Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC
Despite tremendous efforts on isolation of pluripotent equine embryonic stem (ES) cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/429160 |
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| author | Khodadad Khodadadi Huseyin Sumer Maryam Pashaiasl Susan Lim Mark Williamson Paul J. Verma |
| author_facet | Khodadad Khodadadi Huseyin Sumer Maryam Pashaiasl Susan Lim Mark Williamson Paul J. Verma |
| author_sort | Khodadad Khodadadi |
| collection | DOAJ |
| description | Despite tremendous efforts on isolation of pluripotent equine embryonic stem (ES) cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts via retroviral transduction with three transcription factors using OCT4, SOX2, and KLF4 in the absence of c-MYC. The cell lines were maintained beyond 27 passages (more than 11 months) and characterized. The equine iPS (EiPS) cells stained positive for alkaline phosphatase by histochemical staining and expressed OCT4, NANOG, SSEA1, and SSEA4. Gene expression analysis of the cells showed the expression of OCT4, SOX2 NANOG, and STAT3. The cell lines retained a euploid chromosome count of 64 after long-term culture cryopreservation. The EiPS demonstrated differentiation capacity for the three embryonic germ layers both in vitro by embryoid bodies (EBs) formation and in vivo by teratoma formation. In conclusion, we report the derivation of iPS cells from equine adult fibroblasts and long-term maintenance using either of the three reprogramming factors. |
| format | Article |
| id | doaj-art-dca6744a7df14ae7978bd9b6af3cee5c |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-dca6744a7df14ae7978bd9b6af3cee5c2025-08-20T02:21:35ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/429160429160Induction of Pluripotency in Adult Equine Fibroblasts without c-MYCKhodadad Khodadadi0Huseyin Sumer1Maryam Pashaiasl2Susan Lim3Mark Williamson4Paul J. Verma5Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, VIC 3800, AustraliaCentre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, VIC 3800, AustraliaCentre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, VIC 3800, AustraliaStem Cell Technologies i (SCTi), Gleneagles Medical Centre, 258499, SingaporeGribbles Veterinary, Clayton, VIC 3168, AustraliaCentre for Reproduction and Development, Monash Institute of Medical Research, Monash University, Clayton, VIC 3800, AustraliaDespite tremendous efforts on isolation of pluripotent equine embryonic stem (ES) cells, to date there are few reports about successful isolation of ESCs and no report of in vivo differentiation of this important companion species. We report the induction of pluripotency in adult equine fibroblasts via retroviral transduction with three transcription factors using OCT4, SOX2, and KLF4 in the absence of c-MYC. The cell lines were maintained beyond 27 passages (more than 11 months) and characterized. The equine iPS (EiPS) cells stained positive for alkaline phosphatase by histochemical staining and expressed OCT4, NANOG, SSEA1, and SSEA4. Gene expression analysis of the cells showed the expression of OCT4, SOX2 NANOG, and STAT3. The cell lines retained a euploid chromosome count of 64 after long-term culture cryopreservation. The EiPS demonstrated differentiation capacity for the three embryonic germ layers both in vitro by embryoid bodies (EBs) formation and in vivo by teratoma formation. In conclusion, we report the derivation of iPS cells from equine adult fibroblasts and long-term maintenance using either of the three reprogramming factors.http://dx.doi.org/10.1155/2012/429160 |
| spellingShingle | Khodadad Khodadadi Huseyin Sumer Maryam Pashaiasl Susan Lim Mark Williamson Paul J. Verma Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC Stem Cells International |
| title | Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC |
| title_full | Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC |
| title_fullStr | Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC |
| title_full_unstemmed | Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC |
| title_short | Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC |
| title_sort | induction of pluripotency in adult equine fibroblasts without c myc |
| url | http://dx.doi.org/10.1155/2012/429160 |
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