RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis
Abstract Background Early identification of pathogenic bacteria and monitoring residual status are essential for accurate treatment of neonatal bacterial meningitis (NBM). Methods Clinical data and specimens were collected from neonates with NBM. Bacterial cultures and RT‐PCR of blood and cerebrospi...
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| Format: | Article |
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Wiley
2024-12-01
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| Series: | iLabmed |
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| Online Access: | https://doi.org/10.1002/ila2.55 |
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| author | Ying Li Ruiqi Xiao Peicen Zou Yue Du Qinglin Lu Jun Cui Yajuan Wang |
| author_facet | Ying Li Ruiqi Xiao Peicen Zou Yue Du Qinglin Lu Jun Cui Yajuan Wang |
| author_sort | Ying Li |
| collection | DOAJ |
| description | Abstract Background Early identification of pathogenic bacteria and monitoring residual status are essential for accurate treatment of neonatal bacterial meningitis (NBM). Methods Clinical data and specimens were collected from neonates with NBM. Bacterial cultures and RT‐PCR of blood and cerebrospinal fluid (CSF) were compared to assess the positivity rate, sensitivity and specificity of each method. Results RT‐PCR had a higher positivity rate compared with cultures, regardless of whether antibiotics had been used prior to specimen collection. After 1 week of regular antibiotic treatment, the number of pathogen DNA copy numbers in CSF was either undetectable or significantly reduced compared with previous levels. Conclusions RT‐PCR is expected to provide a basis for the precise application of antibiotics and the course of treatment for NBM, particularly in patients with negative cultures or those who have already been treated with antibiotics. |
| format | Article |
| id | doaj-art-dcac4cd126a6473db7dcbab9a5ead675 |
| institution | Kabale University |
| issn | 2834-443X 2834-4448 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | iLabmed |
| spelling | doaj-art-dcac4cd126a6473db7dcbab9a5ead6752025-08-20T04:01:16ZengWileyiLabmed2834-443X2834-44482024-12-012430731510.1002/ila2.55RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitisYing Li0Ruiqi Xiao1Peicen Zou2Yue Du3Qinglin Lu4Jun Cui5Yajuan Wang6Department of Neonatology Children's Hospital Capital Institute of Pediatrics Beijing ChinaCapital Institute of Pediatrics Beijing ChinaCapital Institute of Pediatrics Beijing ChinaDepartment of Neonatology Children's Hospital Capital Institute of Pediatrics Beijing ChinaChildren's Hospital Capital Institute of Pediatrics Chinese Academy of Medical Sciences & Peking Union Medical College Beijing ChinaDepartment of Neonatology Children's Hospital Capital Institute of Pediatrics Beijing ChinaDepartment of Neonatology Children's Hospital Capital Institute of Pediatrics Beijing ChinaAbstract Background Early identification of pathogenic bacteria and monitoring residual status are essential for accurate treatment of neonatal bacterial meningitis (NBM). Methods Clinical data and specimens were collected from neonates with NBM. Bacterial cultures and RT‐PCR of blood and cerebrospinal fluid (CSF) were compared to assess the positivity rate, sensitivity and specificity of each method. Results RT‐PCR had a higher positivity rate compared with cultures, regardless of whether antibiotics had been used prior to specimen collection. After 1 week of regular antibiotic treatment, the number of pathogen DNA copy numbers in CSF was either undetectable or significantly reduced compared with previous levels. Conclusions RT‐PCR is expected to provide a basis for the precise application of antibiotics and the course of treatment for NBM, particularly in patients with negative cultures or those who have already been treated with antibiotics.https://doi.org/10.1002/ila2.55bacterial meningitiscultureneonatepathogenRT‐PCR |
| spellingShingle | Ying Li Ruiqi Xiao Peicen Zou Yue Du Qinglin Lu Jun Cui Yajuan Wang RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis iLabmed bacterial meningitis culture neonate pathogen RT‐PCR |
| title | RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis |
| title_full | RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis |
| title_fullStr | RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis |
| title_full_unstemmed | RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis |
| title_short | RT‐PCR in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis |
| title_sort | rt pcr in the early detection and monitoring of pathogen residual status in neonatal bacterial meningitis |
| topic | bacterial meningitis culture neonate pathogen RT‐PCR |
| url | https://doi.org/10.1002/ila2.55 |
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