Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i>
<i>Verticillium</i> spp. are soil-borne fungal pathogens capable of infecting over 400 plant species via their root systems, with each species demonstrating a distinct host range. The primary host of <i>Verticillium nonalfalfae</i> is the hop plant (<i>Humulus lupulus&l...
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2025-04-01
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| author | Seyedehtannaz Ghoreshizadeh Carla Calvo-Peña Marina Ruiz-Muñoz Maja Dobrajc Sebastjan Radišek Juan José R. Coque Rebeca Cobos |
| author_facet | Seyedehtannaz Ghoreshizadeh Carla Calvo-Peña Marina Ruiz-Muñoz Maja Dobrajc Sebastjan Radišek Juan José R. Coque Rebeca Cobos |
| author_sort | Seyedehtannaz Ghoreshizadeh |
| collection | DOAJ |
| description | <i>Verticillium</i> spp. are soil-borne fungal pathogens capable of infecting over 400 plant species via their root systems, with each species demonstrating a distinct host range. The primary host of <i>Verticillium nonalfalfae</i> is the hop plant (<i>Humulus lupulus</i>), which becomes affected by Verticillium wilt and, in severe cases, dieback. A dual screening approach was employed to isolate Streptomycetes as potential biocontrol agents from the hop root systems alongside other bacterial species from hop xylem. Three <i>Pseudomonas</i> strains from the xylem and three <i>Streptomyces</i> strains from the roots were selected based on their in vitro antifungal activity against <i>Verticillium dahliae</i> and/or <i>V. nonalfalfae</i>. Their potential for controlling Verticillium wilt was further assessed through in planta assays conducted under greenhouse conditions. The results demonstrated that <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 significantly reduced the disease severity index by 32.56% compared to artificially infected control plants. In contrast, the commercial biocontrol product Serenade<sup>®</sup> ASO, containing <i>Bacillus amyloliquefaciens</i> QST 713, achieved a reduction of 13.96%. These findings underscore the potential of the selected bacterial strains as promising candidates for the biological control of Verticillium wilt in hops affected by <i>V. nonalfalfae</i>. |
| format | Article |
| id | doaj-art-dcc747eaa8a044b6810a8750e90224d1 |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-dcc747eaa8a044b6810a8750e90224d12025-08-20T03:47:57ZengMDPI AGHorticulturae2311-75242025-04-0111545910.3390/horticulturae11050459Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i>Seyedehtannaz Ghoreshizadeh0Carla Calvo-Peña1Marina Ruiz-Muñoz2Maja Dobrajc3Sebastjan Radišek4Juan José R. Coque5Rebeca Cobos6Instituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainSlovenian Institute of Hop Research and Brewing, 3310 Žalec, SloveniaSlovenian Institute of Hop Research and Brewing, 3310 Žalec, SloveniaInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, SpainInstituto de Investigación de la Viña y el Vino, Escuela de Ingeniería Agraria, Universidad de León, 24009 León, Spain<i>Verticillium</i> spp. are soil-borne fungal pathogens capable of infecting over 400 plant species via their root systems, with each species demonstrating a distinct host range. The primary host of <i>Verticillium nonalfalfae</i> is the hop plant (<i>Humulus lupulus</i>), which becomes affected by Verticillium wilt and, in severe cases, dieback. A dual screening approach was employed to isolate Streptomycetes as potential biocontrol agents from the hop root systems alongside other bacterial species from hop xylem. Three <i>Pseudomonas</i> strains from the xylem and three <i>Streptomyces</i> strains from the roots were selected based on their in vitro antifungal activity against <i>Verticillium dahliae</i> and/or <i>V. nonalfalfae</i>. Their potential for controlling Verticillium wilt was further assessed through in planta assays conducted under greenhouse conditions. The results demonstrated that <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 significantly reduced the disease severity index by 32.56% compared to artificially infected control plants. In contrast, the commercial biocontrol product Serenade<sup>®</sup> ASO, containing <i>Bacillus amyloliquefaciens</i> QST 713, achieved a reduction of 13.96%. These findings underscore the potential of the selected bacterial strains as promising candidates for the biological control of Verticillium wilt in hops affected by <i>V. nonalfalfae</i>.https://www.mdpi.com/2311-7524/11/5/459antifungal activitybiocontrolhops<i>Humulus lupulus</i>rhizospheresoil-borne pathogen |
| spellingShingle | Seyedehtannaz Ghoreshizadeh Carla Calvo-Peña Marina Ruiz-Muñoz Maja Dobrajc Sebastjan Radišek Juan José R. Coque Rebeca Cobos Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i> Horticulturae antifungal activity biocontrol hops <i>Humulus lupulus</i> rhizosphere soil-borne pathogen |
| title | Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i> |
| title_full | Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i> |
| title_fullStr | Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i> |
| title_full_unstemmed | Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i> |
| title_short | Isolation and Characterization of <i>Pseudomonas</i> sp. HX1, <i>Streptomyces luteogriseus</i> HR40, and <i>Streptomyces flavofungini</i> HR77 as Promising Biocontrol Agents Against Verticillium Wilt in Hops Affected by <i>Verticillium nonalfalfae</i> |
| title_sort | isolation and characterization of i pseudomonas i sp hx1 i streptomyces luteogriseus i hr40 and i streptomyces flavofungini i hr77 as promising biocontrol agents against verticillium wilt in hops affected by i verticillium nonalfalfae i |
| topic | antifungal activity biocontrol hops <i>Humulus lupulus</i> rhizosphere soil-borne pathogen |
| url | https://www.mdpi.com/2311-7524/11/5/459 |
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