Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants

Multicellular organisms exhibit inherent cellular heterogeneity that cannot be captured by traditional high-throughput sequencing techniques, resulting in the unique cellular characteristics of individual cells being neglected. Single-cell transcriptome sequencing (scRNA-seq) technology can be used...

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Main Authors: Jianwen Bian, Zelong Zhuang, Xiangzhuo Ji, Rui Tang, Jiawei Li, Jiangtao Chen, Zhiming Li, Yunling Peng
Format: Article
Language:English
Published: MDPI AG 2024-10-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/14/11/2530
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author Jianwen Bian
Zelong Zhuang
Xiangzhuo Ji
Rui Tang
Jiawei Li
Jiangtao Chen
Zhiming Li
Yunling Peng
author_facet Jianwen Bian
Zelong Zhuang
Xiangzhuo Ji
Rui Tang
Jiawei Li
Jiangtao Chen
Zhiming Li
Yunling Peng
author_sort Jianwen Bian
collection DOAJ
description Multicellular organisms exhibit inherent cellular heterogeneity that cannot be captured by traditional high-throughput sequencing techniques, resulting in the unique cellular characteristics of individual cells being neglected. Single-cell transcriptome sequencing (scRNA-seq) technology can be used to determine the gene expression levels of each individual cell, facilitating the study of intercellular expression heterogeneity. This review provides a comprehensive overview of the development and applications of scRNA-seq technology in plant research. We highlight the significance of integrating single-cell multi-omics approaches to achieve a holistic understanding of plant systems. Additionally, we discuss the current challenges and future research directions for scRNA-seq technology in plant studies, aiming to offer valuable insights for its application across various plant species.
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id doaj-art-dcdc25a0fcc5480bb86fa6f5d60a86ab
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issn 2073-4395
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publishDate 2024-10-01
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series Agronomy
spelling doaj-art-dcdc25a0fcc5480bb86fa6f5d60a86ab2025-08-20T02:08:11ZengMDPI AGAgronomy2073-43952024-10-011411253010.3390/agronomy14112530Research Progress of Single-Cell Transcriptome Sequencing Technology in PlantsJianwen Bian0Zelong Zhuang1Xiangzhuo Ji2Rui Tang3Jiawei Li4Jiangtao Chen5Zhiming Li6Yunling Peng7College of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaMulticellular organisms exhibit inherent cellular heterogeneity that cannot be captured by traditional high-throughput sequencing techniques, resulting in the unique cellular characteristics of individual cells being neglected. Single-cell transcriptome sequencing (scRNA-seq) technology can be used to determine the gene expression levels of each individual cell, facilitating the study of intercellular expression heterogeneity. This review provides a comprehensive overview of the development and applications of scRNA-seq technology in plant research. We highlight the significance of integrating single-cell multi-omics approaches to achieve a holistic understanding of plant systems. Additionally, we discuss the current challenges and future research directions for scRNA-seq technology in plant studies, aiming to offer valuable insights for its application across various plant species.https://www.mdpi.com/2073-4395/14/11/2530plantsingle-cell transcriptomecell atlasdevelopmental trajectorymulti-omics
spellingShingle Jianwen Bian
Zelong Zhuang
Xiangzhuo Ji
Rui Tang
Jiawei Li
Jiangtao Chen
Zhiming Li
Yunling Peng
Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants
Agronomy
plant
single-cell transcriptome
cell atlas
developmental trajectory
multi-omics
title Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants
title_full Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants
title_fullStr Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants
title_full_unstemmed Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants
title_short Research Progress of Single-Cell Transcriptome Sequencing Technology in Plants
title_sort research progress of single cell transcriptome sequencing technology in plants
topic plant
single-cell transcriptome
cell atlas
developmental trajectory
multi-omics
url https://www.mdpi.com/2073-4395/14/11/2530
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AT jiangtaochen researchprogressofsinglecelltranscriptomesequencingtechnologyinplants
AT zhimingli researchprogressofsinglecelltranscriptomesequencingtechnologyinplants
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