Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria
Abstract Background Exosomes are rich in bioactive molecules. Their unique lipid bilayer structure gives them the advantages of protecting the contents from degradation, easy cellular uptake, and good passive targeting. MSC-derived exosomes, which are natural vesicles ideal for gene delivery, exhibi...
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| Format: | Article |
| Language: | English |
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BMC
2025-07-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-025-04517-w |
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| author | YaJun Ma Tao Liu Pujun Li Lei Cao Xiangyu Lu HongBin Wang |
| author_facet | YaJun Ma Tao Liu Pujun Li Lei Cao Xiangyu Lu HongBin Wang |
| author_sort | YaJun Ma |
| collection | DOAJ |
| description | Abstract Background Exosomes are rich in bioactive molecules. Their unique lipid bilayer structure gives them the advantages of protecting the contents from degradation, easy cellular uptake, and good passive targeting. MSC-derived exosomes, which are natural vesicles ideal for gene delivery, exhibit a broad spectrum of therapeutic benefits. Methods In this study, which combines cell-free therapy with gene therapy, adipose mesenchymal stem cells (ADSCs) were genetically modified with augmenter of liver regeneration (ALR) to derive exosomes overexpressing ALR, which were used as therapeutic agents in a miniature porcine model of laparoscopic hepatic ischemia‒reperfusion injury (IRI) combined with partial hepatectomy injury. Results The findings of this study demonstrated that ALR-overexpressing adipose mesenchymal stem cell-derived exosomes (ADSC-Exo) ameliorate hepatic tissue injury, reduce apoptosis, promote hepatic regeneration, and protect the integrity of the cellular mitochondrial structure and function. Conclusions In conclusion, ALR gene-modified ADSC-Exo strongly attenuate liver injury and promote liver tissue repair. |
| format | Article |
| id | doaj-art-dc76c911fd724c7d88c575c19336798e |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-dc76c911fd724c7d88c575c19336798e2025-08-20T03:46:03ZengBMCStem Cell Research & Therapy1757-65122025-07-0116111610.1186/s13287-025-04517-wExosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondriaYaJun Ma0Tao Liu1Pujun Li2Lei Cao3Xiangyu Lu4HongBin Wang5College of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityCollege of Veterinary Medicine, Northeast Agricultural UniversityAbstract Background Exosomes are rich in bioactive molecules. Their unique lipid bilayer structure gives them the advantages of protecting the contents from degradation, easy cellular uptake, and good passive targeting. MSC-derived exosomes, which are natural vesicles ideal for gene delivery, exhibit a broad spectrum of therapeutic benefits. Methods In this study, which combines cell-free therapy with gene therapy, adipose mesenchymal stem cells (ADSCs) were genetically modified with augmenter of liver regeneration (ALR) to derive exosomes overexpressing ALR, which were used as therapeutic agents in a miniature porcine model of laparoscopic hepatic ischemia‒reperfusion injury (IRI) combined with partial hepatectomy injury. Results The findings of this study demonstrated that ALR-overexpressing adipose mesenchymal stem cell-derived exosomes (ADSC-Exo) ameliorate hepatic tissue injury, reduce apoptosis, promote hepatic regeneration, and protect the integrity of the cellular mitochondrial structure and function. Conclusions In conclusion, ALR gene-modified ADSC-Exo strongly attenuate liver injury and promote liver tissue repair.https://doi.org/10.1186/s13287-025-04517-wCell-free therapyGene therapyADSC-ExoALRMiniature porcineHepatic IRI |
| spellingShingle | YaJun Ma Tao Liu Pujun Li Lei Cao Xiangyu Lu HongBin Wang Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria Stem Cell Research & Therapy Cell-free therapy Gene therapy ADSC-Exo ALR Miniature porcine Hepatic IRI |
| title | Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria |
| title_full | Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria |
| title_fullStr | Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria |
| title_full_unstemmed | Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria |
| title_short | Exosomes derived from ALR-modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia–reperfusion injury by promoting regeneration and protecting mitochondria |
| title_sort | exosomes derived from alr modified adipose mesenchymal stem cells mediate hepatoprotective effects on hepatic ischemia reperfusion injury by promoting regeneration and protecting mitochondria |
| topic | Cell-free therapy Gene therapy ADSC-Exo ALR Miniature porcine Hepatic IRI |
| url | https://doi.org/10.1186/s13287-025-04517-w |
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