Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection
Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes. Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age <36 years, male factor infertility, long protocol using GnRH agonist...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Obstetrics and Gynecology International |
| Online Access: | http://dx.doi.org/10.1155/2012/403531 |
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| author | Catherine Patrat Aida Kaffel Lucie Delaroche Juliette Guibert Pierre Jouannet Sylvie Epelboin Dominique De Ziegler Jean-Philippe Wolf Patricia Fauque |
| author_facet | Catherine Patrat Aida Kaffel Lucie Delaroche Juliette Guibert Pierre Jouannet Sylvie Epelboin Dominique De Ziegler Jean-Philippe Wolf Patricia Fauque |
| author_sort | Catherine Patrat |
| collection | DOAJ |
| description | Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes.
Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age <36 years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (𝑛=110). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation (𝑇1) and the time between denudation and ICSI (𝑇2) using a statistical logistic regression analysis. Results. Neither 𝑇1 nor 𝑇2 had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when 𝑇1 was longer (optimal results at 𝑇1=3
hours) while FR significantly decreased with the increase of 𝑇2. Optimal implantation (IR) and pregnancy (PR) rates were obtained when 𝑇1 was around 2 hours.
Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR. |
| format | Article |
| id | doaj-art-dc970a4f6a9745889376f2ff73a2efbc |
| institution | Kabale University |
| issn | 1687-9589 1687-9597 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Obstetrics and Gynecology International |
| spelling | doaj-art-dc970a4f6a9745889376f2ff73a2efbc2025-02-03T05:58:55ZengWileyObstetrics and Gynecology International1687-95891687-95972012-01-01201210.1155/2012/403531403531Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm InjectionCatherine Patrat0Aida Kaffel1Lucie Delaroche2Juliette Guibert3Pierre Jouannet4Sylvie Epelboin5Dominique De Ziegler6Jean-Philippe Wolf7Patricia Fauque8Laboratoire de Biologie de la Reproduction, AP-HP, Hôpital Bichat Université Paris Diderot, 46 Rue Henri Huchard, 75018 Paris, FranceLaboratoire de Biologie de la Reproduction, AP-HP, Hôpital Cochin, 75014 Paris, FranceLaboratoire de Biologie de la Reproduction, AP-HP, Hôpital Bichat Université Paris Diderot, 46 Rue Henri Huchard, 75018 Paris, FranceService de Gynécologie-Obstétrique, Institut Mutualiste Monstsouris, 75014 Paris, FranceLaboratoire de Biologie de la Reproduction, AP-HP, Hôpital Cochin, 75014 Paris, FranceService de Gynécologie-Obstétrique II, AP-HP, Hôpital Bichat, 75018 Paris, FranceService de Gynécologie-Obstétrique II, AP-HP, Hôpital Cochin, Université Paris Descartes, 75005 Paris, FranceLaboratoire de Biologie de la Reproduction, AP-HP, Hôpital Cochin, 75014 Paris, FranceLaboratoire de Biologie de la reproduction CECOS, CHU de Dijon, Université de Bourgogne, Dijon, FranceObjectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes. Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age <36 years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (𝑛=110). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation (𝑇1) and the time between denudation and ICSI (𝑇2) using a statistical logistic regression analysis. Results. Neither 𝑇1 nor 𝑇2 had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when 𝑇1 was longer (optimal results at 𝑇1=3 hours) while FR significantly decreased with the increase of 𝑇2. Optimal implantation (IR) and pregnancy (PR) rates were obtained when 𝑇1 was around 2 hours. Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR.http://dx.doi.org/10.1155/2012/403531 |
| spellingShingle | Catherine Patrat Aida Kaffel Lucie Delaroche Juliette Guibert Pierre Jouannet Sylvie Epelboin Dominique De Ziegler Jean-Philippe Wolf Patricia Fauque Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection Obstetrics and Gynecology International |
| title | Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection |
| title_full | Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection |
| title_fullStr | Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection |
| title_full_unstemmed | Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection |
| title_short | Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection |
| title_sort | optimal timing for oocyte denudation and intracytoplasmic sperm injection |
| url | http://dx.doi.org/10.1155/2012/403531 |
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