Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing
The bioconversion of xylose into xylitol offers a sustainable approach to upcycle pentoses in lignocellulosic hydrolysates by leveraging the metabolic capabilities of ascomycetous yeasts. Scalable solutions, such as immobilized-cell systems, facilitate industrial bioprocessing by enabling easy bioma...
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| Language: | English |
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AIDIC Servizi S.r.l.
2025-07-01
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| Series: | Chemical Engineering Transactions |
| Online Access: | https://www.cetjournal.it/index.php/cet/article/view/15256 |
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| author | Federico Ferretti Stefano Raimondi Francesco Candeliere Alan Leonardi Maddalena Rossi Alberto Amaretti |
| author_facet | Federico Ferretti Stefano Raimondi Francesco Candeliere Alan Leonardi Maddalena Rossi Alberto Amaretti |
| author_sort | Federico Ferretti |
| collection | DOAJ |
| description | The bioconversion of xylose into xylitol offers a sustainable approach to upcycle pentoses in lignocellulosic hydrolysates by leveraging the metabolic capabilities of ascomycetous yeasts. Scalable solutions, such as immobilized-cell systems, facilitate industrial bioprocessing by enabling easy biomass recovery, reusability, and maintenance of high cell concentrations without compromising productivity. Pichia fermentans WC 1507 has demonstrated remarkable efficiency in converting xylose to xylitol. In this study, its cells were immobilized in beads composed of 0.8% (w/v) alginate and 10% (w/v) polyvinyl alcohol (PVA) crosslinked with calcium and boric acid. While immobilization initially reduce yeast viability, it was progressively restored during the first utilization cycles of the PVA-immobilized cells.
The PVA beads loaded with the cells were tested in four consecutive 7-day fermentation runs using a medium containing 120 g/L xylose. The experiments confirmed the excellent robustness of the PVA beads, that proved to be usable in stirred tank bioreactors for remarkably long periods. Across the four runs, fermentation efficiency improved, achieving stable xylitol yields of 60–63 g/L, with 54–56% conversion and a volumetric productivity of 450–470 mg/L/h under non-optimized conditions.
These results highlighted the potential of P. fermentans WC 1507 for the valorization of xylose-rich hydrolysate and validated PVA matrices as durable biomass carrier for use in stirred bioreactor systems. |
| format | Article |
| id | doaj-art-dcd7850b4e7045828458433f20a37008 |
| institution | Kabale University |
| issn | 2283-9216 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | AIDIC Servizi S.r.l. |
| record_format | Article |
| series | Chemical Engineering Transactions |
| spelling | doaj-art-dcd7850b4e7045828458433f20a370082025-08-20T03:34:49ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162025-07-01117Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol BioprocessingFederico FerrettiStefano RaimondiFrancesco CandeliereAlan LeonardiMaddalena RossiAlberto AmarettiThe bioconversion of xylose into xylitol offers a sustainable approach to upcycle pentoses in lignocellulosic hydrolysates by leveraging the metabolic capabilities of ascomycetous yeasts. Scalable solutions, such as immobilized-cell systems, facilitate industrial bioprocessing by enabling easy biomass recovery, reusability, and maintenance of high cell concentrations without compromising productivity. Pichia fermentans WC 1507 has demonstrated remarkable efficiency in converting xylose to xylitol. In this study, its cells were immobilized in beads composed of 0.8% (w/v) alginate and 10% (w/v) polyvinyl alcohol (PVA) crosslinked with calcium and boric acid. While immobilization initially reduce yeast viability, it was progressively restored during the first utilization cycles of the PVA-immobilized cells. The PVA beads loaded with the cells were tested in four consecutive 7-day fermentation runs using a medium containing 120 g/L xylose. The experiments confirmed the excellent robustness of the PVA beads, that proved to be usable in stirred tank bioreactors for remarkably long periods. Across the four runs, fermentation efficiency improved, achieving stable xylitol yields of 60–63 g/L, with 54–56% conversion and a volumetric productivity of 450–470 mg/L/h under non-optimized conditions. These results highlighted the potential of P. fermentans WC 1507 for the valorization of xylose-rich hydrolysate and validated PVA matrices as durable biomass carrier for use in stirred bioreactor systems.https://www.cetjournal.it/index.php/cet/article/view/15256 |
| spellingShingle | Federico Ferretti Stefano Raimondi Francesco Candeliere Alan Leonardi Maddalena Rossi Alberto Amaretti Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing Chemical Engineering Transactions |
| title | Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing |
| title_full | Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing |
| title_fullStr | Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing |
| title_full_unstemmed | Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing |
| title_short | Polyvinyl Alcohol Beads as a Robust and Reusable Carrier for Pichia fermentans WC1507 in Xylose-to-Xylitol Bioprocessing |
| title_sort | polyvinyl alcohol beads as a robust and reusable carrier for pichia fermentans wc1507 in xylose to xylitol bioprocessing |
| url | https://www.cetjournal.it/index.php/cet/article/view/15256 |
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