Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm
Thermophilic bacteria living in extreme areas with high temperatures are capable of producing secondary metabolites, such as antimicrobial peptides (AMPs). AMPs are stable at high temperatures and show good antibacterial activity. Therefore, this study aimed to identify thermophilic bacteria from th...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/ijod/4285984 |
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| author | Emma Rachmawati Shinta Asarina Gabriel Bagus Kennardi Akeyla Tabina Tawangalun Candra Arumimaniyah Kartika Indah Sari Hening Tjaturina Pramesti Ratu Safitri Ani Melani Maskoen |
| author_facet | Emma Rachmawati Shinta Asarina Gabriel Bagus Kennardi Akeyla Tabina Tawangalun Candra Arumimaniyah Kartika Indah Sari Hening Tjaturina Pramesti Ratu Safitri Ani Melani Maskoen |
| author_sort | Emma Rachmawati |
| collection | DOAJ |
| description | Thermophilic bacteria living in extreme areas with high temperatures are capable of producing secondary metabolites, such as antimicrobial peptides (AMPs). AMPs are stable at high temperatures and show good antibacterial activity. Therefore, this study aimed to identify thermophilic bacteria from the crater of Mount Tangkuban Perahu around West Java and assess antibacterial effectiveness of AMPs against Streptococcus mutans, which contribute to oral biofilm formation. The isolate obtained was identified using 16S ribosomal ribonucleic acid (rRNA) gene sequencing, and the supernatant of the isolate was tested against S. mutans American Type Culture Collection (ATCC) 25175 using the disc assay method. To determine AMPs-coding genes, its genome was uploaded to antibiotic and secondary metabolite analysis shell (antiSMASH) 5.0.0 platform and biofilm inhibition was tested using the microtiter plate technique (with a 96-well bottom). Subsequently, the results were assessed using a microplate reader operating at 595 nm wavelength. The isolate was identified as Geobacillus subterraneus, with antibacterial activity against S. mutans, and produced an inhibition zone of 8.40 mm at an optimum pH of 8. The output of AMPs-coding gene showed that AMPs of the isolate were a member of the lanthipeptide class I, or bacteriocin-I group. AMPs of G. subterraneus suppressed the growth of S. mutans biofilm at a supernatant concentration of 5%, with the lowest optical density (OD) value of 0.061 and the highest percentage of biofilm growth inhibition at 28.24%. Based on the results, G. subterraneus derived from the crater of Mount Tangkuban Perahu showed potent antibacterial properties against S. mutans, making it a promising novel S. mutans anti-biofilm candidate. |
| format | Article |
| id | doaj-art-dcdcb09b42e740d0931847036edbf499 |
| institution | OA Journals |
| issn | 1687-8736 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-dcdcb09b42e740d0931847036edbf4992025-08-20T02:38:47ZengWileyInternational Journal of Dentistry1687-87362024-01-01202410.1155/ijod/4285984Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-BiofilmEmma Rachmawati0Shinta Asarina1Gabriel Bagus Kennardi2Akeyla Tabina Tawangalun3Candra Arumimaniyah4Kartika Indah Sari5Hening Tjaturina Pramesti6Ratu Safitri7Ani Melani Maskoen8Department of Oral BiologyMicrobiology Laboratory AssistantBiotechnology StudentDepartment of BiologyDepartment of BiologyDepartment of Oral BiologyDepartment of Oral BiologySenior Lecturer of the Biotechnology Study ProgramDepartment of Oral BiologyThermophilic bacteria living in extreme areas with high temperatures are capable of producing secondary metabolites, such as antimicrobial peptides (AMPs). AMPs are stable at high temperatures and show good antibacterial activity. Therefore, this study aimed to identify thermophilic bacteria from the crater of Mount Tangkuban Perahu around West Java and assess antibacterial effectiveness of AMPs against Streptococcus mutans, which contribute to oral biofilm formation. The isolate obtained was identified using 16S ribosomal ribonucleic acid (rRNA) gene sequencing, and the supernatant of the isolate was tested against S. mutans American Type Culture Collection (ATCC) 25175 using the disc assay method. To determine AMPs-coding genes, its genome was uploaded to antibiotic and secondary metabolite analysis shell (antiSMASH) 5.0.0 platform and biofilm inhibition was tested using the microtiter plate technique (with a 96-well bottom). Subsequently, the results were assessed using a microplate reader operating at 595 nm wavelength. The isolate was identified as Geobacillus subterraneus, with antibacterial activity against S. mutans, and produced an inhibition zone of 8.40 mm at an optimum pH of 8. The output of AMPs-coding gene showed that AMPs of the isolate were a member of the lanthipeptide class I, or bacteriocin-I group. AMPs of G. subterraneus suppressed the growth of S. mutans biofilm at a supernatant concentration of 5%, with the lowest optical density (OD) value of 0.061 and the highest percentage of biofilm growth inhibition at 28.24%. Based on the results, G. subterraneus derived from the crater of Mount Tangkuban Perahu showed potent antibacterial properties against S. mutans, making it a promising novel S. mutans anti-biofilm candidate.http://dx.doi.org/10.1155/ijod/4285984 |
| spellingShingle | Emma Rachmawati Shinta Asarina Gabriel Bagus Kennardi Akeyla Tabina Tawangalun Candra Arumimaniyah Kartika Indah Sari Hening Tjaturina Pramesti Ratu Safitri Ani Melani Maskoen Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm International Journal of Dentistry |
| title | Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm |
| title_full | Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm |
| title_fullStr | Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm |
| title_full_unstemmed | Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm |
| title_short | Isolation of Thermophilic Bacteria Geobacillus subterraneus From Mount Tangkuban Perahu and the Novelty as a Candidate for Streptococcus mutans Anti-Biofilm |
| title_sort | isolation of thermophilic bacteria geobacillus subterraneus from mount tangkuban perahu and the novelty as a candidate for streptococcus mutans anti biofilm |
| url | http://dx.doi.org/10.1155/ijod/4285984 |
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