Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system
Folate receptor alpha (FRα) has been regarded as a promising target for ovarian cancer (OvCa) therapy. This work focused on improving the identification of inhibitory aptamers that specifically target and block FRα. A new integrated microfluidic system (IMS) was designed to automate the entire syste...
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| Language: | English |
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Elsevier
2024-11-01
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| Series: | Chemical Engineering Journal Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266682112400098X |
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| author | Yi-Cheng Tsai Yang-Sheng Shao Chih-Hung Wang Keng-Fu Hsu Gwo-Bin Lee |
| author_facet | Yi-Cheng Tsai Yang-Sheng Shao Chih-Hung Wang Keng-Fu Hsu Gwo-Bin Lee |
| author_sort | Yi-Cheng Tsai |
| collection | DOAJ |
| description | Folate receptor alpha (FRα) has been regarded as a promising target for ovarian cancer (OvCa) therapy. This work focused on improving the identification of inhibitory aptamers that specifically target and block FRα. A new integrated microfluidic system (IMS) was designed to automate the entire systematic evolution of ligands by exponential enrichment (SELEX), precisely controlling the washing shear force from 62.7 nN to 451.7 nN that gradually washed away low-affinity aptamers, allowing high-affinity, high-specificity, single-stranded DNA aptamers to be screened within 15 h, which is significantly shorter than conventional process (weeks to months) while consuming 50 % less samples and reagents. Furthermore, screened aptamers significantly inhibited OvCa progression, achieving 20 % higher wound recovery in wound-healing tests when compared with an anti-cancer drug targeting FRα. In summary, precisely controlled shear force by IMS could optimize aptamers screening with high affinity/specificity towards FRα, which inhibited OvCa cell growth, suggesting their applicability as promising candidates for onco-therapy. |
| format | Article |
| id | doaj-art-dc8f74eb360940f7ab286ee21e6c13b8 |
| institution | OA Journals |
| issn | 2666-8211 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chemical Engineering Journal Advances |
| spelling | doaj-art-dc8f74eb360940f7ab286ee21e6c13b82025-08-20T02:30:34ZengElsevierChemical Engineering Journal Advances2666-82112024-11-012010068110.1016/j.ceja.2024.100681Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic systemYi-Cheng Tsai0Yang-Sheng Shao1Chih-Hung Wang2Keng-Fu Hsu3Gwo-Bin Lee4Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, TaiwanDepartment of Obstetrics and Gynecology, National Cheng Kung University, Tainan, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu, Taiwan; Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, Taiwan; Corresponding author.Folate receptor alpha (FRα) has been regarded as a promising target for ovarian cancer (OvCa) therapy. This work focused on improving the identification of inhibitory aptamers that specifically target and block FRα. A new integrated microfluidic system (IMS) was designed to automate the entire systematic evolution of ligands by exponential enrichment (SELEX), precisely controlling the washing shear force from 62.7 nN to 451.7 nN that gradually washed away low-affinity aptamers, allowing high-affinity, high-specificity, single-stranded DNA aptamers to be screened within 15 h, which is significantly shorter than conventional process (weeks to months) while consuming 50 % less samples and reagents. Furthermore, screened aptamers significantly inhibited OvCa progression, achieving 20 % higher wound recovery in wound-healing tests when compared with an anti-cancer drug targeting FRα. In summary, precisely controlled shear force by IMS could optimize aptamers screening with high affinity/specificity towards FRα, which inhibited OvCa cell growth, suggesting their applicability as promising candidates for onco-therapy.http://www.sciencedirect.com/science/article/pii/S266682112400098XSELEXInhibitory aptamersMicrofluidicsOvarian cancerShear force |
| spellingShingle | Yi-Cheng Tsai Yang-Sheng Shao Chih-Hung Wang Keng-Fu Hsu Gwo-Bin Lee Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system Chemical Engineering Journal Advances SELEX Inhibitory aptamers Microfluidics Ovarian cancer Shear force |
| title | Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system |
| title_full | Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system |
| title_fullStr | Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system |
| title_full_unstemmed | Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system |
| title_short | Optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated, shear force-controlling microfluidic system |
| title_sort | optimizing the screening process for inhibitory aptamers specific to folate receptor alpha on an integrated shear force controlling microfluidic system |
| topic | SELEX Inhibitory aptamers Microfluidics Ovarian cancer Shear force |
| url | http://www.sciencedirect.com/science/article/pii/S266682112400098X |
| work_keys_str_mv | AT yichengtsai optimizingthescreeningprocessforinhibitoryaptamersspecifictofolatereceptoralphaonanintegratedshearforcecontrollingmicrofluidicsystem AT yangshengshao optimizingthescreeningprocessforinhibitoryaptamersspecifictofolatereceptoralphaonanintegratedshearforcecontrollingmicrofluidicsystem AT chihhungwang optimizingthescreeningprocessforinhibitoryaptamersspecifictofolatereceptoralphaonanintegratedshearforcecontrollingmicrofluidicsystem AT kengfuhsu optimizingthescreeningprocessforinhibitoryaptamersspecifictofolatereceptoralphaonanintegratedshearforcecontrollingmicrofluidicsystem AT gwobinlee optimizingthescreeningprocessforinhibitoryaptamersspecifictofolatereceptoralphaonanintegratedshearforcecontrollingmicrofluidicsystem |