Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis
Osteoarthritis (OA) is a slowly progressive joint disease typically seen in middle-age to elderly people. At present, there is no ideal agent to treat OA. Chenodeoxycholic acid (CDCA) was a principal active constituent from animal bile. However, the therapeutic effect of CDCA on OA severity was larg...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/780149 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849683381974990848 |
|---|---|
| author | Zhao-wei Yan Ji Dong Chen-hao Qin Chun-yang Zhao Li-yan Miao Chun-yan He |
| author_facet | Zhao-wei Yan Ji Dong Chen-hao Qin Chun-yang Zhao Li-yan Miao Chun-yan He |
| author_sort | Zhao-wei Yan |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a slowly progressive joint disease typically seen in middle-age to elderly people. At present, there is no ideal agent to treat OA. Chenodeoxycholic acid (CDCA) was a principal active constituent from animal bile. However, the therapeutic effect of CDCA on OA severity was largely unknown. The purpose of this study was to evaluate the therapeutic effect of intra-articular injection of CDCA in a rabbit OA model. OA was induced in experimental rabbits by anterior cruciate ligament transection (ACLT) and then rabbits were intra-articularly injected with CDCA (10 mg/kg or 50 mg/kg) once per week for 5 weeks. The results showed that CDCA significantly decreased cartilage degradation on the surface of femoral condyles, reducing the pathological changes of articular cartilage and synovial membrane by macroscopic and histological analysis. CDCA also significantly decreased bone destruction and erosion of joint evaluated by micro-CT. Furthermore, CDCA could markedly reduce the release of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), interleukin-1β (IL-1β), and prostaglandin E2 (PGE2) in synovial fluid. These observations highlight CDCA might be a potential therapeutic agent for OA. |
| format | Article |
| id | doaj-art-dca6ee2798304c0189f80c52ae7cb604 |
| institution | DOAJ |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-dca6ee2798304c0189f80c52ae7cb6042025-08-20T03:23:55ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/780149780149Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of OsteoarthritisZhao-wei Yan0Ji Dong1Chen-hao Qin2Chun-yang Zhao3Li-yan Miao4Chun-yan He5Department of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, ChinaDepartment of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, ChinaDepartment of Radiology, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, ChinaDepartment of Clinical Pharmacology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, ChinaDepartment of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215004, ChinaOsteoarthritis (OA) is a slowly progressive joint disease typically seen in middle-age to elderly people. At present, there is no ideal agent to treat OA. Chenodeoxycholic acid (CDCA) was a principal active constituent from animal bile. However, the therapeutic effect of CDCA on OA severity was largely unknown. The purpose of this study was to evaluate the therapeutic effect of intra-articular injection of CDCA in a rabbit OA model. OA was induced in experimental rabbits by anterior cruciate ligament transection (ACLT) and then rabbits were intra-articularly injected with CDCA (10 mg/kg or 50 mg/kg) once per week for 5 weeks. The results showed that CDCA significantly decreased cartilage degradation on the surface of femoral condyles, reducing the pathological changes of articular cartilage and synovial membrane by macroscopic and histological analysis. CDCA also significantly decreased bone destruction and erosion of joint evaluated by micro-CT. Furthermore, CDCA could markedly reduce the release of matrix metalloproteinase-1 (MMP-1), matrix metalloproteinase-3 (MMP-3), interleukin-1β (IL-1β), and prostaglandin E2 (PGE2) in synovial fluid. These observations highlight CDCA might be a potential therapeutic agent for OA.http://dx.doi.org/10.1155/2015/780149 |
| spellingShingle | Zhao-wei Yan Ji Dong Chen-hao Qin Chun-yang Zhao Li-yan Miao Chun-yan He Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis Mediators of Inflammation |
| title | Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis |
| title_full | Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis |
| title_fullStr | Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis |
| title_full_unstemmed | Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis |
| title_short | Therapeutic Effect of Chenodeoxycholic Acid in an Experimental Rabbit Model of Osteoarthritis |
| title_sort | therapeutic effect of chenodeoxycholic acid in an experimental rabbit model of osteoarthritis |
| url | http://dx.doi.org/10.1155/2015/780149 |
| work_keys_str_mv | AT zhaoweiyan therapeuticeffectofchenodeoxycholicacidinanexperimentalrabbitmodelofosteoarthritis AT jidong therapeuticeffectofchenodeoxycholicacidinanexperimentalrabbitmodelofosteoarthritis AT chenhaoqin therapeuticeffectofchenodeoxycholicacidinanexperimentalrabbitmodelofosteoarthritis AT chunyangzhao therapeuticeffectofchenodeoxycholicacidinanexperimentalrabbitmodelofosteoarthritis AT liyanmiao therapeuticeffectofchenodeoxycholicacidinanexperimentalrabbitmodelofosteoarthritis AT chunyanhe therapeuticeffectofchenodeoxycholicacidinanexperimentalrabbitmodelofosteoarthritis |