Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures

Abstract Micromagnetic stimulation (µMS) emerges as a complementary method for neuromodulation. Despite major advances in neural interface technology, there are limited options for neural inhibition. Here, a microchip‐based implantable micro‐coil device is presented to achieve high spatial precision...

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Main Authors: Kayeon Kim, Xiyuan Liu, Bingdong Chang, Guanghui Li, Gwendoline A. E. Anand, Su Genelioglu, Alexandra Katherine Isis Yonza, Andrew J Whalen, Rune W Berg, Shelley I Fried, Anpan Han, Changsi Cai
Format: Article
Language:English
Published: Wiley 2025-06-01
Series:Advanced Science
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Online Access:https://doi.org/10.1002/advs.202416771
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author Kayeon Kim
Xiyuan Liu
Bingdong Chang
Guanghui Li
Gwendoline A. E. Anand
Su Genelioglu
Alexandra Katherine Isis Yonza
Andrew J Whalen
Rune W Berg
Shelley I Fried
Anpan Han
Changsi Cai
author_facet Kayeon Kim
Xiyuan Liu
Bingdong Chang
Guanghui Li
Gwendoline A. E. Anand
Su Genelioglu
Alexandra Katherine Isis Yonza
Andrew J Whalen
Rune W Berg
Shelley I Fried
Anpan Han
Changsi Cai
author_sort Kayeon Kim
collection DOAJ
description Abstract Micromagnetic stimulation (µMS) emerges as a complementary method for neuromodulation. Despite major advances in neural interface technology, there are limited options for neural inhibition. Here, a microchip‐based implantable micro‐coil device is presented to achieve high spatial precision for cortical inhibition. Cortical in vivo two‐photon imaging of spontaneous neural activity showed µMS reversibly suppressed single cells, and as µMS magnitude is increased, the suppressed cell population increased from 14% to 41%. At the circuit level, the average suppressed area is 0.05 mm2, seven times smaller than the activated area induced by micro‐electrode stimulation (µES). It is discovered that neurons responded more strongly to µMS than to µES, which is exploited to effectively neutralize the neural excitation induced by concurrently delivered strong µES (80 µA). Moreover, µMS mitigates hyperactive neural firing caused by pharmacologically induced seizures, reducing seizure amplitude by 54%. These findings underscore the potential of µMS as a precise, effective, and versatile tool for localized neuromodulation with an effect of opposite polarity from µES. Complementing optogenetic and electrical stimulation for multi‐functional neural interfaces, µMS holds promise as a unique neuroscience research tool and as a potential therapeutic intervention method for precisely suppressing hyperactive brain circuits.
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spelling doaj-art-dcd66ed71f844763b2b47dc3c63c78a12025-08-20T03:44:51ZengWileyAdvanced Science2198-38442025-06-011222n/an/a10.1002/advs.202416771Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing SeizuresKayeon Kim0Xiyuan Liu1Bingdong Chang2Guanghui Li3Gwendoline A. E. Anand4Su Genelioglu5Alexandra Katherine Isis Yonza6Andrew J Whalen7Rune W Berg8Shelley I Fried9Anpan Han10Changsi Cai11Department of Neuroscience Faculty of Health and Medical Science University of Copenhagen Copenhagen DK‐2200 DenmarkDepartment of Neuroscience Faculty of Health and Medical Science University of Copenhagen Copenhagen DK‐2200 DenmarkDepartment of Civil and Mechanical Engineering Technical University of Denmark Lyngby 2800 DenmarkDepartment of Neuroscience Faculty of Health and Medical Science University of Copenhagen Copenhagen DK‐2200 DenmarkDepartment of Civil and Mechanical Engineering Technical University of Denmark Lyngby 2800 DenmarkDepartment of Civil and Mechanical Engineering Technical University of Denmark Lyngby 2800 DenmarkDepartment of Neuroscience Faculty of Health and Medical Science University of Copenhagen Copenhagen DK‐2200 DenmarkDepartment of Neurosurgery Yale School of Medicine New Haven CT 06510 USADepartment of Neuroscience Faculty of Health and Medical Science University of Copenhagen Copenhagen DK‐2200 DenmarkBoston VA Healthcare System Boston MA 02130 USADepartment of Civil and Mechanical Engineering Technical University of Denmark Lyngby 2800 DenmarkDepartment of Neuroscience Faculty of Health and Medical Science University of Copenhagen Copenhagen DK‐2200 DenmarkAbstract Micromagnetic stimulation (µMS) emerges as a complementary method for neuromodulation. Despite major advances in neural interface technology, there are limited options for neural inhibition. Here, a microchip‐based implantable micro‐coil device is presented to achieve high spatial precision for cortical inhibition. Cortical in vivo two‐photon imaging of spontaneous neural activity showed µMS reversibly suppressed single cells, and as µMS magnitude is increased, the suppressed cell population increased from 14% to 41%. At the circuit level, the average suppressed area is 0.05 mm2, seven times smaller than the activated area induced by micro‐electrode stimulation (µES). It is discovered that neurons responded more strongly to µMS than to µES, which is exploited to effectively neutralize the neural excitation induced by concurrently delivered strong µES (80 µA). Moreover, µMS mitigates hyperactive neural firing caused by pharmacologically induced seizures, reducing seizure amplitude by 54%. These findings underscore the potential of µMS as a precise, effective, and versatile tool for localized neuromodulation with an effect of opposite polarity from µES. Complementing optogenetic and electrical stimulation for multi‐functional neural interfaces, µMS holds promise as a unique neuroscience research tool and as a potential therapeutic intervention method for precisely suppressing hyperactive brain circuits.https://doi.org/10.1002/advs.202416771MEMs micro‐coilneural inhibitionneural interfacesingle‐cell studytwo‐photon imaging
spellingShingle Kayeon Kim
Xiyuan Liu
Bingdong Chang
Guanghui Li
Gwendoline A. E. Anand
Su Genelioglu
Alexandra Katherine Isis Yonza
Andrew J Whalen
Rune W Berg
Shelley I Fried
Anpan Han
Changsi Cai
Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures
Advanced Science
MEMs micro‐coil
neural inhibition
neural interface
single‐cell study
two‐photon imaging
title Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures
title_full Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures
title_fullStr Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures
title_full_unstemmed Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures
title_short Micro‐Coil Neuromodulation at Single‐Cell and Circuit Levels for Inhibiting Natural Neuroactivity, Neutralizing Electric Neural Excitation, and Suppressing Seizures
title_sort micro coil neuromodulation at single cell and circuit levels for inhibiting natural neuroactivity neutralizing electric neural excitation and suppressing seizures
topic MEMs micro‐coil
neural inhibition
neural interface
single‐cell study
two‐photon imaging
url https://doi.org/10.1002/advs.202416771
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