approaches such as diet and exercise play an important role in the treatment of obesity. This study aimed to investigate the responses of uri...

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Main Authors: Taner Akbulut, Vedat Cinar, Emsal Cagla Avcu, Yavuz Yasul, İsa Aydemir, Tuncay Kuloglu, Gokhan Artas, Suleyman Aydin
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Language:English
Published: MDPI AG 2025-02-01
Series:Medicina
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Online Access:https://www.mdpi.com/1648-9144/61/3/399
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author Taner Akbulut
Vedat Cinar
Emsal Cagla Avcu
Yavuz Yasul
İsa Aydemir
Tuncay Kuloglu
Gokhan Artas
Suleyman Aydin
author_facet Taner Akbulut
Vedat Cinar
Emsal Cagla Avcu
Yavuz Yasul
İsa Aydemir
Tuncay Kuloglu
Gokhan Artas
Suleyman Aydin
author_sort Taner Akbulut
collection DOAJ
description <i>Background and Objectives</i>: Obesity has become one of the most significant health problems nowadays, with its prevalence rapidly increasing. Approaches such as diet and exercise play an important role in the treatment of obesity. This study aimed to investigate the responses of uric acid, irisin, adiponutrin, adropin, and copeptin levels to exercise and metformin intervention in obesity. <i>Materials and Methods</i>: Thirty-six male Sprague–Dawley rats were randomly divided into seven groups: healthy control (HC), sham (S), obese control (OC), metformin (M), exercise (E), metformin + exercise (ME), and decapitation (D). After obesity was induced through a 12-week high-fat diet, obese rats underwent a 4-week aerobic exercise and metformin intervention. <i>Results</i>: Uric acid, irisin, adiponutrin, adropin, and copeptin levels were determined using an ELISA method. Copeptin levels significantly decreased in the ME group (<i>p</i> < 0.001). Irisin levels significantly increased in the E and ME groups (<i>p</i> < 0.001). The most notable increases in adropin levels occurred in the E and ME groups (<i>p</i> < 0.001). Uric acid levels were highest in the OC group but significantly lower in the E and M groups (<i>p</i> < 0.001). Adiponutrin levels did not change in response to exercise or metformin intervention in obesity (<i>p</i> > 0.05). <i>Conclusions</i>: These findings suggest that exercise and metformin intervention may play an effective role in obesity management.
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spelling doaj-art-dce87b60b64a447c8bbc57dcd4b19f7c2025-08-20T02:42:22ZengMDPI AGMedicina1010-660X1648-91442025-02-0161339910.3390/medicina61030399The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and CopeptinTaner Akbulut0Vedat Cinar1Emsal Cagla Avcu2Yavuz Yasul3İsa Aydemir4Tuncay Kuloglu5Gokhan Artas6Suleyman Aydin7Department of Coaching Education, Faculty of Sports Sciences, Firat University, Elazig 23119, TurkeyDepartment of Physical Education and Sports Teaching, Faculty of Sport Sciences, Firat University, Elazig 23119, TurkeyDepartment of Coaching Education, Faculty of Sport Sciences, Sivas Cumhuriyet University, Sivas 58140, TurkeyProperty Protection and Security Division, Bafra Vocational School, Ondokuz Mayıs University, Samsun 55400, TurkeyFaculty of Education, Physical Education and Sports, Hakkari University, Hakkari 30000, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Firat University, Elazig 23119, TurkeyDepartment of Medical Pathology, Faculty of Medicine, Firat University, Elazig 23119, TurkeyDepartment of Biochemistry, Faculty of Medicine, Firat University, Elazig 23119, Turkey<i>Background and Objectives</i>: Obesity has become one of the most significant health problems nowadays, with its prevalence rapidly increasing. Approaches such as diet and exercise play an important role in the treatment of obesity. This study aimed to investigate the responses of uric acid, irisin, adiponutrin, adropin, and copeptin levels to exercise and metformin intervention in obesity. <i>Materials and Methods</i>: Thirty-six male Sprague–Dawley rats were randomly divided into seven groups: healthy control (HC), sham (S), obese control (OC), metformin (M), exercise (E), metformin + exercise (ME), and decapitation (D). After obesity was induced through a 12-week high-fat diet, obese rats underwent a 4-week aerobic exercise and metformin intervention. <i>Results</i>: Uric acid, irisin, adiponutrin, adropin, and copeptin levels were determined using an ELISA method. Copeptin levels significantly decreased in the ME group (<i>p</i> < 0.001). Irisin levels significantly increased in the E and ME groups (<i>p</i> < 0.001). The most notable increases in adropin levels occurred in the E and ME groups (<i>p</i> < 0.001). Uric acid levels were highest in the OC group but significantly lower in the E and M groups (<i>p</i> < 0.001). Adiponutrin levels did not change in response to exercise or metformin intervention in obesity (<i>p</i> > 0.05). <i>Conclusions</i>: These findings suggest that exercise and metformin intervention may play an effective role in obesity management.https://www.mdpi.com/1648-9144/61/3/399exercisemetforminobesityadipokinesmyokines
spellingShingle Taner Akbulut
Vedat Cinar
Emsal Cagla Avcu
Yavuz Yasul
İsa Aydemir
Tuncay Kuloglu
Gokhan Artas
Suleyman Aydin
The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin
Medicina
exercise
metformin
obesity
adipokines
myokines
title The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin
title_full The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin
title_fullStr The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin
title_full_unstemmed The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin
title_short The Regulatory Effects of Exercise and Metformin on Biomarkers in Obesity: A Focus on Uric Acid, Irisin, Adiponutrin, Adropin, and Copeptin
title_sort regulatory effects of exercise and metformin on biomarkers in obesity a focus on uric acid irisin adiponutrin adropin and copeptin
topic exercise
metformin
obesity
adipokines
myokines
url https://www.mdpi.com/1648-9144/61/3/399
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