Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction
Abstract Background Migratory insect infestation caused by Sogatella furcifera is a serious threat to rice production. The most effective method available for S. furcifera control is intensive insecticide spraying, which cause widespread resistance. RNA interference (RNAi) insecticides hold enormous...
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BMC
2024-11-01
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| Series: | Journal of Nanobiotechnology |
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| Online Access: | https://doi.org/10.1186/s12951-024-02966-8 |
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| author | Changwei Gong Wei Wang Yanxin Ma Xiaoxu Zhan Anchun Peng Jian Pu Jizhi Yang Xuegui Wang |
| author_facet | Changwei Gong Wei Wang Yanxin Ma Xiaoxu Zhan Anchun Peng Jian Pu Jizhi Yang Xuegui Wang |
| author_sort | Changwei Gong |
| collection | DOAJ |
| description | Abstract Background Migratory insect infestation caused by Sogatella furcifera is a serious threat to rice production. The most effective method available for S. furcifera control is intensive insecticide spraying, which cause widespread resistance. RNA interference (RNAi) insecticides hold enormous potential in managing pest resistance. However, the instability and the poor efficiency of cross-kingdom RNA trafficking are key obstacles for the application in agricultural pest management. Methods We present dendritic mesoporous silica nanoparticles (DMSNs)-based nanocarrier for delivering siRNA and nitenpyram to inhibit the metabolic detoxification and development of S. furcifera, thereby preventing its proliferation. Results This nano complex (denoted as N@UK-siRNA/DMSNs) significantly enhanced the stability of siRNA (efficacy lasting 21 days) and released cargos in GSH or planthopper bodily fluid with a maximum release rate of 84.99%. Moreover, the released UK-siRNA targeting two transcription factors (Ultraspiracle and Krüppel-homolog 1) downregulated the developmental genes Ultraspiracle (0.09-fold) and Krüppel-homolog 1 (0.284-fold), and downstream detoxification genes ABC SfABCH4 (0.016-fold) and P450 CYP6FJ3 (0.367-fold). Conclusion The N@UK-siRNA/DMSNs inhibited pest development and detoxification, significantly enhancing susceptibility to nitenpyram to nanogram level (LC50 is 250–252 ng/mL), resulting in a 5.37–7.13-fold synergistic ratio. This work proposes a comprehensive management strategy for controlling S. furcifera to ensure the green and safe production of rice. Graphical Abstract Schematic fabrication of the N@UK-siRNA/DMSNs and the molecular mechanism resolution of improving the sensitivity of S. furcifera to nitenpyram. The nitenpyram and UK-siRNA are assembled onto the DMSNs by interfacial modification. With phloem and xylem pathway, the N@UK-siRNA/DMSNs could transport bi-directionally to the feeding sites of S. furcifera and then release nitenpyram and UK-siRNA in S. furcifera in response to glutathione (GSH). The UK-siRNA released from N@UK-siRNA/DMSNs would downregulate the developmental genes USP and Kr-H1. The releasing nitenpyram would competitively bind to acetylcholine (ACh) receptors, inhibiting the transmission of nerve impulses |
| format | Article |
| id | doaj-art-dca80413df744adf8a01ea6c91ec567a |
| institution | DOAJ |
| issn | 1477-3155 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
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| series | Journal of Nanobiotechnology |
| spelling | doaj-art-dca80413df744adf8a01ea6c91ec567a2025-08-20T02:49:18ZengBMCJournal of Nanobiotechnology1477-31552024-11-0122112010.1186/s12951-024-02966-8Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproductionChangwei Gong0Wei Wang1Yanxin Ma2Xiaoxu Zhan3Anchun Peng4Jian Pu5Jizhi Yang6Xuegui Wang7State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural UniversityCollege of Science, Sichuan Agricultural UniversityCollege of Agriculture, Sichuan Agricultural UniversityInstitute of Urban Agriculture, Chinese Academy of Agricultural SciencesCollege of Agriculture, Sichuan Agricultural UniversityCollege of Agriculture, Sichuan Agricultural UniversityCollege of Agriculture, Sichuan Agricultural UniversityState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural UniversityAbstract Background Migratory insect infestation caused by Sogatella furcifera is a serious threat to rice production. The most effective method available for S. furcifera control is intensive insecticide spraying, which cause widespread resistance. RNA interference (RNAi) insecticides hold enormous potential in managing pest resistance. However, the instability and the poor efficiency of cross-kingdom RNA trafficking are key obstacles for the application in agricultural pest management. Methods We present dendritic mesoporous silica nanoparticles (DMSNs)-based nanocarrier for delivering siRNA and nitenpyram to inhibit the metabolic detoxification and development of S. furcifera, thereby preventing its proliferation. Results This nano complex (denoted as N@UK-siRNA/DMSNs) significantly enhanced the stability of siRNA (efficacy lasting 21 days) and released cargos in GSH or planthopper bodily fluid with a maximum release rate of 84.99%. Moreover, the released UK-siRNA targeting two transcription factors (Ultraspiracle and Krüppel-homolog 1) downregulated the developmental genes Ultraspiracle (0.09-fold) and Krüppel-homolog 1 (0.284-fold), and downstream detoxification genes ABC SfABCH4 (0.016-fold) and P450 CYP6FJ3 (0.367-fold). Conclusion The N@UK-siRNA/DMSNs inhibited pest development and detoxification, significantly enhancing susceptibility to nitenpyram to nanogram level (LC50 is 250–252 ng/mL), resulting in a 5.37–7.13-fold synergistic ratio. This work proposes a comprehensive management strategy for controlling S. furcifera to ensure the green and safe production of rice. Graphical Abstract Schematic fabrication of the N@UK-siRNA/DMSNs and the molecular mechanism resolution of improving the sensitivity of S. furcifera to nitenpyram. The nitenpyram and UK-siRNA are assembled onto the DMSNs by interfacial modification. With phloem and xylem pathway, the N@UK-siRNA/DMSNs could transport bi-directionally to the feeding sites of S. furcifera and then release nitenpyram and UK-siRNA in S. furcifera in response to glutathione (GSH). The UK-siRNA released from N@UK-siRNA/DMSNs would downregulate the developmental genes USP and Kr-H1. The releasing nitenpyram would competitively bind to acetylcholine (ACh) receptors, inhibiting the transmission of nerve impulseshttps://doi.org/10.1186/s12951-024-02966-8Sogatella furciferaInsecticide resistance managementRNAi pesticidesInsecticides deliveryDendritic mesoporous silica nanoparticles |
| spellingShingle | Changwei Gong Wei Wang Yanxin Ma Xiaoxu Zhan Anchun Peng Jian Pu Jizhi Yang Xuegui Wang Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction Journal of Nanobiotechnology Sogatella furcifera Insecticide resistance management RNAi pesticides Insecticides delivery Dendritic mesoporous silica nanoparticles |
| title | Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction |
| title_full | Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction |
| title_fullStr | Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction |
| title_full_unstemmed | Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction |
| title_short | Dendritic mesoporous silica-delivered siRNAs nano insecticides to prevent Sogatella furcifera by inhibiting metabolic detoxification and reproduction |
| title_sort | dendritic mesoporous silica delivered sirnas nano insecticides to prevent sogatella furcifera by inhibiting metabolic detoxification and reproduction |
| topic | Sogatella furcifera Insecticide resistance management RNAi pesticides Insecticides delivery Dendritic mesoporous silica nanoparticles |
| url | https://doi.org/10.1186/s12951-024-02966-8 |
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