Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women

Background: Human papillomavirus (HPV) is considered one of sexually transferred disease in the world and it is considered as one of the causative agents of cervical cancer. Cervical cytology is used widely as the initial tool in cervical cancer screening worldwide. Objective: The purpose of this s...

Full description

Saved in:
Bibliographic Details
Main Authors: Nawres A. Tawfeeq, Thamer A. Hussein, Hussein A. Hasan, Haider G. Hussein, Wissam J. Mohammed, Dina S. Ibraheem
Format: Article
Language:English
Published: Al-Nahrain University/ Biotechnology Research Center 2025-04-01
Series:مجلة مركز بحوث التقنيات الاحيائية
Subjects:
Online Access:https://jobrc.org/index.php/jobrc/article/view/831
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850179029229895680
author Nawres A. Tawfeeq
Thamer A. Hussein
Hussein A. Hasan
Haider G. Hussein
Wissam J. Mohammed
Dina S. Ibraheem
author_facet Nawres A. Tawfeeq
Thamer A. Hussein
Hussein A. Hasan
Haider G. Hussein
Wissam J. Mohammed
Dina S. Ibraheem
author_sort Nawres A. Tawfeeq
collection DOAJ
description Background: Human papillomavirus (HPV) is considered one of sexually transferred disease in the world and it is considered as one of the causative agents of cervical cancer. Cervical cytology is used widely as the initial tool in cervical cancer screening worldwide. Objective: The purpose of this study was to analyze the correlation between cytological findings from Papanicolaou (pap.)-stained cervical smears and cases of HPV infection of the uterine cervix as identified by Polymerase Chain Reaction (PCR) testing. Methodology: Between June 2021 and December 2022, retrospective study for cervical smears of 370 Baghdad women (attending to the Central Public Health Laboratory (CPHL)/Ministry of Health in Baghdad) were stained by the Pap procedure and categorized by the Bethesda classification system, High Risk-Human papilloma Virus (HR-HPV) detection was used to analyze the HPV status of the cervical samples that were collected Results: HR-HPV testing was done for 370 women (230 women that have shown Positive results for HR-HPV and 140 were negative for HPV). Our result showed highly significant differences at (P ≤ 0.01) for HR-HPV genotype and abnormal Pap smear with High grade squamous intraepithelial lesion (HSIL) at 23.9 %, while it was at 41.3% with low grade squamous intraepithelial lesion (LSIL) Pap smear which represent the highest rate, in addition to 34.7% for abnormal Pap smear with atypical squamous cells of undetermined significance (ASCUS) classification Conclusion: This study has shown that molecular investigation for HR-HPV might be essential for diagnosis of patients with proven epithelial abnormality in their Pap smears.
format Article
id doaj-art-dca88f886bca4b49a93fa7ca91066078
institution OA Journals
issn 1815-1140
2708-1370
language English
publishDate 2025-04-01
publisher Al-Nahrain University/ Biotechnology Research Center
record_format Article
series مجلة مركز بحوث التقنيات الاحيائية
spelling doaj-art-dca88f886bca4b49a93fa7ca910660782025-08-20T02:18:35ZengAl-Nahrain University/ Biotechnology Research Centerمجلة مركز بحوث التقنيات الاحيائية1815-11402708-13702025-04-0119210.24126/jobrc.2025.19.2.831Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad womenNawres A. Tawfeeq0Thamer A. Hussein 1Hussein A. Hasan 2Haider G. Hussein 3Wissam J. Mohammed 4Dina S. Ibraheem Central Public Health Laboratory / Public Health Directorate/ Ministry of health/ Baghdad /Iraq Central Public Health Laboratory / Public Health Directorate/ Ministry of health/ Baghdad /Iraq Central Public Health Laboratory / Public Health Directorate/ Ministry of health/ Baghdad /Iraq Central Public Health Laboratory / Public Health Directorate/ Ministry of health/ Baghdad /Iraq Central Public Health Laboratory / Public Health Directorate/ Ministry of health/ Baghdad /Iraq Background: Human papillomavirus (HPV) is considered one of sexually transferred disease in the world and it is considered as one of the causative agents of cervical cancer. Cervical cytology is used widely as the initial tool in cervical cancer screening worldwide. Objective: The purpose of this study was to analyze the correlation between cytological findings from Papanicolaou (pap.)-stained cervical smears and cases of HPV infection of the uterine cervix as identified by Polymerase Chain Reaction (PCR) testing. Methodology: Between June 2021 and December 2022, retrospective study for cervical smears of 370 Baghdad women (attending to the Central Public Health Laboratory (CPHL)/Ministry of Health in Baghdad) were stained by the Pap procedure and categorized by the Bethesda classification system, High Risk-Human papilloma Virus (HR-HPV) detection was used to analyze the HPV status of the cervical samples that were collected Results: HR-HPV testing was done for 370 women (230 women that have shown Positive results for HR-HPV and 140 were negative for HPV). Our result showed highly significant differences at (P ≤ 0.01) for HR-HPV genotype and abnormal Pap smear with High grade squamous intraepithelial lesion (HSIL) at 23.9 %, while it was at 41.3% with low grade squamous intraepithelial lesion (LSIL) Pap smear which represent the highest rate, in addition to 34.7% for abnormal Pap smear with atypical squamous cells of undetermined significance (ASCUS) classification Conclusion: This study has shown that molecular investigation for HR-HPV might be essential for diagnosis of patients with proven epithelial abnormality in their Pap smears. https://jobrc.org/index.php/jobrc/article/view/831Human Papilloma virus (HPV)Pap smear, abnormal cervical findings (cytological changings)
spellingShingle Nawres A. Tawfeeq
Thamer A. Hussein
Hussein A. Hasan
Haider G. Hussein
Wissam J. Mohammed
Dina S. Ibraheem
Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women
مجلة مركز بحوث التقنيات الاحيائية
Human Papilloma virus (HPV)
Pap smear
, abnormal cervical findings (cytological changings)
title Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women
title_full Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women
title_fullStr Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women
title_full_unstemmed Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women
title_short Association between Human papillomavirus infection and abnormal cytopathology of uterine cervix in Baghdad women
title_sort association between human papillomavirus infection and abnormal cytopathology of uterine cervix in baghdad women
topic Human Papilloma virus (HPV)
Pap smear
, abnormal cervical findings (cytological changings)
url https://jobrc.org/index.php/jobrc/article/view/831
work_keys_str_mv AT nawresatawfeeq associationbetweenhumanpapillomavirusinfectionandabnormalcytopathologyofuterinecervixinbaghdadwomen
AT thamerahussein associationbetweenhumanpapillomavirusinfectionandabnormalcytopathologyofuterinecervixinbaghdadwomen
AT husseinahasan associationbetweenhumanpapillomavirusinfectionandabnormalcytopathologyofuterinecervixinbaghdadwomen
AT haiderghussein associationbetweenhumanpapillomavirusinfectionandabnormalcytopathologyofuterinecervixinbaghdadwomen
AT wissamjmohammed associationbetweenhumanpapillomavirusinfectionandabnormalcytopathologyofuterinecervixinbaghdadwomen
AT dinasibraheem associationbetweenhumanpapillomavirusinfectionandabnormalcytopathologyofuterinecervixinbaghdadwomen