Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity
<b>Obiective:</b> In the study, corn bran powder was used as raw material, and the cell wall was destroyed by ultrasonic vibration and enzymatic hydrolysis, so that corn bran polysaccharide could be dissolved more fully. <b>Methods:</b> The extracted amount of polysaccharide...
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The Editorial Office of Food and Machinery
2023-06-01
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| Series: | Shipin yu jixie |
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| Online Access: | http://www.ifoodmm.com/spyjxen/article/abstract/20230530 |
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| author | MA Li-yuan HUANG Xue-ying SHANG Er-kun GUO Li ZHANG Ya-na |
| author_facet | MA Li-yuan HUANG Xue-ying SHANG Er-kun GUO Li ZHANG Ya-na |
| author_sort | MA Li-yuan |
| collection | DOAJ |
| description | <b>Obiective:</b> In the study, corn bran powder was used as raw material, and the cell wall was destroyed by ultrasonic vibration and enzymatic hydrolysis, so that corn bran polysaccharide could be dissolved more fully. <b>Methods:</b> The extracted amount of polysaccharide was taken as the index, response surface test optimization was carried out on the basis of single factor test. And the antioxidant activity of corn bran polysaccharide was determined. <b>Results:</b> The optimal extraction process of corn bran polysaccharide was obtained as follows: mass ratio of cellulase to pectinase 2∶1, enzyme dosage was 2% of the quality of corn bran powder, enzymatic hydrolysis time 50 min, ultrasonic treatment 15 min, and solid-liquid ratio （<i>m</i><sub>corn bran powder</sub>∶<i>V</i><sub>distilled water</sub>） 1∶15 （g/mL）. At this time, the average amount of extracted corn bran polysaccharide was 23.36 μg/g. The extracted corn bran polysaccharide had a certain total reducing capacity, scavenging DPPH free radical and hydroxyl radical ability, and it increased with the increase of mass concentration, when the mass concentration of corn bran polysaccharide sample was 0.2~1.0 mg/mL. <b>Conclusion:</b> Ultrasound-assisted enzymatic method could extract more polysaccharides than single enzymatic method, and the extract could maintain efficient antioxidant capacity. |
| format | Article |
| id | doaj-art-dcdcaf2dba6b40f49fdd9906b5292e0e |
| institution | OA Journals |
| issn | 1003-5788 |
| language | English |
| publishDate | 2023-06-01 |
| publisher | The Editorial Office of Food and Machinery |
| record_format | Article |
| series | Shipin yu jixie |
| spelling | doaj-art-dcdcaf2dba6b40f49fdd9906b5292e0e2025-08-20T02:23:32ZengThe Editorial Office of Food and MachineryShipin yu jixie1003-57882023-06-01395186192,20210.13652/j.spjx.1003.5788.2022.80781Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activityMA Li-yuan0HUANG Xue-ying1SHANG Er-kun2GUO Li3ZHANG Ya-na4 College of Food and Pharmaceutical Engineering, Suihua University, Suihua, Heilongjiang 152061 , China College of Food and Pharmaceutical Engineering, Suihua University, Suihua, Heilongjiang 152061 , China Suihua Inspection and Testing Center, Suihua, Heilongjiang 152000 , China College of Food and Pharmaceutical Engineering, Suihua University, Suihua, Heilongjiang 152061 , China College of Food and Pharmaceutical Engineering, Suihua University, Suihua, Heilongjiang 152061 , China <b>Obiective:</b> In the study, corn bran powder was used as raw material, and the cell wall was destroyed by ultrasonic vibration and enzymatic hydrolysis, so that corn bran polysaccharide could be dissolved more fully. <b>Methods:</b> The extracted amount of polysaccharide was taken as the index, response surface test optimization was carried out on the basis of single factor test. And the antioxidant activity of corn bran polysaccharide was determined. <b>Results:</b> The optimal extraction process of corn bran polysaccharide was obtained as follows: mass ratio of cellulase to pectinase 2∶1, enzyme dosage was 2% of the quality of corn bran powder, enzymatic hydrolysis time 50 min, ultrasonic treatment 15 min, and solid-liquid ratio （<i>m</i><sub>corn bran powder</sub>∶<i>V</i><sub>distilled water</sub>） 1∶15 （g/mL）. At this time, the average amount of extracted corn bran polysaccharide was 23.36 μg/g. The extracted corn bran polysaccharide had a certain total reducing capacity, scavenging DPPH free radical and hydroxyl radical ability, and it increased with the increase of mass concentration, when the mass concentration of corn bran polysaccharide sample was 0.2~1.0 mg/mL. <b>Conclusion:</b> Ultrasound-assisted enzymatic method could extract more polysaccharides than single enzymatic method, and the extract could maintain efficient antioxidant capacity.http://www.ifoodmm.com/spyjxen/article/abstract/20230530 corn bran polysaccharide ultrasound assisted enzymatic method antioxidant activity |
| spellingShingle | MA Li-yuan HUANG Xue-ying SHANG Er-kun GUO Li ZHANG Ya-na Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity Shipin yu jixie corn bran polysaccharide ultrasound assisted enzymatic method antioxidant activity |
| title | Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity |
| title_full | Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity |
| title_fullStr | Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity |
| title_full_unstemmed | Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity |
| title_short | Ultrasound-assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity |
| title_sort | ultrasound assisted enzymatic extraction of polysaccharides from corn bran and determination of their antioxidant activity |
| topic | corn bran polysaccharide ultrasound assisted enzymatic method antioxidant activity |
| url | http://www.ifoodmm.com/spyjxen/article/abstract/20230530 |
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