Evaluation of polyherbal synergy against diabetes: in-vitro analysis
Aims To evaluate the anti-diabetic properties of a polyherbal formulation consisting of Curcuma longa, Emblica officinalis, and Trigonella foenum-graecum through in-vitro assays, and investigate its synergistic effects on glucose metabolism, lipid metabolism, and insulin function.Materials and metho...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2025-12-01
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| Series: | Future Science OA |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/20565623.2025.2468128 |
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| author | Sridevi N. Thirumal M. |
| author_facet | Sridevi N. Thirumal M. |
| author_sort | Sridevi N. |
| collection | DOAJ |
| description | Aims To evaluate the anti-diabetic properties of a polyherbal formulation consisting of Curcuma longa, Emblica officinalis, and Trigonella foenum-graecum through in-vitro assays, and investigate its synergistic effects on glucose metabolism, lipid metabolism, and insulin function.Materials and methods The polyherbal extract was prepared using methanol, water, and methanol-water as solvents. In-vitro antioxidant (DPPH, Phosphomolybdenum) and anti-diabetic (α-amylase, α-glucosidase inhibition, yeast glucose uptake, and inhibitory glucose diffusion) assays were conducted. L6 rat skeletal muscle cells were used for MTT assay and glucose uptake.Results The methanol extract showed the highest antioxidant and anti-diabetic activities compared to aqueous and hydroalcoholic extracts. The IC50 values in DPPH and α-amylase inhibition assays were close to standard drugs, while the MTT assay demonstrated moderate cytotoxicity, confirming the extract’s safety. Glucose uptake increased significantly, particularly in L6 cells treated with the methanolic extract.Conclusion The polyherbal formulation exhibited potent anti-diabetic properties, particularly the methanolic extract, showing significant glucose regulation and insulin function improvement. This formulation holds promise as a natural treatment for diabetes. |
| format | Article |
| id | doaj-art-dccdabe2fd7443eca0f4a8e6b661f818 |
| institution | Kabale University |
| issn | 2056-5623 |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Future Science OA |
| spelling | doaj-art-dccdabe2fd7443eca0f4a8e6b661f8182025-08-20T03:28:33ZengTaylor & Francis GroupFuture Science OA2056-56232025-12-0111110.1080/20565623.2025.2468128Evaluation of polyherbal synergy against diabetes: in-vitro analysisSridevi N.0Thirumal M.1Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, IndiaDepartment of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Chengalpattu, Tamil Nadu, IndiaAims To evaluate the anti-diabetic properties of a polyherbal formulation consisting of Curcuma longa, Emblica officinalis, and Trigonella foenum-graecum through in-vitro assays, and investigate its synergistic effects on glucose metabolism, lipid metabolism, and insulin function.Materials and methods The polyherbal extract was prepared using methanol, water, and methanol-water as solvents. In-vitro antioxidant (DPPH, Phosphomolybdenum) and anti-diabetic (α-amylase, α-glucosidase inhibition, yeast glucose uptake, and inhibitory glucose diffusion) assays were conducted. L6 rat skeletal muscle cells were used for MTT assay and glucose uptake.Results The methanol extract showed the highest antioxidant and anti-diabetic activities compared to aqueous and hydroalcoholic extracts. The IC50 values in DPPH and α-amylase inhibition assays were close to standard drugs, while the MTT assay demonstrated moderate cytotoxicity, confirming the extract’s safety. Glucose uptake increased significantly, particularly in L6 cells treated with the methanolic extract.Conclusion The polyherbal formulation exhibited potent anti-diabetic properties, particularly the methanolic extract, showing significant glucose regulation and insulin function improvement. This formulation holds promise as a natural treatment for diabetes.https://www.tandfonline.com/doi/10.1080/20565623.2025.2468128Polyherbal formulationanti-diabeticCurcuma longaEmblica officinalisTrigonella foenum-graecumglucose uptake |
| spellingShingle | Sridevi N. Thirumal M. Evaluation of polyherbal synergy against diabetes: in-vitro analysis Future Science OA Polyherbal formulation anti-diabetic Curcuma longa Emblica officinalis Trigonella foenum-graecum glucose uptake |
| title | Evaluation of polyherbal synergy against diabetes: in-vitro analysis |
| title_full | Evaluation of polyherbal synergy against diabetes: in-vitro analysis |
| title_fullStr | Evaluation of polyherbal synergy against diabetes: in-vitro analysis |
| title_full_unstemmed | Evaluation of polyherbal synergy against diabetes: in-vitro analysis |
| title_short | Evaluation of polyherbal synergy against diabetes: in-vitro analysis |
| title_sort | evaluation of polyherbal synergy against diabetes in vitro analysis |
| topic | Polyherbal formulation anti-diabetic Curcuma longa Emblica officinalis Trigonella foenum-graecum glucose uptake |
| url | https://www.tandfonline.com/doi/10.1080/20565623.2025.2468128 |
| work_keys_str_mv | AT sridevin evaluationofpolyherbalsynergyagainstdiabetesinvitroanalysis AT thirumalm evaluationofpolyherbalsynergyagainstdiabetesinvitroanalysis |