Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice
MYH14 is a member of the myosin family, which has been implicated in many motile processes such as ion-channel gating, organelle translocation, and the cytoskeleton rearrangement. Mutations in MYH14 lead to a DFNA4-type hearing impairment. Further evidence also shows that MYH14 is a candidate noise-...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/2016/6720420 |
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| author | Xiaolong Fu Linqing Zhang Yecheng Jin Xiaoyang Sun Aizhen Zhang Zongzhuang Wen Yichen Zhou Ming Xia Jiangang Gao |
| author_facet | Xiaolong Fu Linqing Zhang Yecheng Jin Xiaoyang Sun Aizhen Zhang Zongzhuang Wen Yichen Zhou Ming Xia Jiangang Gao |
| author_sort | Xiaolong Fu |
| collection | DOAJ |
| description | MYH14 is a member of the myosin family, which has been implicated in many motile processes such as ion-channel gating, organelle translocation, and the cytoskeleton rearrangement. Mutations in MYH14 lead to a DFNA4-type hearing impairment. Further evidence also shows that MYH14 is a candidate noise-induced hearing loss (NIHL) susceptible gene. However, the specific roles of MYH14 in auditory function and NIHL are not fully understood. In the present study, we used CRISPR/Cas9 technology to establish a Myh14 knockout mice line in CBA/CaJ background (now referred to as Myh14−/− mice) and clarify the role of MYH14 in the cochlea and NIHL. We found that Myh14−/− mice did not exhibit significant hearing loss until five months of age. In addition, Myh14−/− mice were more vulnerable to high intensity noise compared to control mice. More significant outer hair cell loss was observed in Myh14−/− mice than in wild type controls after acoustic trauma. Our findings suggest that Myh14 may play a beneficial role in the protection of the cochlea after acoustic overstimulation in CBA/CaJ mice. |
| format | Article |
| id | doaj-art-dc88d418c6f94ed6bea96a789175dfad |
| institution | Kabale University |
| issn | 2090-5904 1687-5443 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Neural Plasticity |
| spelling | doaj-art-dc88d418c6f94ed6bea96a789175dfad2025-02-03T01:30:59ZengWileyNeural Plasticity2090-59041687-54432016-01-01201610.1155/2016/67204206720420Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ MiceXiaolong Fu0Linqing Zhang1Yecheng Jin2Xiaoyang Sun3Aizhen Zhang4Zongzhuang Wen5Yichen Zhou6Ming Xia7Jiangang Gao8School of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaDepartment of Otolaryngology-Head and Neck Surgery, The Second Hospital of Shandong University, Jinan 250033, ChinaSchool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology, Shandong University, Jinan 250100, ChinaMYH14 is a member of the myosin family, which has been implicated in many motile processes such as ion-channel gating, organelle translocation, and the cytoskeleton rearrangement. Mutations in MYH14 lead to a DFNA4-type hearing impairment. Further evidence also shows that MYH14 is a candidate noise-induced hearing loss (NIHL) susceptible gene. However, the specific roles of MYH14 in auditory function and NIHL are not fully understood. In the present study, we used CRISPR/Cas9 technology to establish a Myh14 knockout mice line in CBA/CaJ background (now referred to as Myh14−/− mice) and clarify the role of MYH14 in the cochlea and NIHL. We found that Myh14−/− mice did not exhibit significant hearing loss until five months of age. In addition, Myh14−/− mice were more vulnerable to high intensity noise compared to control mice. More significant outer hair cell loss was observed in Myh14−/− mice than in wild type controls after acoustic trauma. Our findings suggest that Myh14 may play a beneficial role in the protection of the cochlea after acoustic overstimulation in CBA/CaJ mice.http://dx.doi.org/10.1155/2016/6720420 |
| spellingShingle | Xiaolong Fu Linqing Zhang Yecheng Jin Xiaoyang Sun Aizhen Zhang Zongzhuang Wen Yichen Zhou Ming Xia Jiangang Gao Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice Neural Plasticity |
| title | Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice |
| title_full | Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice |
| title_fullStr | Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice |
| title_full_unstemmed | Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice |
| title_short | Loss of Myh14 Increases Susceptibility to Noise-Induced Hearing Loss in CBA/CaJ Mice |
| title_sort | loss of myh14 increases susceptibility to noise induced hearing loss in cba caj mice |
| url | http://dx.doi.org/10.1155/2016/6720420 |
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