Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins
10-Hydroxy-2-decenoic acid (10-HDA) is a medium-chain α,β-unsaturated carboxylic acid that exists in royal jelly with terminal hydroxylation. It has a broad market value because of its antibacterial, anti-inflammatory, anti-tumor, anti-radiation, and other active functions. The one-step whole-cell c...
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Frontiers Media S.A.
2025-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1590291/full |
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| author | Ziting Xu Chaofan Du Sheng Gao Xinrui Yan Pan Deng Yuehan Liu Junqing Wang Junqing Wang Ruiming Wang Ruiming Wang Nan Li Nan Li |
| author_facet | Ziting Xu Chaofan Du Sheng Gao Xinrui Yan Pan Deng Yuehan Liu Junqing Wang Junqing Wang Ruiming Wang Ruiming Wang Nan Li Nan Li |
| author_sort | Ziting Xu |
| collection | DOAJ |
| description | 10-Hydroxy-2-decenoic acid (10-HDA) is a medium-chain α,β-unsaturated carboxylic acid that exists in royal jelly with terminal hydroxylation. It has a broad market value because of its antibacterial, anti-inflammatory, anti-tumor, anti-radiation, and other active functions. The one-step whole-cell catalytic synthesis of 10-HDA by constructing engineered strains has improved the reaction rate to a certain extent compared with the previous two-step method. However, the accumulation of 10-HDA to a certain concentration in engineered Escherichia coli strains will damage the structure and function of cells and even lead to death; this unique antibacterial and antimicrobial activity seriously constrains the production of 10-HDA. In this study, we mined a transporter protein from Pseudomonas aeruginosa, which possesses the ability to efficiently efflux 10-HDA, and constructed a transporter protein overexpression strain by using the multicopy chromosome integration technique, which further improved the efficiency of product efflux, weakened the feedback inhibition of 10-HDA to a certain degree, and increased the substrate conversion rate to 88.6%. 10-HDA was synthesized up to 0.94 g/L by the replenishment flow-addition technique, providing a simple and efficient pathway for the yield breakthrough of 10-HDA biosynthesis. |
| format | Article |
| id | doaj-art-dca28eacc4904e6c852e506894d8f233 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-dca28eacc4904e6c852e506894d8f2332025-08-20T03:45:47ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-06-011310.3389/fbioe.2025.15902911590291Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteinsZiting Xu0Chaofan Du1Sheng Gao2Xinrui Yan3Pan Deng4Yuehan Liu5Junqing Wang6Junqing Wang7Ruiming Wang8Ruiming Wang9Nan Li10Nan Li11School of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, Shandong, ChinaSchool of Bioengineering, Qilu University of Technology, Jinan, Shandong, ChinaState Key Laboratory of Green Papermaking and Resource Recycling, Qilu University of Technology, Jinan, Shandong, China10-Hydroxy-2-decenoic acid (10-HDA) is a medium-chain α,β-unsaturated carboxylic acid that exists in royal jelly with terminal hydroxylation. It has a broad market value because of its antibacterial, anti-inflammatory, anti-tumor, anti-radiation, and other active functions. The one-step whole-cell catalytic synthesis of 10-HDA by constructing engineered strains has improved the reaction rate to a certain extent compared with the previous two-step method. However, the accumulation of 10-HDA to a certain concentration in engineered Escherichia coli strains will damage the structure and function of cells and even lead to death; this unique antibacterial and antimicrobial activity seriously constrains the production of 10-HDA. In this study, we mined a transporter protein from Pseudomonas aeruginosa, which possesses the ability to efficiently efflux 10-HDA, and constructed a transporter protein overexpression strain by using the multicopy chromosome integration technique, which further improved the efficiency of product efflux, weakened the feedback inhibition of 10-HDA to a certain degree, and increased the substrate conversion rate to 88.6%. 10-HDA was synthesized up to 0.94 g/L by the replenishment flow-addition technique, providing a simple and efficient pathway for the yield breakthrough of 10-HDA biosynthesis.https://www.frontiersin.org/articles/10.3389/fbioe.2025.1590291/fulltransporter proteinschromosome integrationmetabolic engineeringwhole-cell catalysismedium-chain fatty acid |
| spellingShingle | Ziting Xu Chaofan Du Sheng Gao Xinrui Yan Pan Deng Yuehan Liu Junqing Wang Junqing Wang Ruiming Wang Ruiming Wang Nan Li Nan Li Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins Frontiers in Bioengineering and Biotechnology transporter proteins chromosome integration metabolic engineering whole-cell catalysis medium-chain fatty acid |
| title | Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins |
| title_full | Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins |
| title_fullStr | Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins |
| title_full_unstemmed | Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins |
| title_short | Enhancing 10-HDA production of Escherichia coli by heterologous expression of MexHID transporter proteins |
| title_sort | enhancing 10 hda production of escherichia coli by heterologous expression of mexhid transporter proteins |
| topic | transporter proteins chromosome integration metabolic engineering whole-cell catalysis medium-chain fatty acid |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2025.1590291/full |
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