Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis
Aim: Candidemia may occur when endogenous Candida species in the intestinal microbiota enter the bloodstream in patients with risk factors. Apart from endogenous transmission, patients can also transmit it exogenously. In the current study, we aimed to investigate the fluconazole susceptibility and...
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Galenos Publishing House
2025-03-01
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| Series: | Haseki Tıp Bülteni |
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| Online Access: | https://www.hasekidergisi.com/articles/investigation-of-clonal-relationship-between-lessemgreatercandida-parapsilosislessemgreater-and-lessemgreatercandida-glabratalessemgreater-strains-isolated-from-blood-culture-by-pulse-field-gel-electrophoresis/doi/haseki.galenos.2025.36844 |
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| author | Ayse Alici Elif Seren Tanriverdi Gulgun Yenisehirli Baris Otlu |
| author_facet | Ayse Alici Elif Seren Tanriverdi Gulgun Yenisehirli Baris Otlu |
| author_sort | Ayse Alici |
| collection | DOAJ |
| description | Aim: Candidemia may occur when endogenous Candida species in the intestinal microbiota enter the bloodstream in patients with risk factors. Apart from endogenous transmission, patients can also transmit it exogenously. In the current study, we aimed to investigate the fluconazole susceptibility and genotypes of Candida parapsilosis (C.parapsilosis) and Candida glabrata (C. glabrata) strains that cause candidemia.
Methods: Twenty-six C. parapsilosis and sixteen C. glabrata strains were included in the study. Fluconazole sensitivity was determined by the broth microdilution method. Genotyping of strains was done by pulsed-field gel electrophoresis.
Results: Nine (34%) of C. parapsilosis strains and 3 (19%) of C. glabrata strains were found to be resistant to fluconazole. Among 26 C. parapsilosis isolates, 17 different genotypes were detected and clustered isolates were collected in five clusters. Fourteen out of the 26 C. parapsilosis isolates are placed in one of the clusters, with a clustering rate of 53.8%. Among 16 C. glabrata isolates, 11 different genotypes were detected and divided into four clusters. Nine out of the 16 C. glabrata isolates are placed in one of the clusters, with a clustering rate of 56.2%.
Conclusion: Our data indicated the possibility of nosocomial transmission of C. parapsilosis and C. glabrata among intensive care unit patients in our hospital. Infection control policies should be strictly applied in our hospital to prevent cross-transmission. |
| format | Article |
| id | doaj-art-dc6aa80c5a0643039f3cec01ce1520a9 |
| institution | Kabale University |
| issn | 1302-0072 2147-2688 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Haseki Tıp Bülteni |
| spelling | doaj-art-dc6aa80c5a0643039f3cec01ce1520a92025-08-20T03:44:41ZengGalenos Publishing HouseHaseki Tıp Bülteni1302-00722147-26882025-03-01632626810.4274/haseki.galenos.2025.36844Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel ElectrophoresisAyse Alici0https://orcid.org/0000-0003-3709-1332Elif Seren Tanriverdi1https://orcid.org/0000-0002-0449-0356Gulgun Yenisehirli2https://orcid.org/0000-0001-7030-0752Baris Otlu3https://orcid.org/0000-0002-6220-0521Tavsanli Doc. Dr. M. Kalemli State Hospital, Clinic of Medical Microbiology, Kutahya, TurkeyMalatya Training and Research Hospital, Clinic of Medical Microbiology, Malatya, TurkeyTokat Gaziosmanpasa University Faculty of Medicine, Department of Medical Microbiology, Tokat, TurkeyInonu University Faculty of Medicine, Department of Medical Microbiology, Malatya, TurkeyAim: Candidemia may occur when endogenous Candida species in the intestinal microbiota enter the bloodstream in patients with risk factors. Apart from endogenous transmission, patients can also transmit it exogenously. In the current study, we aimed to investigate the fluconazole susceptibility and genotypes of Candida parapsilosis (C.parapsilosis) and Candida glabrata (C. glabrata) strains that cause candidemia. Methods: Twenty-six C. parapsilosis and sixteen C. glabrata strains were included in the study. Fluconazole sensitivity was determined by the broth microdilution method. Genotyping of strains was done by pulsed-field gel electrophoresis. Results: Nine (34%) of C. parapsilosis strains and 3 (19%) of C. glabrata strains were found to be resistant to fluconazole. Among 26 C. parapsilosis isolates, 17 different genotypes were detected and clustered isolates were collected in five clusters. Fourteen out of the 26 C. parapsilosis isolates are placed in one of the clusters, with a clustering rate of 53.8%. Among 16 C. glabrata isolates, 11 different genotypes were detected and divided into four clusters. Nine out of the 16 C. glabrata isolates are placed in one of the clusters, with a clustering rate of 56.2%. Conclusion: Our data indicated the possibility of nosocomial transmission of C. parapsilosis and C. glabrata among intensive care unit patients in our hospital. Infection control policies should be strictly applied in our hospital to prevent cross-transmission.https://www.hasekidergisi.com/articles/investigation-of-clonal-relationship-between-lessemgreatercandida-parapsilosislessemgreater-and-lessemgreatercandida-glabratalessemgreater-strains-isolated-from-blood-culture-by-pulse-field-gel-electrophoresis/doi/haseki.galenos.2025.36844candida parapsilosiscandida glabratafluconazole susceptibilitygenotype |
| spellingShingle | Ayse Alici Elif Seren Tanriverdi Gulgun Yenisehirli Baris Otlu Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis Haseki Tıp Bülteni candida parapsilosis candida glabrata fluconazole susceptibility genotype |
| title | Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis |
| title_full | Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis |
| title_fullStr | Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis |
| title_full_unstemmed | Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis |
| title_short | Investigation of Clonal Relationship Between Candida Parapsilosis and Candida Glabrata Strains Isolated from Blood Culture by Pulse Field Gel Electrophoresis |
| title_sort | investigation of clonal relationship between candida parapsilosis and candida glabrata strains isolated from blood culture by pulse field gel electrophoresis |
| topic | candida parapsilosis candida glabrata fluconazole susceptibility genotype |
| url | https://www.hasekidergisi.com/articles/investigation-of-clonal-relationship-between-lessemgreatercandida-parapsilosislessemgreater-and-lessemgreatercandida-glabratalessemgreater-strains-isolated-from-blood-culture-by-pulse-field-gel-electrophoresis/doi/haseki.galenos.2025.36844 |
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