The Effect of Water Content of Medium Containing Oryctes rhinoceros Larvae on Metarhizium anisopliae Pathogenicity
<p class="IsiAbstrakIndo"><span lang="EN-GB">The entomopathogenic fungus, </span><em><span lang="EN-GB">Metarhizium anisopliae</span></em><span lang="EN-GB"> (Metschnikoff) Sorokin (Ascomycota: Hypocreales)</s...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Negeri Semarang
2017-08-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
| Subjects: | |
| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/10262 |
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| Summary: | <p class="IsiAbstrakIndo"><span lang="EN-GB">The entomopathogenic fungus, </span><em><span lang="EN-GB">Metarhizium anisopliae</span></em><span lang="EN-GB"> (Metschnikoff) Sorokin (Ascomycota: Hypocreales)</span><em></em><span lang="EN-GB">would effectively infect the target host on the appropriate medium water content. The aim of this study was to analyze the influence of water content of medium on the effectiveness of </span><em><span lang="EN-GB">M. anisopliae </span></em><span lang="EN-GB">fungus infection on </span><em><span lang="EN-GB">O. rhinoceros</span></em><span lang="EN-GB"> larvae in the laboratory. Fifty healthy third instar larvae of </span><em><span lang="EN-GB">O. rhinoceros</span></em><span lang="EN-GB"> were obtained from field. The </span><em><span lang="EN-GB">M. anisopliae </span></em><span lang="EN-GB">obtained from Estate Crop Protection Board in Salatiga. The conidia density and viability of </span><em><span lang="EN-GB">M. anisopliae </span></em><span lang="EN-GB">were examined before used. The medium for maintaining the larva was the sawdust that had been sterilized. A total of 50 plastic cups were prepared to place 50 larvae (1 larva/cup). Each cup was filled with 100 g medium of sawdust plus 2 g of </span><em><span lang="EN-GB">M. anisopliae </span></em><span lang="EN-GB">which was then stirred until mixed, with different water content: P1 (20%), P2 (40%), P3 (60%), P4 (80% ) and P5 (98%). The result indicated that the water content of the medium affected the effectiveness of </span><em><span lang="EN-GB">M. anisopliae </span></em><span lang="EN-GB">fungus infection on </span><em><span lang="EN-GB">O. rhinoceros</span></em><span lang="EN-GB"> larvae. The water content influenced the duration of larval mortality at each treatment. An important finding in this study is that controlling </span><em><span lang="EN-GB">O. rhineceros</span></em><span lang="EN-GB"> larvae with </span><em><span lang="EN-GB">M. anisopliae</span></em><span lang="EN-GB"> can be done by manipulating the water content of medium. The benefit of this study may be used for the recommendation of </span><em><span lang="EN-GB">O. rhinoceros</span></em><span lang="EN-GB"> pest control using </span><em><span lang="EN-GB">M. anisopliae </span></em><span lang="EN-GB">with an effective water media content.</span></p> |
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| ISSN: | 2085-191X 2338-7610 |