In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus
Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine worldwide and causes considerable economic loss. Identifying specific cell signaling or activation pathways that associate with variation in PRRSV replication and macrophage function may lead to identification o...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2010/864181 |
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| _version_ | 1841524807893516288 |
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| author | Laura C. Miller John D. Neill Gregory P. Harhay Kelly M. Lager William W. Laegreid Marcus E. Kehrli |
| author_facet | Laura C. Miller John D. Neill Gregory P. Harhay Kelly M. Lager William W. Laegreid Marcus E. Kehrli |
| author_sort | Laura C. Miller |
| collection | DOAJ |
| description | Porcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine worldwide and causes considerable economic loss. Identifying specific cell signaling or activation pathways that associate with variation in PRRSV replication and macrophage function may lead to identification of novel gene targets for the control of PRRSV infection. Serial Analysis of Gene Expression (SAGE) was used to create and survey the transcriptome of in vitro mock-infected and PRRSV strain VR-2332-infected porcine alveolar macrophages (PAM) at 0, 6, 12, 16, and 24 hours after infection. The transcriptome data indicated changes in transcript abundance occurring in PRRSV-infected PAMs over time after infection with more than 590 unique tags with significantly altered transcript abundance levels identified (P<.01). Strikingly, innate immune genes (whose transcript abundances are typically altered in response to other pathogens or insults including IL-8, CCL4, and IL-1β) showed no or very little change at any time point following infection. |
| format | Article |
| id | doaj-art-dcc235eb31ed4e77bf73bdb24bf4c5b1 |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-dcc235eb31ed4e77bf73bdb24bf4c5b12025-02-03T05:47:24ZengWileyAdvances in Virology1687-86391687-86472010-01-01201010.1155/2010/864181864181In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome VirusLaura C. Miller0John D. Neill1Gregory P. Harhay2Kelly M. Lager3William W. Laegreid4Marcus E. Kehrli5USDA, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, 1920 Dayton Ave, Ames, IA 50010, USAUSDA, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, 1920 Dayton Ave, Ames, IA 50010, USAUSDA, Agricultural Research Service, United States Meat Animal Research Center, Animal Health Research Unit, State Spur 18D, Clay Center, NE 68933, USAUSDA, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, 1920 Dayton Ave, Ames, IA 50010, USADepartment of Pathobiology, College of Veterinary Medicine, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61802, USAUSDA, Agricultural Research Service, National Animal Disease Center, Virus and Prion Research Unit, 1920 Dayton Ave, Ames, IA 50010, USAPorcine reproductive and respiratory syndrome virus (PRRSV) is a major pathogen of swine worldwide and causes considerable economic loss. Identifying specific cell signaling or activation pathways that associate with variation in PRRSV replication and macrophage function may lead to identification of novel gene targets for the control of PRRSV infection. Serial Analysis of Gene Expression (SAGE) was used to create and survey the transcriptome of in vitro mock-infected and PRRSV strain VR-2332-infected porcine alveolar macrophages (PAM) at 0, 6, 12, 16, and 24 hours after infection. The transcriptome data indicated changes in transcript abundance occurring in PRRSV-infected PAMs over time after infection with more than 590 unique tags with significantly altered transcript abundance levels identified (P<.01). Strikingly, innate immune genes (whose transcript abundances are typically altered in response to other pathogens or insults including IL-8, CCL4, and IL-1β) showed no or very little change at any time point following infection.http://dx.doi.org/10.1155/2010/864181 |
| spellingShingle | Laura C. Miller John D. Neill Gregory P. Harhay Kelly M. Lager William W. Laegreid Marcus E. Kehrli In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus Advances in Virology |
| title | In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus |
| title_full | In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus |
| title_fullStr | In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus |
| title_full_unstemmed | In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus |
| title_short | In-Depth Global Analysis of Transcript Abundance Levels in Porcine Alveolar Macrophages Following Infection with Porcine Reproductive and Respiratory Syndrome Virus |
| title_sort | in depth global analysis of transcript abundance levels in porcine alveolar macrophages following infection with porcine reproductive and respiratory syndrome virus |
| url | http://dx.doi.org/10.1155/2010/864181 |
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