MicroRNA Expression Profiling in Behçet’s Disease
Background. Behçet’s disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. MicroRNAs (miRNAs) are key regulators of immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune disea...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2018/2405150 |
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| author | Antonio Puccetti Andrea Pelosi Piera Filomena Fiore Giuseppe Patuzzo Claudio Lunardi Marzia Dolcino |
| author_facet | Antonio Puccetti Andrea Pelosi Piera Filomena Fiore Giuseppe Patuzzo Claudio Lunardi Marzia Dolcino |
| author_sort | Antonio Puccetti |
| collection | DOAJ |
| description | Background. Behçet’s disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. MicroRNAs (miRNAs) are key regulators of immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in BD is not fully elucidated. We aimed to identify miRNA expression signatures associated with BD and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray analysis was performed in blood cells of BD patients and healthy controls. miRNA expression profiles were analyzed using Affymetrix arrays with a comprehensive coverage of miRNA sequences. Pathway analyses were performed, and the global miRNA profiling was combined with transcriptoma data in BD. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with BD patients with active disease. These miRNAs target pathways relevant in BD, such as TNF, IFN gamma, and VEGF-VEGFR signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the BD transcriptoma. Conclusions. The combined analysis of deregulated miRNAs and BD transcriptome sheds light on some epigenetic aspects of BD identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in BD. |
| format | Article |
| id | doaj-art-dcbb515d7b7340cdb791c3b0b829857a |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-dcbb515d7b7340cdb791c3b0b829857a2025-02-03T01:11:48ZengWileyJournal of Immunology Research2314-88612314-71562018-01-01201810.1155/2018/24051502405150MicroRNA Expression Profiling in Behçet’s DiseaseAntonio Puccetti0Andrea Pelosi1Piera Filomena Fiore2Giuseppe Patuzzo3Claudio Lunardi4Marzia Dolcino5Immunology Area, Pediatric Hospital Bambino Gesù, Viale San Paolo 15, 00146 Rome, ItalyImmunology Area, Pediatric Hospital Bambino Gesù, Viale San Paolo 15, 00146 Rome, ItalyImmunology Area, Pediatric Hospital Bambino Gesù, Viale San Paolo 15, 00146 Rome, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyDepartment of Medicine, University of Verona, Piazzale L.A. Scuro 10, 37134 Verona, ItalyBackground. Behçet’s disease (BD) is a chronic inflammatory multisystem disease characterized by oral and genital ulcers, uveitis, and skin lesions. MicroRNAs (miRNAs) are key regulators of immune responses. Differential expression of miRNAs has been reported in several inflammatory autoimmune diseases; however, their role in BD is not fully elucidated. We aimed to identify miRNA expression signatures associated with BD and to investigate their potential implication in the disease pathogenesis. Methods. miRNA microarray analysis was performed in blood cells of BD patients and healthy controls. miRNA expression profiles were analyzed using Affymetrix arrays with a comprehensive coverage of miRNA sequences. Pathway analyses were performed, and the global miRNA profiling was combined with transcriptoma data in BD. Deregulation of selected miRNAs was validated by real-time PCR. Results. We identified specific miRNA signatures associated with BD patients with active disease. These miRNAs target pathways relevant in BD, such as TNF, IFN gamma, and VEGF-VEGFR signaling cascades. Network analysis revealed several miRNAs regulating highly connected genes within the BD transcriptoma. Conclusions. The combined analysis of deregulated miRNAs and BD transcriptome sheds light on some epigenetic aspects of BD identifying specific miRNAs, which may represent promising candidates as biomarkers and/or for the design of novel therapeutic strategies in BD.http://dx.doi.org/10.1155/2018/2405150 |
| spellingShingle | Antonio Puccetti Andrea Pelosi Piera Filomena Fiore Giuseppe Patuzzo Claudio Lunardi Marzia Dolcino MicroRNA Expression Profiling in Behçet’s Disease Journal of Immunology Research |
| title | MicroRNA Expression Profiling in Behçet’s Disease |
| title_full | MicroRNA Expression Profiling in Behçet’s Disease |
| title_fullStr | MicroRNA Expression Profiling in Behçet’s Disease |
| title_full_unstemmed | MicroRNA Expression Profiling in Behçet’s Disease |
| title_short | MicroRNA Expression Profiling in Behçet’s Disease |
| title_sort | microrna expression profiling in behcet s disease |
| url | http://dx.doi.org/10.1155/2018/2405150 |
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