EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae

The in vitro probiotic capacity of a native strain of Saccharomyces cerevisiae (A) was evaluated and comparedwith a commercial strain (B) used as a probiotic. The effect of the concentration of sugarcane molasses (10, 20and 30% (w/v)) on the biomass production was investigated and kinetic parameters...

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Main Authors: Ángela Ortiz, Joanna Reuto, Erika Fajardo, Sandra Sarmiento, Andrea Aguirre, Gustavo Arbeláez, David Gómez, Balkys Quevedo-Hidalgo
Format: Article
Language:English
Published: Pontificia Universidad Javeriana 2008-09-01
Series:Universitas Scientiarum
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Online Access:http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1418/880
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author Ángela Ortiz
Joanna Reuto
Erika Fajardo
Sandra Sarmiento
Andrea Aguirre
Gustavo Arbeláez
David Gómez
Balkys Quevedo-Hidalgo
author_facet Ángela Ortiz
Joanna Reuto
Erika Fajardo
Sandra Sarmiento
Andrea Aguirre
Gustavo Arbeláez
David Gómez
Balkys Quevedo-Hidalgo
author_sort Ángela Ortiz
collection DOAJ
description The in vitro probiotic capacity of a native strain of Saccharomyces cerevisiae (A) was evaluated and comparedwith a commercial strain (B) used as a probiotic. The effect of the concentration of sugarcane molasses (10, 20and 30% (w/v)) on the biomass production was investigated and kinetic parameters were determined. The bestmolasses concentration was 20% (w/v) and differences in biomass production on molasses medium betweenstrain A (28 g/L) and control strain B (3 g/L) were observed. In vitro tests such as tolerance to bile salts, pH andgastric juices were carried out, and no differences in growth between strain A and B were found. Cholesterolreduction on presence of bile salts after 12 hours of incubation was of 54% for strain A and 58% for strain B.Both strains showed adherence to Caco-2 cells. Results reveal that strain A possesses in vitro probiotic propertiesthat can be verified with further in vivo studies to confirm its suitability as probiotic in animal nutrition.
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publishDate 2008-09-01
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spelling doaj-art-dc636f14bc84487582cfe4656ebff6c82025-08-20T03:52:25ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522008-09-01132138148EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiaeÁngela OrtizJoanna ReutoErika FajardoSandra SarmientoAndrea AguirreGustavo ArbeláezDavid GómezBalkys Quevedo-HidalgoThe in vitro probiotic capacity of a native strain of Saccharomyces cerevisiae (A) was evaluated and comparedwith a commercial strain (B) used as a probiotic. The effect of the concentration of sugarcane molasses (10, 20and 30% (w/v)) on the biomass production was investigated and kinetic parameters were determined. The bestmolasses concentration was 20% (w/v) and differences in biomass production on molasses medium betweenstrain A (28 g/L) and control strain B (3 g/L) were observed. In vitro tests such as tolerance to bile salts, pH andgastric juices were carried out, and no differences in growth between strain A and B were found. Cholesterolreduction on presence of bile salts after 12 hours of incubation was of 54% for strain A and 58% for strain B.Both strains showed adherence to Caco-2 cells. Results reveal that strain A possesses in vitro probiotic propertiesthat can be verified with further in vivo studies to confirm its suitability as probiotic in animal nutrition.http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1418/880AdherenceBiomassSugarcane molassesProbioticSaccharomycesTolerance
spellingShingle Ángela Ortiz
Joanna Reuto
Erika Fajardo
Sandra Sarmiento
Andrea Aguirre
Gustavo Arbeláez
David Gómez
Balkys Quevedo-Hidalgo
EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae
Universitas Scientiarum
Adherence
Biomass
Sugarcane molasses
Probiotic
Saccharomyces
Tolerance
title EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae
title_full EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae
title_fullStr EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae
title_full_unstemmed EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae
title_short EVALUACIÓN DE LA CAPACIDAD PROBIÓTICA “IN VITRO” DE UNA CEPA NATIVA DE Saccharomyces cerevisiae
title_sort evaluacion de la capacidad probiotica in vitro de una cepa nativa de saccharomyces cerevisiae
topic Adherence
Biomass
Sugarcane molasses
Probiotic
Saccharomyces
Tolerance
url http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1418/880
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